Comparative Efficacy of Kisspeptin and GnRH for Ovulation Synchronization in Cattle
DOI:
https://doi.org/10.48165/ijvsbt.22.3.18Keywords:
Conception rate, Gonadotropins, Kisspeptin-10, Ovsynch protocol, Ovulation synchronization, Postpartum dairy cows.Abstract
The aim of the study was to investigate the potential of Kisspeptin-10 as an alternative to GnRH in the Ovsynch protocol for improving reproductive performance in postpartum dairy cows. A total of 20 postpartum dairy cows were selected and the animals were equally divided into two groups. In Group T1, cows were treated with Kisspeptin-10 on Day 0, PGF2α on Day 7 and Kisspeptin-10 on Day 9. In Group T2, cows were treated with the conventional Ovsynch protocol consisting of GnRH on Day 0, PGF2α on Day 7 and GnRH on Day 9. Timed artificial insemination was performed 18-24 h after the final hormonal injection. Estrus attributes, pregnancy rate, and serum FSH and LH concentrations were assessed. Although the percentage of induced estrus response and the onset time for estrus after PGF2α injection were comparable between groups, kisspeptin-treated cows exhibited a significantly longer duration of estrus. The pregnancy rate was higher in the Kisspeptin-10 treated group than in the GnRH treated group (50.0 vs. 30.0 %). Endocrine evaluation revealed significantly higher FSH levels in GnRH-treated cows, whereas kisspeptin-treated cows showed a significantly higher LH response at 30 min post-injection on Day 9. Overall, incorporation of Kisspeptin-10 into the Ovsynch protocol may serve as an effective alternative to GnRH for ovulation synchronization in postpartum dairy cows.
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