Antimicrobial Resistance and Biofilm Formation in Coagulase Negative Staphylococci Isolated from Bovine Clinical Mastitis

Authors

  • Prakruti J Poshiya Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-360311, Gujarat, India
  • Bhavesh B Javia Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-360311, Gujarat, India
  • Sanjay N Ghodasara Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-360311, Gujarat, India
  • Amit R Bhadaniya Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-360311, Gujarat, India
  • Shobha Khambam Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh-360311, Gujarat, India

DOI:

https://doi.org/10.48165/ijvsbt.20.4.29

Keywords:

Biofilm,, Bovine, Clinical mastitis,, CoNS.

Abstract

Bovine mastitis is a common and economically significant disease that affects dairy cows and buffaloes. Staphylococci are considered  as a most common cause of bovine mastitis. The present research work was undertaken with the objective to isolate and identify  coagulase-negative staphylococci (CoNS) by cultural, biochemical, and molecular methods from bovine mastitic milk samples, and to  study their antimicrobial resistance patterns as well as the biofilm-producing ability. The investigation was carried out on 281 bovine  clinical mastitis milk samples collected in and around Junagadh. Based on cultural and molecular methods, 34.87% (98/281) samples  yielded Staphylococcus isolates. Through the conventional standard tube coagulation test, 16.72% (47/281) isolates were identified as  CoNS. Mannitol fermentative patterns revealed 36.17% (17/47) CoNS isolates as fermentative, while 63.82% (30/47) CoNS isolates as  non-fermentative. CoNS isolates showed high resistance against penicillin-G (64.95%), followed by erythromycin (57.34%), while the  least resistance was observed against oxytetracycline (17.02%). Out of 47 CoNS isolates, 76.59% (36/47) were biofilm producers, while  23.40% (11/47) were non-biofilm producers.

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Published

2024-07-01

Issue

Vol. 20 No. 4 (2024): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) 2024

Section

Research Article

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Copyright (c) 2024 Indian Journal of Veterinary Sciences and Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Poshiya, P.J., Javia, B.B., Ghodasara, S.N., Bhadaniya, A.R., & Khambam, S. (2024). Antimicrobial Resistance and Biofilm Formation in Coagulase Negative Staphylococci Isolated from Bovine Clinical Mastitis . Indian Journal of Veterinary Sciences and Biotechnology, 20(4), 139–142. https://doi.org/10.48165/ijvsbt.20.4.29
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