Study on Prevalence and Antibiotic Profiling of Enterococcus Spp. from Chicken Meat
DOI:
https://doi.org/10.48165/ijvsbt.18.2.14%20Keywords:
Antibiotic resistance, Chicken meat, Enterococcus, PCRAbstract
The present study was undertaken to characterize Enterococcus species isolated from poultry meat by cultural isolation, PCR detection and antibiogram. Antibiotic profiling of detected Enterococcus spp. isolates were done by disc diffusion method. Total 150 chicken meat samples were examined for the presence of Enterococcus spp. The overall prevalence of Enterococcus spp. by cultural isolation was 56%. The prevalence of Enterococcus spp. by targeting tuf gene and ddlE.faecalis gene in PCR was 95.23 and 77.38%, respectively. In the antibiotic sensitivity test, Enterococcus isolates showed high resistance toward Erythromycin and Tetracycline. However, low resistance was seen towards Chloramphenicol, whereas all the isolates were susceptible to Gentamicin.This study found a high prevalence of multi drug resistance among enterococci isolated from poultry meat, suggesting that these resistant bacteria and genes may be transported from food to humans and pose a significant risk to human health in the coming years.
Downloads
References
Banik, A., Mohammad, N., Akter, T., Fatema, K., & Abony, M. (2018). Prevalence, identification and antibiotic susceptibility of Enterococcus Species isolated from chicken and pigeon meat in Gazipur area of Bangladesh. Open Journal of Medical Microbiology, 8(3), 74-83
Butaye P, Devriese LA,m & Haesebrouck F. (2003). Antimicrobial growth promoters used in animal feed: effects of less well known antibiotics on gram-positive bacteria. Clinical microbiology Reviews, 16(2): 175-188.
Baur, A.W., Kirby, W.M.M., Sherris, J.C.T., & Turch, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45(4), 493-496.
Cattoir, V. (2022). The multifaceted lifestyle of enterococci: genetic diversity, ecology and risks for public health. Current Opinion in Microbiology, 65, 73-80.
Clinical and Laboratory Standards Institute (2017). Performance Standards for Antimicrobial Disc Susceptibility Test. Clinical and Laboratory Standards Institute, 33(1), 59.
Dutka-Malen, S., Evers, S., & Courvalin, P. (1995). Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. Journal of Clinical Microbiology, 33(1), 24-27.
Fisher, K., and Phillips, C. (2009). The ecology, epidemiology and virulence of Enterococcus. Microbiology,155(6), 1749-1757 Gousia, P., Economou, V., Bozidis, P., & Papadopoulou, C. (2015).
Vancomycin-resistance phenotypes, vancomycin-resistance genes, and resistance to antibiotics of enterococci isolated from food of animal origin. Foodborne Pathogens and Disease, 12(3), 214-220.
Kasimoglu-Dogru, A., Gencay, Y.E., & Ayaz, N.D. (2010). Prevalence and antibiotic resistance profiles of Enterococcus species in chicken at slaughter level; absence of vanA and vanB genes in E. faecalis and E. faecium. Research in Veterinary Science, 89(2), 153-158.
Ke, D., Picard, F.J., Martineau, F., Ménard, C., Roy, P.H., Ouellette, M., & Bergeron, M.G. (1999). Development of a PCR assay for rapid detection of enterococci. Journal of Clinical Microbiology, 37(11), 3497-3503.
Kim, Y.B., Seo, H.J., Seo, K.W., Jeon, H.Y., Kim, D.K., Kim, S.W., & Lee, Y.J. (2018). Characteristics of high-level ciprofloxacin-resistant Enterococcus faecalis and Enterococcus faecium from retail chicken meat in Korea. Journal of Food Protection, 81(8), 1357-
Laban, S.E., Mashaly, M.M., Aly, A.M., Maher, N.E., & Zaki, M.M. (2021). Evaluation of different hygienic practices applied in slaughterhouses and its effect on beef quality. Advances in Animal and Veterinary Sciences, 9(3), 429-437.
Manson, A.L., Van Tyne, D., Straub, T.J., Clock, S., Crupain, M., Rangan, U., & Earl, A.M. (2019). Chicken meat-associated enterococci: influence of agricultural antibiotic use and connection to the clinic. Applied and Environmental Microbiology, 85(22),
e01559-19.
Moreno, M. F., Sarantinopoulos, P., Tsakalidou, E., & De Vuyst, L. (2006). The role and application of enterococci in food and health. International Journal of Food Microbiology, 106(1), 1-24.
Naas, H.T., Almajdoubi, Z., Garbaj, A.M., Azwai, S.M., Gammoudi, F.T., Abolghait, S.K., & Eldaghayes, I.M. (2021). Molecular identification and antibiogram of Enterococcus spp. isolated on Enterococcus selective differential (ESD) media from meat, meat products and seafood in Libya. Journal of Microbiology, Biotechnology and Food Sciences, 1264-1268.
Onaran, B., Göncüoğlu, M., & Ormanci, F.S.B. (2019). Antibiotic resistance profiles of vancomycin resistant enterococci in chicken meat samples. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 66(4), 331-336.
Pangtey, R. (2017) Prevalence and Molecular Characterization of Enterococcus faecalis in Bovine Milk. M.V.Sc. thesis submitted to the Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
Poh, C.H., Oh, H.M. L., & Tan, A.L. (2006). Epidemiology and clinical outcome of enterococcal bacteraemia in an acute care hospital. Journal of Infection, 52(5), 383-386.
Rozanska, H., Lewtak-Piłat, A., & Osek, J. (2015). Antimicrobial resistance of Enterococcus faecalis isolated from meat. Bulletin of the Veterinary Institute in Pulawy, 59(2), 229-33.
Sanlibaba P. Tezel B.U., & Senturk E (2018). Antimicrobial resistance of Enterococcus species isolated from chicken in Turkey. Korean Journal for Food Science of Animal Resources, 38(2): 391-402.
Talebi, M., Sadeghi, J., Rahimi, F., & Pourshafie, M.R. (2015). Isolation and biochemical fingerprinting of vancomycin resistant Enterococcus faecium from meat, chicken and cheese. Jundishapur Journal of Microbiology, 8(4).
Zelalem, A., Abegaz, K., Kebede, A., Terefe, Y., & Vipham, J. L. (2022). Investigation on Salmonella enterica, Escherichia coli, and coliforms in beef from Ethiopian abattoirs: A potential risk of meat safety. Food Science & Nutrition, 00, 1– 11.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.