Antimicrobial Resistance and Detection of Extended Spectrum β-Lactamase Producing Genes in Klebsiella Isolated from Buffalo Milk and Farm Environment

Authors

  • Prashant Prajapati Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Mhow-453441, M.P., India
  • Daljeet Kour Chhabra Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Mhow-453441, M.P., India
  • Rakhi Gangil Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Mhow-453441, M.P., India
  • Ravi Sikrodia Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Mhow-453441, M.P., India
  • Joycee Jogi Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Mhow-453441, M.P., India
  • Rakesh Sharda Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Mhow-453441, M.P., India

DOI:

https://doi.org/10.48165/ijvsbt.22.2.23

Keywords:

Antibiotic, ARGs, ESBL, Klebsiella, MDR.

Abstract

Klebsiella is an emerging pathogen of one health importance. It is ubiquitously distributed in environment as well as associated with humans and animals infections. Three hundred samples were collected for bacteriological isolation and identification of Klebsiella from buffalo mastitic milk and farm environment. All Klebsiella isolates were tested for susceptibility to 15 antibiotics belonging to different classes by disc diffusion method. Extended spectrum beta lactam (ESBL) Klebsiella and ESBL genes were also detected phenotypically by combined disc diffusion test and PCR, respectively. Klebsiella spp. were isolated from 15 (5.00%) out of 300 samples. Prevalence of both Klebsiella spp. and K. pneumoniae in buffaloes and farm environment was 4.66 and 5.33 %, respectively. All the Klebsiella isolates were simultaneously resistant towards 2 to 8 antibiotics. Eight isolates were found to be multidrug resistant (MDR). Two ESBL genes, viz., bla TEM and bla SHV were detected among 15 Klebsiella isolates and the prevalence was found to be 26.66 and 53.33 %, respectively. None of the bacterial isolates were positive for bla DHAM and bla CTX-M gene. It was recorded that multiple drug resistance (MDR) strains of Klebsiella and K. pneumoniae were prevalent in buffaloes and farm environment.

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Published

2026-03-10

Issue

Vol. 22 No. 2 (2026): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Mar-Apr) 2026

Section

Research Article

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Copyright (c) 2026 Indian Journal of Veterinary Sciences and Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Prajapati, P., Chhabra, D. K., Gangil, R., Sikrodia, R., Jogi, J., & Sharda , R. (2026). Antimicrobial Resistance and Detection of Extended Spectrum β-Lactamase Producing Genes in Klebsiella Isolated from Buffalo Milk and Farm Environment . Indian Journal of Veterinary Sciences and Biotechnology, 22(2), 125-130. https://doi.org/10.48165/ijvsbt.22.2.23