Meat Species Identification in Chicken Blood Meal using  Polymerase Chain Reaction Technique

K SUDHEER; R NARENDRA BABU; V APPA RAO; A SINDHURA; S RAZIUDDIN; M.  KALYANA CHAKRAVARTHI

doi:10.48165/ijapm.2023.37.2.11

Authors

K SUDHEER Dept. of LPT, College of Veterinary Science, Tirupati, 517502, SVVU, Andhra Pradesh
R NARENDRA BABU Dept. of LPT, (Meat Science), Madras Veterinary College, TANUVAS, Chennai
V APPA RAO Directorate of Extension Education, Tamil Nadu Veterinary and Animal Sciences University, Chennai
A SINDHURA . Dept. of LPT, (Meat Science), Madras Veterinary College, TANUVAS, Chennai
S RAZIUDDIN Dept. of LPT, (Meat Science), Madras Veterinary College, TANUVAS, Chennai
M. KALYANA CHAKRAVARTHI Directorate of Extension Education, Tamil Nadu Veterinary and Animal Sciences University, Chennai

DOI:

https://doi.org/10.48165/ijapm.2023.37.2.11

Keywords:

cyt b gene, chicken, blood meal, polymerase chain reaction

Abstract

A study was conducted to identify the species of chicken in high heat processed chicken secondary by-product comprising of poultry blood meal. A total of 30 samples of poultry blood meal were collected from different poultry processing plants in Tamil Nadu. Species specific polymerase chain reaction was employed to validate the meat species identification in heat processed secondary poultry by-products. Custom designed primer sequences of mitochondrial cyt b gene were utilised for the present study. The results revealed successful amplification of chicken species at 227 base pairs in samples of all the blood meal samples. Thus, it was concluded that species specific polymerase chain reaction technique can be effectively used in the screening of chicken meat species in different heat processed secondary by-products for quick and reliable results.

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