Molecular Detection and Antibiogram of Bacterial Isolates from Canine Pyoderma Cases
DOI:
https://doi.org/10.48165/ijvsbt.20.4.10Keywords:
Antibiotic susceptibility, Canine pyoderma, Meropenem,, PCR, Penicillin G, Staphylococcus.Abstract
Canine pyoderma is one of the most common causes of dermatitis with worldwide occurrence in small animal practice. This study was carried out to identify bacterial isolates by simplex, multiplex PCR and to know its antibiotic susceptibility profile from canine pyoderma cases. Staphylococcus intermedius (n=17, 53%) was the major species identified followed by Staphylococcus schleiferi (n=10, 32%), Staphylococcus aureus (n=6, 15%), Pseudomonas aeruginosa (n=5, 12.5%) and Escherichia coli (n=3, 7.5%) by PCR. Antibiogram profile of Staphylococcus isolates showed sensitive to enrofloxacin (84.37%), ceftriaxone (81.25%), ceftriaxone/tazobactam (62.5%), whereas P. aueroginosa was sensitive to meropenem (100%), ciprofloxacin (100%), ceftriaxone/tazobactam (80%) and E. coli was sensitive to meropenem (100%), ceftriaxone/tazobactam (100%), ampicillin/sulbactam (100%). All isolates of this study were resistant to pencillin G, clindamycin (P. aueroginosa and E. coli), co-trimoxazole (Staphyloccous spp. & P. aueroginosa) and streptomycin (E. coli) indicating frequent and indiscriminate use of these antimicrobials in pyoderma treatment.
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