Molecular Detection and Antibiogram of Bacterial Isolates from Canine Pyoderma Cases

Authors

  • Chaithanya Milandahalli 1Department of Animal Husbandry and Veterinary Services, Government of Karnataka, India
  • Ramesh Poojary Thimmaiah Department of Veterinary Medicine, Veterinary College, Hebbal, Bengaluru-560024, Karnataka Veterinary, Animal and Fisheries Sciences University, Karnataka, India
  • Chandrashekar Kodavenakapalli Munishamy Department of Veterinary Microbiology, Veterinary College, Gadag-582101, Karnataka Veterinary, Animal and Fisheries Sciences University, Karnataka, India
  • Lathamani Venkatapura Shekharaiah Department of Veterinary Medicine, Veterinary College, Hebbal, Bengaluru-560024, Karnataka Veterinary, Animal and Fisheries Sciences University, Karnataka, India
  • Anjan Kumar Keeramande Ramakrishna Department of Veterinary Pathology, Veterinary College, Hassan-573202 Karnataka Veterinary, Animal and Fisheries Sciences University, Karnataka, India
  • Renukaradhya Gudepalya Jayanna Department of Veterinary Gynaecology and Obstetrics, Veterinary College, Gadag-582101, Karnataka Veterinary, Animal and Fisheries Sciences University, Karnataka, India

DOI:

https://doi.org/10.48165/ijvsbt.20.4.10

Keywords:

Antibiotic susceptibility, Canine pyoderma, Meropenem,, PCR, Penicillin G, Staphylococcus.

Abstract

Canine pyoderma is one of the most common causes of dermatitis with worldwide occurrence in small animal practice. This study was  carried out to identify bacterial isolates by simplex, multiplex PCR and to know its antibiotic susceptibility profile from canine pyoderma  cases. Staphylococcus intermedius (n=17, 53%) was the major species identified followed by Staphylococcus schleiferi (n=10, 32%),  Staphylococcus aureus (n=6, 15%), Pseudomonas aeruginosa (n=5, 12.5%) and Escherichia coli (n=3, 7.5%) by PCR. Antibiogram profile  of Staphylococcus isolates showed sensitive to enrofloxacin (84.37%), ceftriaxone (81.25%), ceftriaxone/tazobactam (62.5%), whereas  P. aueroginosa was sensitive to meropenem (100%), ciprofloxacin (100%), ceftriaxone/tazobactam (80%) and E. coli was sensitive to  meropenem (100%), ceftriaxone/tazobactam (100%), ampicillin/sulbactam (100%). All isolates of this study were resistant to pencillin  G, clindamycin (P. aueroginosa and E. coli), co-trimoxazole (Staphyloccous spp. & P. aueroginosa) and streptomycin (E. coli) indicating  frequent and indiscriminate use of these antimicrobials in pyoderma treatment.

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Published

2024-07-01

Issue

Vol. 20 No. 4 (2024): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) 2024

Section

Research Article

License

Copyright (c) 2024 Indian Journal of Veterinary Sciences and Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Milandahalli, C., Thimmaiah, R.P., Munishamy, C.K., Shekharaiah, L.V., Ramakrishna, A.K.K., & Jayanna, R.G. (2024). Molecular Detection and Antibiogram of Bacterial Isolates from Canine Pyoderma Cases . Indian Journal of Veterinary Sciences and Biotechnology, 20(4), 45–49. https://doi.org/10.48165/ijvsbt.20.4.10
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