Effect of Intrauterine Probiotic Therapy on Fertility, Biochemical and Immunological Parameters in Subclinical Endometritis Affected Repeat Breeding Gir Cows

Authors

  • Nirbhay R Nandaniya MVSc Scholar, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Suryakant S Parikh Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Ramesh J Padodara Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Karsan B Vala Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Vishal S Suthar Animal Biotechnology Division, Gujarat Biotechnology University, Gandhinagar-382010, Gujarat, India
  • Mulraj D Odedara Cattle Breeding Farm, Kamdhenu University, Junagadh-362001, Gujarat, India

DOI:

https://doi.org/10.48165/ijvsbt.22.2.21

Keywords:

Antibiotic, Biochemical profile, Fertility, Gir cows, Probiotic, Subclinical endometritis.

Abstract

The present study was undertaken to evaluate the effect of probiotic therapy on fertility performance and associated biochemical and immunological parameters in subclinical endometritis (SCE) affected repeat breeding Gir cows. SCE affected cows (n=10 each) were allotted into probiotic (Lactobacillus plantarum, 10¹¹ cfu/30 mL saline, I/U once), antibiotic (ciprofloxacin 30 mL, I/U, thrice at 24 h interval), positive control (normal saline, 30 mL I/U once), and healthy control (no intervention) groups. Fertility response was assessed through conception rate up to three inseminations, while biochemical and immunological changes were evaluated at estrus before treatment, on day 5, and at subsequent estrus. The cumulative conception rate over three inseminations was highest in the probiotic treated group (70.00%), followed by healthy control (60.00%) and ciprofloxacin treated group (50.00%), whereas the untreated SCE group recorded the lowest conception rate (20.00%). Probiotic treated cows showed significant improvement (p<0.05) in blood glucose, total protein, calcium, and progesterone concentrations, along with a significant reduction (p<0.05) in serum urea levels.Immunological evaluation revealed a significant decrease (p<0.05) in serum interleukin-10 and serum amyloid-A concentrations in probiotic and antibiotic treated groups, indicating reduced uterine inflammatory response. The probiotic group consistently exhibited better biochemical recovery and fertility performance compared to untreated SCE cows.The study concludes that intrauterine probiotic therapy using Lactobacillus plantarum significantly improves reproductive efficiency, metabolic status, and inflammatory profile in subclinical endometritis affected repeat breeding Gir cows.

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References

Ahmadi MR, Mogheiseh A, Nazifi S. Changes in biomarkers serum amyloid-A and haptoglobin following treatment of endometritis in dairy cows. Comp Clin Pathol. 2018;27(6):1659-1665.

Barlund CS, Carruthers TD, Waldner CL, Palmer CW. A comparison of diagnostic techniques for postpartum endometritis in dairy cattle. Theriogenology. 2008;69(6):714-723.

Barrio M, Vigo M, Quintela LA, Becerra JJ, Garcia-Herradon PJ, Bello DM, et al. Influence of subclinical endometritis on the reproductive performance of dairy cows. Span J Agric Res. 2015;13(4):1-6.

Bhat FA, Bhattacharya HK, Hussain A, Nadeem M, Wani AR. Microbial profile, antibiogram and conception rate following treatment in repeat breeder cows. Intas Polivet. 2013;14(1):42-48.

Biswal SS, Das S, Balasubramanian S, Mohanty DN, Sethy K, Dasgupta M. Serum amyloid-A and haptoglobin levels in crossbred cows with endometritis following different therapy. Vet World. 2014;7(12):1066-1070.

Brodzki P, Kostro K, Krakowski L, Marczuk J. Inflammatory cytokine and acute phase protein concentrations in the peripheral blood and uterine washings of cows with subclinical endometritis in the late postpartum period. Vet Res Commun. 2015;39:143-149.

Chaudhari RJ, Gulavane SU, Rangnekar MN, Shelar RR, Gaikwad SM, Jawale RS. Subclinical endometritis in crossbred cows with special reference to blood biochemistry and reproductive parameters. Haryana Vet. 2020;59(SI):41-44.

Das S, Mishra SK, Swain RK, Mohanty DN, Mishra SR. Comparative study of certain serum biochemical parameters in anoestrus and repeat breeding cows of Bhadrak district of Orissa. Indian J Field Vet. 2012;7(4):62-65.

Dobos A, Fodor I, Kreizinger Z, Makrai L, Denes B, Kiss I, et al. Infertility in dairy cows: possible bacterial and viral causes. Vet Stanica. 2022;53(1):35-43.

Genis S, Sanchez-Chardi A, Bach A, Fabregas F, Aris A. A combination of lactic acid bacteria regulates Escherichia coli infection and inflammation of the bovine endometrium. J Dairy Sci. 2017;100(1):479-492.

Gohil P, Nanavati B, Patel K, Suthar V, Joshi M, Patil DB, et al. Assessing the efficacy of probiotics in augmenting bovine reproductive health: an integrated in vitro, in silico and in vivo study. Front Microbiol. 2023;14:1-21.

Iancu I, Popa SA, Degi J, Gligor A, Popa I, Iorgoni V, et al. Aerobic uterine pathogens in dairy cattle: surveillance and antimicrobial resistance profiles in postpartum endometritis. Antibiotics (Basel). 2025;14(7):650-663.

Kaya S, Merhan O, Kacar C, Colak A, Bozukluhan K. Determination of ceruloplasmin, some other acute phase proteins and biochemical parameters in cows with endometritis. Vet World. 2016;9(10):1056.

Khalifa E, Zaki SA, Ghallab RS. Molecular identification and sequencing of uterine bacterial infection associated with subfertility problems and suggested treatment protocols in Arabian mares. Assiut Vet Med J. 2025;71(185):240-258.

Kumar M, Pant SS, Ramsharan R, Salendra K, Gupta PK. Therapeutic efficacy of levofloxacin along with vitamin-E for the management of repeat breeding syndrome in cows under field condition. Int J Vet Sci. 2014;3(3):155-157.

Mohames SH, Kumar SR, Sathiamoorthy T, Porteen K, Ravikumar R, Umamageshwary J, et al. Effect of intra-uterine administration of Lactobacillus bacteria on the steroid hormone profile in subclinical endometritis affected cows. Indian J Anim Sci. 2024;94(5):411-414.

Nanas I, Giannoulis T, Dovolou E, Giannenas I, Amiridis GS. Impact of low inclusion rate of olive cake in dairy cow rations on uterine health and fertility indices during early lactation. Animals (Basel). 2025;15(2):1-20.

Nischala S, Sireesha G. Serum proteins in endometritis affected buffaloes. J Livest Sci. 2018;9:81-83.

Parikh SS. Comparative studies on different diagnostic methods and therapeutic approaches for postpartum subclinical endometritis in Gir cows. PhD Thesis. Gujarat: Kamdhenu University; 2021.

Parkinson TJ. Infertility in the cow due to functional and management deficiencies. In: Noakes DE, Parkinson TJ, England GCW, editors. Veterinary reproduction and obstetrics. 10th ed. London: WB Saunders; 2019. p.361-407.

Perumal P, Chaurasia D, De AK, Charya D, Sunder J, Bhowmick S, et al. Effect of clinical endometritis on physiological, haematological, biochemical and endocrinological profiles in crossbred cows under tropical island ecosystem. Indian J Anim Sci. 2020;90(9):1296-1299.

Pothmann H, Prunner I, Wagener K, Jaureguiberry M, de la Sota RL, Erber R, et al. The prevalence of subclinical endometritis and intrauterine infections in repeat breeder cows. Theriogenology. 2015;83(8):1249-1253.

Prakash MS, Pathan MM, Arya JS, Lunagariya PM. Assessment of glucose, total protein, albumin and cholesterol level and its correlation with milk production during different stages of lactation in indigenous and crossbred cows. Int J Curr Microbiol Appl Sci. 2018;7(4):1248-1256.

Sarkar P, Kumar H, Kumar PM. Leucocytes enriched autologous plasma for the management of bovine endometritis. Intas Polivet. 2015;16(2):214-217.

Shivhare M, Singh S, Tayeng K, Chakravarti S. Endometrial cytology to diagnose subclinical endometritis in cows. Int J Agric Environ Biotechnol. 2018;11(4):623-631.

Suthar V, Dhami AJ, Gohil P, Joshi M, Patil DB, Joshi CG. Probiotics intervention for mitigation of uterine infection in dairy animals—an update. Anim Reprod Update. 2022;1(2):49-58.

Vadher DV, Parikh SS, Sharma AK, Vijyeta HP, Thummar MN, Ram DR. Effect of ashwagandha, turmeric and antibiotic on blood biochemical profile of repeat breeding Gir cows with subclinical endometritis. Indian J Vet Sci Biotechnol. 2025;21(2):7-12.

Vallejo-Timaran D, Montoya-Zuluaga J, Castillo-Vanegas V, Maldonado-Estrada J. Parity and season affect haematological, biochemical and milk parameters during the early postpartum period in grazing dairy cows from high tropics herds. Heliyon. 2020;6(5):1-8.

Yang L, Huang W, Yang C, Ma T, Hou Q, Sun Z, et al. Using PacBio sequencing to investigate the effects of treatment with lactic acid bacteria or antibiotics on cow endometritis. Electron J Biotechnol. 2021;51:67-78.

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Published

2026-03-10

Issue

Vol. 22 No. 2 (2026): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Mar-Apr) 2026

Section

Research Article

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Copyright (c) 2026 Indian Journal of Veterinary Sciences and Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Nandaniya, N. R., Parikh, S. S., Padodara, R. J., Vala, K. B., Suthar, V. S., & Odedara, M. D. (2026). Effect of Intrauterine Probiotic Therapy on Fertility, Biochemical and Immunological Parameters in Subclinical Endometritis Affected Repeat Breeding Gir Cows. Indian Journal of Veterinary Sciences and Biotechnology, 22(2), 115-120. https://doi.org/10.48165/ijvsbt.22.2.21