Molecular Detection of Mycoplasma ovipneumoniae from Respiratory and Non-Respiratory Infections of Goats in and around Tirupati Region
DOI:
https://doi.org/10.48165/ijvsbt.22.3.24Keywords:
Goats, Incidence, Molecular Detection, Mycoplasma ovipneumoniae, Phylogenetic analysis.Abstract
Mycoplasmosis is a significant contagious disease that adversely impacts the economy of small ruminant farming. Mycoplasma ovipneumoniae is one of the major etiological agents associated with atypical pleuropneumonia and keratoconjunctivitis in goats. In the current study, a total of 238 samples (128 nasal swabs, 39 conjunctival swabs, 15 mastitis milk, 5 arthritis fluids, 45 lung tissues and 6 pleural fluids) were collected from affected goats and slaughter houses in and around Tirupati region (India). Molecular detection was done by genus-specific and species-specific PCR targeting the 16S rRNA gene, yielding 270 bp and 361 bp products, respectively. The overall incidence of genus Mycoplasma and M. ovipneumoniae was 61.76% and 19.32%, respectively. Phylogenetic analysis of the present isolate TPTMOVI L1, clustered with clade formed by goat nasal swab isolates from Andhra Pradesh (MK 138543.1 and MK182761.1) and segregated into distinct cluster with sheep lung isolate from USA (PQ 631135.1). The isolate showed genetic relatedness with goat isolates from Andhra Pradesh and USA. Notably, the detection of M. ovipneumoniae from conjunctival swabs confirms its involvement in ocular infections, particularly keratoconjunctivitis, in addition to respiratory disease. Higher detection rates were observed in nasal swabs and lung tissue samples, indicating the predominance of respiratory involvement, while ocular positivity highlights the multifactorial clinical presentation of the infection.
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