Profiling the Bioactive Richness (Flavonoids, Phenolic Acids and Vitamins) of Acacia Leaves
DOI:
https://doi.org/10.48165/ijvsbt.22.1.38Keywords:
Acacia leaf extract, Flavonoids, LC-MS/MS profiling, Phenolic acids, PhytochemicalsAbstract
Phytochemicals derived from plants have gained considerable attention for their health-promoting properties, including antioxidant, anti inflammatory, and disease-preventing capabilities. This study focuses on the phytochemical profiling of Acacia leaf extracts, highlighting their potential as nutraceutical and animal health supplements. Using LC-MS/MS, we identified and quantified key flavonoids, phenolic acids, and vitamins present in Acacia leaves. Significant levels of bioactive flavonoids such as quercetin, rutin, hesperetin, naringenin, and luteolin were detected, with quercetin-3-rutinoside (rutin) showing the highest concentration. Phenolic profiling revealed abundant levels of gallic acid, p-coumaric acid, and dihydroxybenzoic acid, compounds known for their antioxidant and antimicrobial properties. In addition to phytochemicals, Acacia leaves were found to be rich in essential vitamins, particularly water-soluble B-complex vitamins (thiamine, niacin, pantothenic acid) and fat-soluble vitamins E and K1. These findings demonstrate the multifunctional value of Acacia leaf extracts in promoting health and productivity in both humans and animals. The results support further exploration of Acacia as a sustainable source of natural antioxidants and micronutrients for functional food, pharmaceutical, and animal feed applications.
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References
Bai, J., Zhang, Y., Tang, C., Hou, Y., Ai, X., Chen, X., … Meng, X. (2021). Gallic acid: Pharmacological activities and molecular mechanisms involved in inflammation-related diseases. Biomedicine & Pharmacotherapy, 133, 110985.
Bindawa, A. T., Lame, S. A., & Umar, S. M. (2022). Phytochemical screening and antioxidant vitamins of Acacia nilotica pod. Bima Journal of Science and Technology, 6(3), 121–128.
Chen, H., Zuo, Y., & Deng, Y. (2001). Separation and determination of flavonoids and other phenolic compounds in cranberry juice by high-performance liquid chromatography. Journal of Chromatography A, 913(1–2), 387–395.
Diwan, A. D., & Panche, A. N. C. S. R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5, 1–15.
Fang, F., Tang, K., & Huang, W. D. (2013). Changes of flavonol synthase and flavonol contents during grape berry development. European Food Research and Technology, 237(4), 529–540.
Jiang, B., Song, J., & Jin, Y. (2020). A flavonoid monomer tricin in gramineous plants: Metabolism, bio/chemosynthesis, biological properties, and toxicology. Food Chemistry, 320, 126617.
Joseph, N., Anjum, N., & Tripathi, Y. C. (2016). Phytochemical screening and evaluation of polyphenols, flavonoids and antioxidant activity of Prunus cerasoides D. Don leaves. Journal of Pharmacy Research, 10(7), 502–508.
Kumar, V., & Roy, B. K. (2018). Population authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis. Scientific Reports, 8(1), 10714.
Lin, D., Xiao, M., Zhao, J., Li, Z., Xing, B., Li, X., … Chen, S. (2016). An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules, 21(10), 1374.
Maji, S., & Modak, S. (2021). Neem: Treasure of natural phytochemicals. Chemical Science Review and Letters, 10(39), 396–401.
Martel, J., Ojcius, D. M., Ko, Y. F., Ke, P. Y., Wu, C. Y., Peng, H. H., & Young, J. D. (2019). Hormetic effects of phytochemicals on health and longevity. Trends in Endocrinology & Metabolism, 30(6), 335–346.
Mohamed, E. K., & Hafez, D. M. (2023). Gallic acid and metformin co-administration reduce oxidative stress, apoptosis and inflammation via Fas/caspase-3 and NF-κB signaling pathways in thioacetamide-induced acute hepatic encephalopathy in rats. BMC Complementary Medicine and Therapies, 23(1), 265.
Mulvihill, E. E., Burke, A. C., & Huff, M. W. (2016). Citrus flavonoids as regulators of lipoprotein metabolism and atherosclerosis. Annual Review of Nutrition, 36(1), 275–299.
Nollet, L. M. L., & Gutierrez-Uribe, J. A. (Eds.). (2018). Phenolic compounds in food: Characterization and analysis. CRC Press.
Rajanandh, M. G., & Kavitha, J. (2010). Quantitative estimation of β-sitosterol, total phenolic and flavonoid compounds in the leaves of Moringa oleifera. Pharmacognosy Journal, 2, 1409–1414.
Raju, S., Nagalakshmi, D., Nalini Kumari, N., Sravanthi, B., Ramyavasavi, M., & Divya, B. (2025). Acacia leaves: A promising alternative feed source in addressing feed shortages and enhancing livestock productivity. The Indian Journal of Veterinary Sciences & Biotechnology, 21(4), 99–103.
Rauf, A., Imran, M., Patel, S., Muzaffar, R., & Bawazeer, S. S. (2017). Rutin: Exploitation of the flavonol for health and homeostasis. Biomedicine & Pharmacotherapy, 96, 1559–1561.
Santos, J., Mendiola, J. A., Oliveira, M. B. P. P., Ibáñez, E., & Herrero, M. (2012). Sequential determination of fat- and water-soluble vitamins in green leafy vegetables during storage. Journal of Chromatography A, 1261, 179–188.
Sarkar, D., Christopher, A., & Shetty, K. (2022). Phenolic bioactives from plant-based foods for glycemic control. Frontiers in Endocrinology, 12, 727503.
Sharma, A., Shahzad, B., Rehman, A., Bhardwaj, R., Landi, M., & Zheng, B. (2019). Response of phenylpropanoid pathway and the role of polyphenols in plants under abiotic stress. Molecules, 24, 1–22.
Ul Haq, S., Khan, A., Ali, M., Khattak, A. M., Gai, W. X., Zhang, H. X., Wei, A. M., & Gong, Z. H. (2019). Heat shock proteins: Dynamic biomolecules to counter plant biotic and abiotic stresses. International Journal of Molecular Sciences, 20, 1–31.
Weidner, S., Amarowicz, R., Karamać, M., & Frączek, E. (2000). Changes in endogenous phenolic acids during development of Secale cereale caryopses and after dehydration treatment of unripe rye grains. Plant Physiology and Biochemistry, 38(7–8), 595–602.
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