Effect of Epidermal Growth Factor Supplementation and Different Fertilization Media on In Vitro Maturation and Fertilization of Goat Oocytes
DOI:
https://doi.org/10.48165/ijvsbt.22.2.02Keywords:
BO-IVF, EGF, Fert-TALP, Goat oocytes, In vitro fertilisation In vitro maturation, SOFAbstract
The present study evaluated the effect of epidermal growth factor (EGF) supplementation during in vitro maturation (IVM) and different fertilization media on nuclear maturation and fertilization rates of goat oocytes. Ovaries were collected from a local abattoir and oocytes were recovered by the slicing method. A total of 386 grade A and B oocytes were selected and matured in two media: medium I (TCM‑199 + FSH 1 µg/mL + hCG 20 IU/mL + Estradiol 1 µg/mL + Gentamicin 50 µg/mL + 10% EGS -Estrus Goat Serum) and medium II (medium I + EGF -Epidermal Growth Factor, 20 ng/mL). Oocytes were incubated for 27 h at 38.5°C with 5% CO2 in incubator. A proportion of the oocytes was fixed and stained with 1% aceto‑orcein to assess nuclear maturation to metaphase II (M II). The remaining oocytes were fertilized in three different media (Fert‑TALP, BO‑IVF and SOF) using fresh buck semen for 18 h, and total penetration (2 pronuclei plus polyspermy) was evaluated. The proportion of oocytes reaching M II was significantly higher (p < 0.05) in the EGF‑supplemented group than in the control (52.13 ± 3.19% vs. 16.16 ± 0.50%, respectively). Among EGF‑matured oocytes, total penetration rate was significantly higher (p < 0.05) in BO‑IVF medium (73.75 ± 5.25%) than in Fert‑TALP (47.72 ± 7.14%) and SOF (20.98 ± 7.79%). In conclusion, supplementation of IVM medium with EGF combined with fertilization in BO‑IVF medium improves nuclear maturation and fertilization rates of goat oocytes and may be beneficial for in vitro embryo production.Downloads
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