Molecular Characterization and Antibiogram of Pseudomonas aeruginosa Isolated from Various Meat Samples in and around Tirupati, Andhra Pradesh
DOI:
https://doi.org/10.48165/ijvsbt.22.3.03Keywords:
Antimicrobial resistance, ESKAPE, PCR, Pseudomonas aeruginosa, rpoB, 16S rRNA.Abstract
Pseudomonas aeruginosa is an emerging opportunistic pathogen and is one of the pathogens in the acronym ESKAPE highlighting a global threat to human health due to antimicrobial resistance. This study aimed to molecularly characterize P. aeruginosa, and to assess its antimicrobial resistance in different meat samples, including chicken, beef, pork, frozen chicken, frozen beef and frozen pork, collected from various meat markets in Tirupati, Andhra Pradesh, India. A total of 18 (16.07%) P. aeruginosa isolates were confirmed from 112 meat samples, viz., chicken (18.75 %), beef (16.6%), pork (20 %), and frozen pork (20%) by PCR targeting the 16S rRNA and rpoB genes, respectively. None of the isolates were identified in frozen chicken and frozen beef samples. Further, all the 18 P. aeruginosa isolateswere phenotypically characterized for antimicrobial resistance patterns using the disc diffusion method and found 100% resistance to erythromycin, 17 isolates were resistance against amoxycillin (94%), 15 isolates to tertracycline and vancomycin (83%), 14 isolates to cotrimoxazole (77.7%), 8 isolates against ciprofloxacin (44%) and 4 isolates were resistance against gentamicin (22 %). The presence of multiple antibiotic-resistant P. aeruginosa, which carry virulence genes, in various meat samples, poses a significant public health risk from consuming raw or undercooked meat. Consequently, the detection of these resistant strains in meat highlights the urgent need for improved food safety measures and monitoring to protect public health.
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