Validation of SNPs Identified by Amplicon Sequencing and their Association with Egg Production and Egg Weight in Anand Synthetic White Leghorn and Anand Bantamised White Leghorn Chicken

Authors

  • Ayushi R. Patel Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India
  • Mayank R. Patel Department of Animal Genetics &Animal Breeding, College of Veterinary Science &Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India
  • Akash G Golaviya Department of Veterinary Microbiology, College of Veterinary Science &Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India
  • Subhash J Jhakesara Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India
  • Ashish C Patel Department of Animal Genetics &Animal Breeding, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India
  • Prakash G Koringa Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India

DOI:

https://doi.org/10.48165/ijvsbt.18.5.21

Keywords:

Chicken, Egg Production, Egg Weight, PCR-RFLP, SNP validation, Real-Time PCR

Abstract

The present study was aimed to validate the previously found SNPs using NGS-based amplicon sequencing and investigate of their association with egg production and egg weight by PCR-RFLP in Anand Synthetic White Leghorn (ASWLH) and Anand Bantamised White Leghorn (ABWLH) chicken. A total of 500 blood samples were collected including, 227 samples of ASWLH and 273 of ABWLH maintained at the Poultry Research Station, Anand Agricultural University, Anand, Gujarat. The study was conducted for a total of eight SNP markers located across the four genes involved in physiological pathways related to egg production and egg weight, viz., GnRHII (T88170424C), GnRHII (G88170427A), VIPR1 (C1826542A), VIPR1 (C1826555T), OCX32 (C21912308T), OCX32 (T21912084G), OCX32 (C21912423A) and GHR (C13415980T). Out of eight SNPs, C21912423A of the OCX32 gene was significantly associated with egg weight at the 40 weeks of age (EW40) in the whole population. The G88170427A of the GnRHII gene was significantly associated with egg weight at the 28 weeks of age (EW28) and C21912308T of the OCX-32 gene was significantly associated with total egg number at the age of 64 weeks (TWN64) in the whole population.

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References

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Published

2022-11-07

Issue

Vol. 18 No. 5 (2022): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Nov- Dec) 2022

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Patel, A.R., Patel, M.R., Golaviya, A.G., Jhakesara, S.J., Patel, A.C., & Koringa, P.G. (2022). Validation of SNPs Identified by Amplicon Sequencing and their Association with Egg Production and Egg Weight in Anand Synthetic White Leghorn and Anand Bantamised White Leghorn Chicken. Indian Journal of Veterinary Sciences and Biotechnology, 18(5), 104–110. https://doi.org/10.48165/ijvsbt.18.5.21
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