Isolation and Histopathological Studies of Fowl Adenovirus from Inclusion Body Hepatitis Suspected Chicken
DOI:
https://doi.org/0.48165/ijvsbt.22.3.11Keywords:
Cytopathic effects (CPE), Fowl adenovirus (FAdV), Inclusion body hepatitis (IBH), Isolation, Serotype FAdV-11.Abstract
Inclusion body hepatitis (IBH) is one of the major economically significant emerging diseases in broilers caused by various serotypes of fowl adenovirus (FAdV). Routine outbreaks were recorded, despite regular vaccination in breeders and young chicks. The aim of the present study was isolation, molecular confirmation and histopathological studies of FAdV associated with IBH. A total of 34 pooled liver samples of IBH suspected broiler, broiler breeder, layer and desi chickens were collected from different districts of Andhra Pradesh. Liver samples were initially screened by PCR and histopathology of liver tissues. Among these, 19 samples were found to be positive for L1 region of partial hexon gene in PCR, of which 5 were sequenced. These samples showed more than 99% sequence homology with serotype FAdV-11 of species D. These FAdV positive and sequenced liver tissues were selected for isolation in SPF embryonated chicken eggs (ECE) and chicken embryo liver (CEL) primary cell cultures. Infected embryos displayed haemorrhagic, shrunken with enlarged, mottled livers with the presence of basophilic intra-nuclear inclusion bodies within hepatocytes, while CEL primary cell culture exhibited characteristic cytopathic effects, including rounding, aggregation, clumping, detachment and floating. The presence of the virus was further identified by PCR and histopathology.Downloads
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