Molecular Detection and Phylogenetic Analysis of Mycoplasma conjunctivae from Ocular Infections of Goats in and around Tirupati Region

Authors

  • Sreelekha Madiri M.V.Sc Scholar, Department of Veterinary Microbiology, College of Veterinary Science, SVVU, Tirupati-517502, Andhra Pradesh, India.
  • Vijayalakshmi Siddavatam Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Proddatur-516361 Andhra Pradesh, India
  • Nagendra Reddy Topireddy Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517502, Andhra Pradesh, India
  • Sujatha Karmala Department of Veterinary Pathology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517502, Andhra Pradesh, India
  • Suresh Yasarla Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517502, Andhra Pradesh, India

DOI:

https://doi.org/10.48165/ijvsbt.22.3.34

Keywords:

Goats, Infectious keratoconjunctivitis, Molecular Detection, Mycoplasma conjunctivae, Phylogenetic analysis.

Abstract

Mycoplasma-associated infectious keratoconjunctivitis (IKC) represents one of the common ocular infections among ruminants.  Mycoplasma conjunctivae mainly causes infectious kerato-conjunctivitis (IKC) in goats, representing a highly transmissible condition  marked by differing levels of visual impairment in affected individuals. In the current study, a total of 39 conjunctival swabs were  collected from affected goats in and around the Tirupati region. Molecular detection was done by genus-specific and species-specific  PCR targeting the 16S rRNA gene, yielding 270 bp and 748 bp products, respectively. Phylogenetic analysis of the partial 16S rRNA gene  sequence of one of the obtained Mycoplasma conjunctivae isolates from goats revealed the genetic relatedness to distinct clade formed  by sheep ocular swab isolate from Brazil (MK 656520.1) and clustered with a goat isolate from Kerala, India (MW425380.1).

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References

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Published

2026-04-22

Issue

Vol. 22 No. 3 (2026): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (May-Jun) 2026

Section

Short Communication

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Copyright (c) 2026 Indian Journal of Veterinary Sciences and Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Madiri, S., Siddavatam, V., Topireddy, N. R., Karmala, S., & Yasarla, S. (2026). Molecular Detection and Phylogenetic Analysis of Mycoplasma conjunctivae from Ocular Infections of Goats in and around Tirupati Region. Indian Journal of Veterinary Sciences and Biotechnology, 22(3), 171-174. https://doi.org/10.48165/ijvsbt.22.3.34