In Vitro Efficacy of Curcuma longa in Combating Staphylococcus aureus Subclinical Mastitis in Buffalo

Authors

  • Ankur Singh Department of Veterinary Medicine, College of Veterinary Science & AH, Acharya Narendra Deva University of Agriculture & Technology, Kumarganj, Ayodhya, Uttar Pradesh, India-224229
  • Satyavrat Singh Department of Veterinary Medicine, College of Veterinary Science & AH, Acharya Narendra Deva University of Agriculture & Technology, Kumarganj, Ayodhya, Uttar Pradesh, India-224229
  • Jitendra Pratap Singh Department of Veterinary Medicine, College of Veterinary Science & AH, Acharya Narendra Deva University of Agriculture & Technology, Kumarganj, Ayodhya, Uttar Pradesh, India-224229
  • Rajesh Kumar Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & AH, Acharya Narendra Deva University of Agriculture & Technology, Kumarganj, Ayodhya, Uttar Pradesh, India-224229
  • Naveen Kumar Singh Veterinary Clinical Complex, College of Veterinary Science & AH, Acharya Narendra Deva University of Agriculture & Technology, Kumarganj, Ayodhya, Uttar Pradesh, India-224229

DOI:

https://doi.org/10.48165/ijvsbt.20.2.23

Keywords:

Buffalo, Curcuma longa, Subclinical mastitis

Abstract

Subclinical mastitis (SCM) being hidden, goes unnoticed until diagnosed in the lab and is therefore the most studied type of mastitis engaging  researchers to look for efficient therapeutic or preventive modalities. The in-vitro efficacy of aqueous, methanolic and hydro-methanolic extracts  of Curcuma longa was studied against isolates of S. aureus from SCM cases of buffaloes. The stock solution at the concentrations of 50 (HM-50),  100 (HM-100), 150 (HM-150), 200 (HM-200) and 250 (HM-250) mg/mL was used to prepare the disc. Enrofloxacin disc was used as standard. The  highest zone of inhibition (8.2 mm, and 10.1 mm) was noted with the concentration 250 mg/disc of aqueous and methanolic extracts of Curcuma  longa, respectively. The hydro-methanolic extract failed to yield zone of inhibition against S. aureus at all five concentrations. It can be concluded  that the antimicrobial potential of aqueous and methanolic extract of Curcuma longa can be used in the treatment or prevention of mastitis.  

Downloads

Download data is not yet available.

References

Bauer, A.W., Kirby, W.M., Sherries, J.C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45, 493-496.

Bukar, A., Uba, A., & Oyeyi, T.I. (2010). Phytochemical analysis and antimicrobial activity of Parkia biglobosa (Jacq.) Benth extracts against some food-borne microorganisms. Advances in Experimental Biology 4(1), 74-79.

Cheppudira, B., Fowler, M., McGhee, L., Greer, A., Mares, A., Petz, L., Devore, D., Loyd, D.R., & Clifford, J.L. (2013). Curcumin: A novel therapeutic for burn pain and wound healing. Expert Opinion on Investigational Drugs, 22(10), 1295-1303.

Halasa, T., Huijps, K., Osteras, O., & Hogeveen, H. (2007). Economic effects of bovine mastitis and mastitis management: A review. Vet. Quarterly, 291, 18-31.

Hegde, M.N., Shetty, S., Yelapure, M., & Patil, A. (2012). Evaluation of antimicrobial activity of aqueous and hydro-alcoholic Curcuma longa extracts against Endodontic Pathogens. IOSR Journal of Pharmacy, 2(2), 192-198.

Keskin, D., & Ceyhan-Guvensen, N. (2019). Antimicrobial activity and chemical constituent of different extracts of rhizome of termeric (Curcuma longa) from West Anatolia. Fresenius Environmental Bulletin, 28(3), 1652-1656.

Lorian, V. (1996). Antibiotics in Laboratory Medicine. 4th edition, Williams and Wilkins, Baltimore, London, ISBN 9780781749831 Moghadamtousi, S.Z., Kadir, H.A., Hassandarvish, P., Tajik, H., Abubakar, S., & Zandi, K. (2014). A review on antibacterial, antiviral, and antifungal activity of curcumin. BioMed Research International, Article ID 186864, 12 pages.

Mungube, E.D., Tenghagen, B.A., Regassa, F., Kyule, M.N., Shiferaw, Y., Kassa, T., & Baumann, M.P.O. (2005). Reduced milk production in udder quarters with subclinical mastitis and associated economic losses in crossbred dairy cows in Ethiopia. Tropical Animal Health Production, 37(6), 503-512.

Napolean, P., Anitha, J., & Emilin, R.R. (2009). Isolation, analysis and identification of phytochemicals of antimicrobial activity of Moringa oleifera Lam. Current Biotica, 3(1), 33-37.

Niamsa, N., & Sittiwet, C. (2009). Antimicrobial activity of Curcuma longa aqueous extract. Journal of Pharmacology and Toxicology, 4(4), 173-177.

Ogbonna, C., & Ozgor, E. (2021). Detection of biological activity of Curcuma longa methanol extract and its antibacterial effect on Staphylococcus aureus. The Euro-Biotech Journal, 5(2), 56-62.

Raina, K.M., Mohamed, A., Bayoumi, Salah, F.A., & Abd, E. (2013). MRSA detection in raw milk, some dairy products and hands of dairy workers in Egypt, a mini-survey. Food Control, l33(1), 49-53.

Rainard, P., Foucras, G., & Fitzgerald, J.R. (2018). Knowledge gaps and research priorities in Staphylococcus aureus mastitis control. Transbound Emerging Disease, 65(Suppl 1), 149-165.

Samie, A., Green, E., Obi, C.L., Bessong, P.O., & Ndip, R.N. (2010). Inhibitory properties of selected South African medicinal plants against Mycobacterium tuberculosis. Journal of Ethnopharmacology, 130(1), 151-157.

Schroeder, J. (2010). Mastitis Control Programs: Bovine Mastitis and Milking Management. North Dakota State University Extension Bulletin, as-1129.

Singh, M., Rai, R.B., Dhama, K., Saminathan, M., Tiwari, R., Chakraborty, S., Damodaran, T., Malik, Y.P.S., & Singh, B. (2013). Bovine Mastitis: The disease diagnosis, prevention, treatment and control. Indian Veterinary Research Institute, Izatnagar, Bareilly, India, Bulletin, pp. 1-44.

Tyagi, P., Singh, M., Kumari, H. Kumari, A., & Mukhopadhyay, K. (2015). Bactericidal activity of curcumin I is associated with damaging of bacterial membrane. Plos one, 10(3), 1-15.

Vasudevan, P., Nair, M.K.M., Annamalai, T., & Venkitanarayanan, K.S. (2003). Phenotypic and genotypic characterization of bovine mastitis isolates of Staphylococcus aureus for biofilm formation. Veterinary Microbiology, 92, 179-185.

Zaiden, M.R., Noor Rain. A., Badrul, A.R., Adlin, A., Norazah, A., & Zakiah, I. (2005). In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method. Tropical Biomedicine, 22, 165-170.

Published

2024-03-10

How to Cite

Singh, A., Singh, S., Singh, J.P., Kumar, R., & Singh, N.K. (2024). In Vitro Efficacy of Curcuma longa in Combating Staphylococcus aureus Subclinical Mastitis in Buffalo. Indian Journal of Veterinary Sciences and Biotechnology, 20(2), 107–110. https://doi.org/10.48165/ijvsbt.20.2.23