Effect of Sperm Preparation Methods on In-vitro Fertilization of Goat Oocytes

Authors

  • Hawanagoudar Vinutha M.V.Sc. Scholar, Department of Animal Reproduction Gynaecology & Obstetrics, Veterinary College, Bidar-585401, KVAFSU, Karnataka, India.
  • Rajeshwar Gangaram Bijurkar Department of Veterinary Clinical Complex, Veterinary College, Bidar-585401, KVAFSU, Karnataka, India
  • Manik Kishanrao Tandle Department of Animal Reproduction Gynaecology & Obstetrics, Veterinary College, Bidar-585401, KVAFSU, Karnataka, India
  • Shrikant Kulkarni Department of Veterinary Physiology and Biochemistry, Veterinary College, Bidar-585401, KVAFSU, Karnataka, India
  • Prashantkumar Waghe Department of Veterinary Pharmacology and Toxicology, Veterinary College, Bidar-585401, KVAFSU, Karnataka, India
  • Doddagoudar Venkanagouda Department of Animal Reproduction Gynaecology & Obstetrics, Veterinary College, Bidar-585401, KVAFSU, Karnataka, India
  • Malasri Gond Department of Animal Reproduction Gynaecology & Obstetrics, Veterinary College, Bidar-585401, KVAFSU, Karnataka, India

DOI:

https://doi.org/10.48165/ijvsbt.22.2.14

Keywords:

Goat oocytes, In-vitro Fertilization, Percoll, Swim up, Sperm preparation.

Abstract

The present study was undertaken to evaluate the effect of different sperm preparation methods, viz., Percoll density gradient, Swim up and Simple Washing by centrifugation, on in-vitro fertilization (IVF) of goat oocytes. Ovaries obtained from slaughtered goats were used to recover cumulus–oocyte complexes (COCs) by the slicing method, and oocytes were graded based on cumulus cell layers and cytoplasmic characteristics. Grade A and B oocytes were selected for in-vitro maturation (IVM) and then subjected to IVF using spermatozoa processed through the three preparation methods. Fertilization efficiency was assessed after in-vitro culture. Among the treatments, percoll density gradient yielded the highest cleavage rate (51.11 ± 3.15%), followed by swim up (45.47 ± 4.27%) and simple washing (38.56 ± 3.98%) (p<0.05). These findings indicate that sperm preparation technique significantly influences IVF outcome in goats, emphasizing the importance of sperm functional quality over numerical sperm recovery.

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Published

2026-03-10

Issue

Vol. 22 No. 2 (2026): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Mar-Apr) 2026

Section

Research Article

License

Copyright (c) 2026 Indian Journal of Veterinary Sciences and Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Vinutha, H., Bijurkar, R. G., Tandle, M. K., Kulkarni, S., Waghe, P., Venkanagouda, D., & Gond, M. (2026). Effect of Sperm Preparation Methods on In-vitro Fertilization of Goat Oocytes . Indian Journal of Veterinary Sciences and Biotechnology, 22(2), 77-80. https://doi.org/10.48165/ijvsbt.22.2.14