PCR BASED DETECTION OF COAGULASE GENE ENCODING STAPHYLOCOCCUS AUREUS ISOLATED FROM SUBCLINICAL MASTITIS CASES IN BUFFALOES

Authors

  • S Malmarugan Department of Veterinary Microbiology Veterinary College and Research Institute, Namakkal, (T.N.)
  • M Arthanarieswaran Department of Veterinary Microbiology Veterinary College and Research Institute, Namakkal, (T.N.)
  • P Suresh Department of Veterinary Microbiology Veterinary College and Research Institute, Namakkal, (T.N.)

DOI:

https://doi.org/10.48165/

Keywords:

Buffaloes, Staphylococcus aureus, sub-clinical mastitis, coagulase gene

Abstract

The present study was carried out to characterize Staphylococcus aureus (S. aureus) isolated from mastitic cases in buffalo based on coagulase gene. A total of 18 strains of S. aureus were isolated during processing of 81 milk samples from 21 buffaloes. The S. aureus strains were characterized phenotypically, and were further characterized genotypically by polymerase chain reaction using oligonucleotide primers that amplified genes encoding coagulase (Coa). Amplification of the Coa gene yielded a product size of 730bp in all 15 coagulase positive isolates. The phenotypic and genotypic findings of the present strategies might provide an understanding of the distribution of the cogulase positive S. aureus clones among buffaloes mastitis isolates, and might aid in the development of steps to control S. aureus infections in buffaloes. 

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References

Aslantas, O., Demir, C and Turutoglu. H (2007). J; Vet. Anim. Sci 31:253-257

Coelho, S.M. O., Reinoso, E., Pereira, I.A., Soares, L.C., Demo, M., Bogni, C and Souza, M.M. S. (2009). Pesq. Vet. Bras. 29 (5) : 369-374

Cowan S.T and Steel K.J. (1970). Manual for the Identification of Medical Bacteria, pp. 52-57, Cambridge University Press, London.

Cruickshank R, Duguid I. P, Harmoin BP and Swain RHA (eds). (1975). The practice of medical microbiology. In: Medical Microbilogy. Vol. 2. 12th ed. pp. 356-366, Churchill Livingstone, Edinburgh.

Fitzgerald JR, Meaney WJ, Hartigan PJ, Smyth CJ, Kapur V. (1997) Epidemiol Infect 119: 261-269. Fitzgerald JR, Hartigan P.I, Meaney W.J and Smyth C.J (2000). J Appl Microbiol , 88, 1028-1037. Foster G, Ross HM, Hutson RA, Collins MD. (1997.) Int J Syst Bacteriol 47, 724-726. Foster SJ. ( 1995 ). J Bacteriol 177, 5723-5725.

Kalorey, D.R., Shanmugam, Y., Kurkure, N.V., Chousalkar, K.K. and Barbuddhe, S.B.( 2007).J. Vet. Sci. 8(2), 151-154.

Kapur V, Sischo WM, Greer RS, Whittam TS, Musser JM. 1995. J Clin Microbiol (1995) , 33, 376-380. Lachica RVF, Genigeorgis C, Hoeprich PD. (1971). Appl Microbiol , 21, 585-587. Miles H, Lesser W, Sears P. (1992). J Dairy Sci, 75. 596-6Q5.

Radostits OM, Gay CC, Blood DC, Hinchclifl KW.( 2003). Veterinary Medicine. A Textbook of the Diseases of Cattle, Sheep, Pigs. Goats and Horses. 9th ed. p. 615, Saunders, Philadelphia.

Salasia SIO., Khusnan, Z., Lammler, C and Zschock, M.( 2004). J. Vet. Sci. 5, 103-109.

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Published

2010-06-29

Issue

Vol. 5 No. 3 (2010): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (2010)

Section

Articles

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Copyright (c) 2024 Indian Journal of Veterinary Sciences & Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Malmarugan, S., Arthanarieswaran, M., & Suresh, P. (2010). PCR BASED DETECTION OF COAGULASE GENE ENCODING STAPHYLOCOCCUS AUREUS ISOLATED FROM SUBCLINICAL MASTITIS CASES IN BUFFALOES . Indian Journal of Veterinary Sciences and Biotechnology, 5(3), 53–54. https://doi.org/10.48165/
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