Molecular Detection of Babesia motasi in a Goat – First Report from Kerala
DOI:
https://doi.org/10.48165/ijvsbt.20.4.02Keywords:
Babesia,, goat,, Morphometry,, Polymerase chain reaction (PCR), Tick-borne disease.Abstract
Caprine babesiosis is a tick borne haemoprotozoan infection less frequently reported from Kerala. In the present report, we document a confirmed case of Babesia motasi for the first time in the state. A Sirohi Kathiavari crossbred goat was presented to the University Veterinary Hospital (UVH), Kokkalai with symptoms of anorexia and pyrexia. On blood smear examination, large and small forms of Babesia organisms in Giemsa’s stain could be detected. Complete blood count revealed hypochromic microcytic anaemia with thrombocytopenia. Molecular confirmation was done with polymerase chain reaction (PCR) targeting 18S rRNA gene. A 390 bp fragment was obtained and sequenced which was confirmed as Babesia motasi. Babesia ovis or Theileria spp., could not be detected by PCR. This study reports the molecular confirmation of two forms of B. motasi in a crossbred goat from Kerala.
Downloads
References
Ajith, Y., Rajeswari, T.U., Siji, S.R. & Dilip, C. (2017). Case report on babesiosis associated pre-hepatic jaundice in a Malabari goat. Veterinary and Animal Science, 3, 1-3.
Aktas, M., Altay, K. & Dumanli, N. (2005). Development of a polymerase chain reaction method for diagnosis of Babesia ovis infection in sheep and goats. Veterinary Parasitology, 133, 277–281.
Bai, Q., Liu, G., Liu, D., Ren, J. & Li, X. (2002). Isolation and preliminary characterization of a large Babesia spp. from sheep and goats in the eastern part of Gansu Province, China. Parasitology Research, 88, S16– S21.
Bazmani, A., Abolhooshyar, A., Imani-Baran, A. & Akbari, H. (2018). Semi-nested polymerase chain reaction-based detection of Babesia spp. in small ruminants from Northwest of Iran. Veterinary World, 11(3), 268-273.
Christensson, D. & Thunegard, E. (1981). Babesia motasi in sheep on the island of Gotland in Sweden. Veterinary Parasitology, 9, 99–106.
Esmaeilnejad, B., Tavassoli, M., Asri-Rezaei, S., Dalir-Naghadeh, B., Mardani, K., Golabi, M., Arjmand, J., Kazemnia, A. & Jalilzadeh, G. (2015). Determination of prevalence and risk factors of infection with Babesia ovis in small ruminants from West Azerbaijan province, Iran by polymerase chain reaction. Journal of Arthropod-Borne Diseases, 9, 246-252.
Fakhar, M., Hajihasani, A., Maroufi, S., Alizadeh, H., Shirzad, H., Piri, F. & Pagheh, A.S. (2012). An epidemiological survey on bovine and ovine babesiosis in Kurdistan Province, Western Iran. Tropical Animal Health and Production, 44(2), 319–322.
Friedhoff, K.T. (1988). Transmission of Babesia. In: Babesia Infection of Domestic Animals and Man, Ed: M.Ristic, CRC Press, Boca Raton, Florida. Pp 23–52.
Galon, E.M., Zafar, I., Ji, S., Li, H., Ma, Z. & Xuan, X. (2022). Molecular reports of ruminant Babesia in Southeast Asia. Pathogens, 11(8), 915.
Kage, S., Mamatha, G. S., Lakkundi, J. N., Shivashankar, B. P. & D’Souza, P.E. (2019). Detection of incidence of Babesia spp. in sheep and goats by parasitological diagnostic techniques. Journal of Parasitic Diseases, 43, 452-457.
Kaufmann, J. (1996). Text book of Parasitic Infections of Domestic Animals. Birkhauser Verlag, Postfach 133, CH-4010 Basel, Schweiz. pp 169–170.
Lewis, D., Holman, M.R. & Purnell, R.E. (1981). Investigations on Babesia motasi isolated from Wales. Research in Veterinary Science, 31, 239-243.
Muthuramalingam, T., Pothiappan, P., Gnanaraj, P.T., Sundaram, S.M., Pugazhenthi, T.R. & Parthiban, S. (2014). Report on an outbreak of babesiosis in Tellicherry goats. Indian Journal of Veterinary and Animal Science Research, 43, 58-60.
Papadopoulos, B., Brossard, M. & Perie, N.M. (1996). Piroplasms of domestic animals in the Macedonia region of Greece. Piroplasms of small ruminants. Veterinary Parasitology, 63(1–2), 67–74.
Razmi, G., Naghibi, A., Aslani, M., Dastjerdi, K. and Hossieni, H. (2003) An epidemiological study on Babesia infection in small ruminants in Mashhad suburb, Khorasan province, Iran. Small Ruminant Research, 50, 39-44.
Sevinc, F., Sevinc, M., Ekici, O.D., Yildiz, R., Isik, N. & Aydogdu, U. (2013). Babesia ovis infections: detailed clinical and laboratory observations in the pre and post treatment periods of 97 field cases. Veterinary Parasitology, 191, 35-43.
Shayan, P. & Rahbari, S. (2005). Simultaneous differentiation between Theileria spp. and Babesia spp. on stained blood smear using PCR. Parasitology Research, 97, 281-286.
Shayan, P., Hooshmand, E., Nabian, S. & Rahbari, S. (2008). Biometrical and genetical characterization of large Babesia ovis in Iran. Parasitology Research, 103, 217-221.
Soulsby, E.J.L. (1982). Helminths, Arthropods and Protozoa of Domesticated Animals. 7th edn. BailliereTindall, London, UK., PP 706- 728.
Taylor, M.A., Coop, R.L. & Wall, R.L. (2007). Veterinary Parasitology. 3rd edn., Oxford, Blackwell Publishing. PP 553-555. Yin, H., Schnittger, L., Luo, J., Seitzer, U., & Ahmed, J.S. (2007). Ovine theileriosis in China: a new look at an old story. Parasitology Research, 101(2), 191–195.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Indian Journal of Veterinary Sciences and Biotechnology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
