Antimicrobial and Antioxidant Activity of Spices (Garlic,  Cinnamon, Clove) in Minced Chicken at Refrigeration Storage

Authors

  • Bhoomi A Solanki Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada-385506, Kamdhenu University, Gujarat, India
  • Bhavesh I Prajapati Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, Anand-388001, Kamdhenu University, Gujarat, India
  • Mayank M Goswami Department of Livestock Products Technology, College of Veterinary Science and Animal Husbandry, Rajpur (Nava), Himmatnagar-383010, Kamdhenu University, Gujarat, India

DOI:

https://doi.org/10.48165/ijvsbt.22.2.10

Keywords:

Chicken, Clostridium perfringens, Escherichia coli, Natural preservatives, Nitrite, Staphylococcus aureus.

Abstract

The present study was conducted to evaluate antioxidant and antimicrobial effect of garlic, cinnamon and clove as nitrite replacer in  chicken mince meat stored at refrigeration temperature (4±1°C). Chemical characteristics (pH, TBARS) were assessed on 0, 3, 6 and 9th day of storage, while antimicrobial studies (Escherichia coli, Staphylococcus aureus and Clostridium perfringens) were carried out on 1, 4, 7  and 10th day. The pH values increased gradually and significantly (p<0.05) over each storage interval in all treatments and control; it was  highest in T3 (0.2% clove powder) and lowest in T2 (2% w/w cinnamon powder) or T1 (3% garlic paste), while in control and N treated  samples it was intermediate. TBA values were significantly (p<0.05) lower in T1 followed by N, T2 and T3, and highest in control samples  at all storage intervals, and increased significantly with each storage interval from day 0 to 9. Inclusion of Garlic paste (3 %), Cinnamon  powder (2 % w/w) and Clove powder (0.2 % w/w) in chicken mince meat was significantly effective (p≤0.05) against Escherichia coli and Staphylococcus aureus, but had slightly lower effect against Clostridium perfringens. In conclusion, clove having good antioxidant  activity in complex food system like chicken mince where nitrite can be replaced with clove. Antimicrobial activity of clove powder was  higher against S. aureus, E.coli and C. perfringenes count as compared to control. 

 

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References

Amin M, Oliviera JV. The effect of the use of nitrite and nitrate on the inhibition of Clostridium perfringens type A on bovine cured sausages. Bol Cent Pesqui Process Aliment. 2006;24(1):13-24.

Ankri S, Mirelman D. Antimicrobial properties of allicin from garlic. Microbes Infect. 1999;2:125-129.

Cheng SS, Liu JY, Tsai KH, Chen WJ, Chang ST. Chemical composition and mosquito larvicidal activity of essential oils from leaves of different Cinnamomum osmophloeum provenances. J Agric Food Chem. 2004;52:4395-4400.

Douglas BM, Donald SM, Douglas NR. Contribution of nitrite and nitrate to the colour and flavour of cured meats. J Sci Food Agric. 1975;26:1743-1754.

Dwivedi S, Vasavada MN, Cornforth D. Evaluation of antioxidant effects and sensory attributes of Chinese five-spice ingredients in cooked ground beef. J Food Sci. 2006;71:12-17.

Erich S. Composition of antioxidant activity of essential oil of cinnamon. J Essent Oil Bear Plants. 2006;9(2):170-182.

Friedman M, Henika PR, Mandrell RE. Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes and Salmonella enterica. J Food Prot. 2002;65:1545-1560.

Food Safety and Standards Authority of India. Food products standards and food additives regulations. New Delhi: FSSAI; 2011. p.287-447.

Gaysinsky S, Weiss J. Aromatic and spice plants: uses in food safety. Stewart Postharvest Rev. 2007;4:9-16.

Gordon MH. The mechanism of antioxidant action in vitro. In: Hudson BJF, editor. Food antioxidants. New York: Elsevier Applied Science; 1990. p.1-18.

Goswami M, Prbhakaran P, Tanvar VK. Antioxidant and antimicrobial effects of condiments paste used as nitrite replacer in chicken mince. Vet World. 2014;7(6):432-438.

Govaris N, Solomakos A, Pexara A, Chatzopoulou PS. The antimicrobial effect of oregano essential oil, nisin and their combination against Salmonella enteritidis in minced sheep meat during refrigerated storage. Int J Food Microbiol. 2010;137:175-180.

Gupta C, Garg AP, Uniyal RC, Kumari A. Comparative analysis of the antimicrobial activity of cinnamon oil and cinnamon extract on some food borne microbes. Afr J Microbiol Res. 2008;2(9):247-251.

Han SK, Yamauchi K, Park HK. Effect of nitrite on lipid component and lipid oxidation in stored cured meat under refrigeration. J Anim Sci Technol. 2000;42(3):339-348.

Hernández-Ochoa L, Aguirre-Prieto YBG, Nevárez-Moorillón V, Gutierrez-Mendez N, Salas-Muñoz E. Use of essential oils and extracts from spices in meat protection. J Food Sci Technol. 2011;51(5):957-963.

Hossein N, Zahra Z, Abolfazl M, Mahdi S, Ali K. Effect of Cinnamomum zeylanicum essence and distillate on the clotting time. J Med Plants Res. 2013;7:1339-1343.

Jakszyn P, Gonzalez CA. Nitrosamine and related food intake and gastric and oesophageal cancer risk: a systematic review of the epidemiological evidence. World J Gastroenterol. 2006;12(27):4296-4303.

Karabagias I, Badeka A, Kontominas MG. Shelf life extension of lamb meat using thyme or oregano essential oils and modified atmosphere packaging. Meat Sci. 2011;88:109-116.

Kim J, Marshall MR, Wei C. Antibacterial activity of some essential oil components against five foodborne pathogens. J Agric Food Chem. 1995;43:2839-2845.

Masahiro O, Midori H, Shiro U, Toyoshige E. Antioxidant activity of eugenol and related monomeric and dimeric compounds. Chem Pharm Bull. 2000;48(10):1467-1469.

Morita H, Hiroshi Y. Antimicrobial action against verotoxigenic Escherichia coli O157:H7 of nitric oxide derived from sodium nitrite. Biosci Biotechnol Biochem. 2004;68(5):1027-1034.

Naveena BM, Muthukumar M, Sen AR, Babji Y, Murthy TRK. Improvement of shelf-life of buffalo meat using lactic acid, clove oil and vitamin C during retail display. Meat Sci. 2006;74:409-415.

Rees LP, Minney SF, Plummer NT, Slater JH, Skyrme DA. A quantitative assessment of the antimicrobial activity of garlic (Allium sativum). World J Microbiol Biotechnol. 1993;9:303-307.

Sebranek JG, Bacus JN. Cured meat products without direct addition of nitrate and nitrite: what are the issues? Meat Sci. 2007;77:136-147.

Seema A, Dostbil N, Alemdar S. Antimicrobial effect of some spices used in meat industry. Bull Vet Inst Pulawy. 2007;51:53-57.

Sharma J, Prabhakaram PP, Tamwar VK. Antioxidant and antimicrobial effect of turmeric and nitrite on minced chicken. J Anim Res. 2012;2(3):299-306.

Singh P, Sahoo J, Chatli MK, Biswas AK. Shelf-life evaluation of raw chicken meat emulsion incorporated with clove powder, ginger and garlic paste as natural preservatives at refrigerated storage (4±1°C). Int Food Res J. 2014;21(4):1363-1373.

Singh IA, Yadav AS, Pandey NK, Singh RP. Antimicrobial effect of spice extracts against Salmonella typhimurium in chicken patties during frozen storage and its detection by PCR method. In: International Poultry Science Association Conference; 2005 Nov; Indian Poultry Association. p.115-120.

Snedecor GW, Cochran WG. Statistical methods. 8th ed. Ames: Iowa State University Press; 1994. p.124-130.

Taormina PJ, Bartholomew GW, Dorsa WJ. Incidence of Clostridium perfringens in commercially produced cured raw meat product mixtures and behaviour in cooked products during chilling and refrigerated storage. J Food Prot. 2003;66(1):72-81.

Trout ES, Hunt MC, Johnson DE, Clans JR, Castner CL, Kropf DH. Characterization of low-fat ground beef containing texture modifying ingredients. J Food Sci. 1992;57(1):19-24.

Varel VH, Muller DL. Eugenol stimulates lactate accumulation yet inhibits volatile fatty acid production and eliminates coliform bacteria in cattle and swine waste. J Appl Microbiol. 2004;97(5):1001-1005.

Vipraja V, Keith U, Ingold D, Pratt A. Garlic: source of the ultimate antioxidant sulfenic acid. Angew Chem. 2008;121(1):161-163.

Witte VC, Krouze GF, Bailey ME. A new extraction method for determining 2-thiobarbituric acid values of pork and beef during storage. J Food Sci. 1970;35:582-585.

Yadav AS, Singh RP. Natural preservatives in poultry meat products. Nat Prod Radiance. 2004;3(4):300-303.

Yadav AS, Pandey NK, Singh RP. Studies on the use of natural preservatives for preservation of chicken meat. In: Proceedings of the XXIII Annual Conference of Indian Poultry Science Association and National Symposium; 2005. p.145-178.

Published

2026-03-10

How to Cite

Solanki, B. A., Prajapati, B. I., & Goswami, M. M. (2026). Antimicrobial and Antioxidant Activity of Spices (Garlic,  Cinnamon, Clove) in Minced Chicken at Refrigeration Storage. Indian Journal of Veterinary Sciences and Biotechnology, 22(2), 52-57. https://doi.org/10.48165/ijvsbt.22.2.10