Molecular Detection and Phylogenetic Analysis of Canine Bufavirus Associated with Canine Parvovirus in Nagpur
DOI:
https://doi.org/10.48165/ijvsbt.22.3.06Keywords:
Canine bufavirus, Canine parvovirus, PCR, Phylogenetic analysis.Abstract
Viral enteritis is a major cause of mortality in dogs under six months of age. This study investigated the molecular characterisation of Canine bufavirus (CBuV) associated with Canine parvovirus (CPV) in faecal samples from dogs showing gastrointestinal illness. A total of 100 samples were screened, of which 46 were positive for CPV-2, indicating a prevalence of 46%. These CPV-positive samples were further tested for CBuV, and 3 samples were positive, showing a co-infection rate of 6.52%. Six representative samples (3 CPV-2 and 3 CBuV) were sequenced for phylogenetic analysis. The analysis showed a clear separation of CPV and CBuV into two major clades. All CPV sequences clustered within the CPV-2c lineage and showed 98.44%-99.03% homology with the Chinese strain MN119600. The CBuV isolates showed close genetic relationships with strains from Iran (99.93%), Thailand (98.72%), and China (99.12%), indicating genetic diversity among circulating CBuV strains.Downloads
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