Cultural and Biochemical Characterization of Staphylococcus aureus Isolates from Bovine Clinical and Subclinical Mastitis

Authors

  • Pallavi Maddela Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Navsari-396450, Gujarat, India
  • Pushpa M Makwana Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Navsari-396450, Gujarat, India
  • Saurabh M Parmar Department of Veterinary Clinical Complex, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Navsari-396450, Gujarat, India
  • Irsadullahkhan H Kalyani Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Navsari-396450, Gujarat, India
  • Dharmesh R Patel Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Navsari-396450, Gujarat, India
  • Dixit K Parasana Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Navsari-396450, Gujarat, India

DOI:

https://doi.org/10.48165/ijvsbt.20.3.29

Keywords:

CMT, Mannitol fermentation, Mastitis, Slime production, Staphylococcus aureus

Abstract

Bovine mastitis causes huge economic losses to the dairy industry all over the world. Staphylococcus aureus is a most common causative  agent of bovine mastitis. The study was undertaken with an objective of isolation, identification and biochemical characterization of  Staphylococcus aureus isolated from bovine mastitis cases. Total of 256 bovine milk samples (32 from clinical mastitis and 224 from  suspected cases of subclinical mastitis) were collected from in and around Navsari. Of the 224 SCM suspected milk samples screened  by California mastitis test (CMT), 108 (48.21 %) samples were found positive for subclinical mastitis, whereas all 32 clinical mastitis  samples were CMT positive. Thus total 140 bovine milk samples (32 clinical and 108 subclinical mastitis) were used for detection of  Staphylococcus aureus. Out of these, 17 (12.14%) isolates were identified as Staphylococcus aureus on the basis of cultural characteristics  and biochemical tests. From 17 S. aureus, 16 (94.11%) isolates were categorized as mannitol fermenter and coagulase positive, while  1 (5.88%) isolate was mannitol non-fermenter and coagulase negative. Majority of the isolates 10 (58.82%) exhibited beta-haemolysis,  whereas 5 (29.41%) isolates showed alpha-haemolysis and 2 (11.76%) isolates were non-haemolytic on 5% sheep blood agar. Among  17 S. aureus isolates, 14 (82.35%) isolates exhibited slime production, while 6 (11.76%) isolates were negative for slime production. This  study showed overall 12.14 % incidence of bovine mastitis by Staphylococcus aureus, whereas cases of clinical mastitis and sub-clinical  mastitis noted as 9.30% and 12.96%, respectively, by S. aureus. 

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Published

2024-05-10

Issue

Vol. 20 No. 3 (2024): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) 2024

Section

Research Article

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Copyright (c) 2024 Indian Journal of Veterinary Sciences and Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Maddela, P., Makwana, P.M., Parmar, S.M., Kalyani, I.H., Patel, D.R., & Parasana, D.K. (2024). Cultural and Biochemical Characterization of Staphylococcus aureus Isolates from Bovine Clinical and Subclinical Mastitis. Indian Journal of Veterinary Sciences and Biotechnology, 20(3), 149–152. https://doi.org/10.48165/ijvsbt.20.3.29
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