Antibiogram Pattern of Bacterial Isolates from Bovine and Bubaline Semen in Karnataka
DOI:
https://doi.org/10.48165/ijvsbt.20.4.06Keywords:
Antibiotics,, Frozen semen, Neat semen, Penicillin, Streptomycin.Abstract
To avert bacterial contamination in semen, antibiotics are added. Inspite of addition of conventional antibiotics, extended and frozen semen samples from bulls have been reported to contain a number of bacterial contaminants which may be due to the antimicrobial resistance because of indiscriminate usage of antibiotics. The present study was conducted to know the antibiotic susceptibility pattern of the bacterial isolates from neat semen and frozen semen straws of cattle and buffalo bulls, collected from three semen stations (Farm A, B & C) of Karnataka. A total of twenty eight Holstein Friesian (HF) bulls, fourteen Murrah and two Surti buffalo bulls were selected for the study. Three time sample collections from each farm at a gap of 15 days were carried out. A total of 205 bacterial isolates were obtained from 264 samples of neat and frozen semen from three different farms (Farm A 49, Farm B 63 and Farm C 93). All isolates were, highly sensitive to newer generation antibiotics Piperacillin-Tazobactam, Imipenem and completely resistant to routinely used antibiotics Penicillin G, and Streptomycin.
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Abro, S.H., Abro, R., Tunio, M., Rind, R., & Bughio, S. (2015). Evidence of bacterial contamination in the frozen bovine semen. Pakistan Journal of Agriculture, Agricultural Engineering and Veterinary Science, 31(1), 102-108.
Ahmed, K., & Greesh, M. (2002). Effect of antibiotics on the bacterial load and quality of semen of Murrah buffalo bulls at different stages of freezing. Indian Journal of Animal Sciences, 72(2), 138-139.
Bauer, A.W., Kirby, W.M.M., Sherris, S.C., & Turk, M. (1966). Antibiotic susceptibility testing by a standardized single disc diffusion method. American Journal of Clinical Pathology, 45(4), 493-496.
CLSI (Clinical and laboratory Standards Institute) (2020). Performance Standards for Antimicrobial Susceptibility Testing, M100S, 30th ED., CLSI Vol.-40, N0. 1, CLSI, Wayne, PA 19087 USA.
Dhruti, Y.P., & Rajesh, K.P. (2012). Estimation of biochemical activities of microbial load isolated from the frozen semen of HF and HF crossbred cattle bulls. Current Trends in Biotechnology and Pharmacology, 6(3), 328-339.
Hasan, S., Andrabi, S.M.H., Muneer, R., Anzar, M., & Ahmad, N. (2001). Effects of a new antibiotic combination on post-thaw motion
characteristics and membrane integrity of buffalo and Sahiwal bull spermatozoa and on the bacteriological quality of their semen. Pakistan Veterinary Journal, 21, 6-12.
Joyjit, M., Sumit, C., Santanu, P., Mrityunjay, C., & Anup, S. (2016). Microbiological evaluation of bovine frozen semen samples in West Bengal, India. Exploratory Animal Medical Research, 6(2), 185-191.
Pankaj, R., & Madhumeet, S, (2020). Prevalence of bacteria in different stages of semen processing before and after semen collection of cow bulls of Himachal Pradesh. Himachal Journal of Agricultural Research, 46(1), 91-94.
Ramaswamy, V., John, K.J., Joseph, A.J.M., Parimal, R., & Venugopalan, A.T. (1994). Prevalence of microbes in frozen cattle semen and their antibiotic spectra. Indian Journal of Animal Reproduction, 15(1), 50-52.
Ramaswamy, V., Latha, N., Gnanasubramanian, T., & Manickam, R. (2002). Aerobic bacteria in buffalo semen and their antibiogram. Indian Journal of Animal Reproduction, 23, 117-119.
Rathnamma, D., Rao, M.S., Ramanatha, K.R., & Raghavan, R. (1997). Assessment of bacterial load in semen of Holstein Friesian bulls. Current Research in University of Agricultural. Sciences, Bangalore, 26(11), 205- 207.
Reda, A.A., Almaw, G., Abreha, S., Tadeg, W., & Tadesse, B. (2020). Bacteriospermia and sperm quality of cryopreserved bull semen used in artificial insemination of cows in South Wollo zone, Ethiopia. Veterinary Medicine International, 202, 1-11.
Ronald, B.S.M., & Prabhakar, T.G. (2001). Bacterial analysis of semen and their antibiogram. Indian Journal of Animal Sciences, 71(9), 829-831.
Ronald, B.S.M., & Prabhakar, T.G. (2002). Effect of Pseudomonas aeruginosa and Proteus mirabilis on spermatozoa. Indian Journal of Animal Sciences, 72(2), 140.62.
Sadeq, J.Z., Kreem, I.A., Ban, N.N., Ahmed, A., Ahmed, S., & Israa, A. (2017). Comparative study of physical properties and bacterial contamination between local and imported straws frozen semen. Journal of Entamology and Zoological Studies, 5(4), 1969-1973.
Sandeep, J., Katoch, R.C., Deepti, C., & Arvind, M. (2000). The drug resistant bacteria associated with frozen semen technology of cow and buffalo bulls. Indian Journal of Animal Sciences, 70, 488-489.
Sharma, R., Pangawkar, G.R., & Matharoo, J.S. (1994.) Efficacy of certain antibiotics on the bacterial load of buffalo semen during cryopreservation. Indian Journal of Animal Sciences, 64, 468-470.
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