Evaluation of Biofilm formation capacity of Pasteurella multocida and its relationship with antibiotic resistance

Authors

  • Kavitha Kandimalla Department of Veterinary Microbiology and Biotechnology, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana
  • Basavaraj Awati Department of Veterinary Microbiology, Veterinary College, Bidar, KVAFSU, Bidar, Karnataka.
  • Kalyani Putty Department of Veterinary Microbiology and Biotechnology, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana
  • Vamshi Krishna Sai Ram Department of Veterinary Microbiology and Biotechnology, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana
  • Narsimha Reddy Yella Department of Veterinary Microbiology and Biotechnology, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana
  • Nanagouda A Patil Department of Veterinary Medicine, Veterinary College, Bidar, Karnataka.
  • Ravindra Bhoyar Department of Veterinary Medicine, Veterinary College, Shivamogga, Karnataka.
  • Mallinath Karabasappa Choudapur Department of Veterinary Microbiology, Veterinary College, Bidar, KVAFSU, Bidar, Karnataka.
  • Arun Karate Department of Veterinary Microbiology, Veterinary College, Bidar, KVAFSU, Bidar, Karnataka.
  • Gopala Lunavat Department of Veterinary Microbiology and Biotechnology, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana
  • Jayashri Akkaldevi Department of Veterinary Biochemistry, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana
  • Vishweshwar Ganji Department of Veterinary Microbiology and Biotechnology, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana

DOI:

https://doi.org/10.48165/ijvsbt.18.5.14

Keywords:

Biofilm, Haemorrhagic Septicaemia, Pasteurella multocida, Real-time PCR, tad locus genes

Abstract

Pasteurella multocida is a Gram-negative bacterium that causes haemorrhagic septicaemia in cattle and buffaloes. These organisms are involved in the formation of biofilm and can evade treatment. There is no definitive study to screen the genes associated with biofilm production associated with Pasteurella multocida. The present study describes the real-time PCR based approach for screening of genes associated with biofilm formation. Out of 10 isolates screened for biofilm formation, five of them produced biofilm on Congo red agar of which all are resistant to major antibiotics especially cotrimoxazole, nalidixic acid, enrofloxacin and tetracyclines. All the isolates show presence of genes associated with biofilm formation indicating that other factors influencing the biofilm production. We suggest that the future studies may be targeted to unravel the other factors that influence the biofilm production.

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References

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Published

2022-11-07

Issue

Vol. 18 No. 5 (2022): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Nov- Dec) 2022

Section

Articles

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Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Kandimalla, K., Awati, B., Putty, .K., Ram, V.K.S., Yella, N.R., Patil, N.A., … Ganji, V. (2022). Evaluation of Biofilm formation capacity of Pasteurella multocida and its relationship with antibiotic resistance. Indian Journal of Veterinary Sciences and Biotechnology, 18(5), 68–74. https://doi.org/10.48165/ijvsbt.18.5.14
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