Histopathological Features of Myosarcoma in the Thigh Muscle of Cattle after Surgical excisions
DOI:
https://doi.org/10.48165/ijapm.2026.42.SI.3Keywords:
Myosarcoma, Cattle, Thigh muscle, Histopathology, Neoplasm , Skeletal muscle tumors.Abstract
The aim of the study was to present the clinical conditions, operative approach and the histopathological appearances of a malignant skeletal muscle tumour (myosarcoma) in the thigh of an adult cow. It was done to the animal by slowly increasing a mass more than six months, which resulted in mild lameness, diminished weight-bearing of the affected limb, and disturbed normal locomotion. The tumor was excised surgically in aseptic conditions to alleviate functional impairment and to give tissues to make the definitive diagnosis. Representative tissue samples were fixed in 10% neutral buffered formalin, processed initially, embedded in paraffin, sectioned at 45 -5 0.1 and stained with hematoxylin and eosin to be examined under the microscope. Histopathological examination allowed observing a massive invasion of nor mal skeletal muscle fibers with hyperchromatic and irregular nuclei, highly pleomorphic neoplastic cells, which are replaced, with a poorly defined cy toplasm, and without normal striations. Other observations were the evi dence of significant vascular congestion, inflammatory cell infiltration, large zones of coagulative necrosis, and angiogenesis. These characteristics were also similar in all the analyzed sections and suggested aggressive malignant behavior. This research establishes the malignancy of the tumor as being of high grade and the importance of a histopathological study in the achievement of a proper diagnosis. Surgical excision with the help of a microscopic examination not only makes it easy to diagnose but also enhance the clinical management by eliminating the mechanical barrier and dysfunctional effect of the tumor. The results are important in understanding the biological behaviour of rare skeletal muscle tumours in cattle, which increases the knowledge of recognition, diagnosis and treatment plans in veterinary practice.
References
Abduljaleel, M. R., Abdulrazaq, A. W., Jassim, M. M., Abbas, M. F., Alfaris, A. A., Naeem, R. M., Ibrahim, A. A., Mohammed Saleh, W. M., Alrashid, I. M., & Alrafas, H. R. (2025). Understanding the synergistic impact of atropine with xylazine and ketamine on recovery time, heart rate, and respiratory rate in male rabbits. Assiut Veterinary Medical Journal, 71(187), 237–246. https://doi.org/10.21608/avmj.2025.341413.1505
Bancroft, J. D., & Gamble, M. (2013). Theory and practice of histological techniques (7th ed.). Churchill Livingstone.
Cullen, J. M., Page, R., & Misdorp, W. (2014). Mesenchymal tumors of domestic animals. Veterinary Pathology, 51(1), 198–215. https://doi.org/10.1177/0300985813505130
Dolmans-Vasconcelos, J., Pires, M. A., Alves, A., Vieira Pinto, M., Saraiva, C., & Cardoso, L. (2023). Neoplasms in domestic ruminants and swine: A systematic literature review. Veterinary Sciences, 10(2), 163. https://doi.org/10.3390/vetsci10020163
Goldschmidt, M. H., & Hendrick, M. J. (2002). Tumors of the skin and soft tissues. Armed Forces Institute of Pathology.
Hogendoorn, M. P., van der Luer, R. J. T., & van den Ingh, T. S. G. A. M. (2008). Paratesticular sclerosing rhabdomyosarcoma in a testicle of a Nubian goat. Tijdschrift voor Diergeneeskunde, 133(20), 850–852.
Ibrahim, A. A., Naeem, R. M., Abduljaleel, M. R., Jassim, M. M., Abdulrazaq, A. W., Khaleefah, I. A., & Hashim, A. M. (2025). Effect of platelet-rich fibrin (PRF) from different sources on avulsion wound healing in a rabbit model. Journal of Animal Health and Production, 13(4), 1244–1251. https://doi.org/10.17582/journal.jahp/2025/13.4.1244.1251
Jasim, M. M., Naeem, R. M., Abduljaleel, M. R., Sanad, N. H., Ibrahim, A. A., & Alrafas, H. R. (2025). Efficacy of autogenic, allogenic, and heterogenic platelet-rich plasma (PRP) on avulsion skin wounds in rabbit model. Advances in Life Sciences, 12(1), 91–97. https://doi.org/10.62940/als.v12i1.2907
Maxie, M. G. (2016). Jubb, Kennedy, and Palmer’s pathology of domestic animals (6th ed.). Elsevier.
Meuten, D. J. (2017). Tumors in domestic animals (5th ed.). Wiley-Blackwell. https://doi.org/10.1002/9781119181200
Mohsin, T. A., Abduljaleel, M. R., Radhi, A. J., Abbas, M. F., Alrashid, I. M. H., & Khudhair, Z. W. (2025). Histopathological effects of streptomycin treatment on macrophages in lymph nodes, spleen, liver, and kidneys of rats. Advances in Animal and Veterinary Sciences, 13(6), 1337–1345. https://doi.org/10.17582/journal.aavs/2025/13.6.1337.1345
Moulton, J. E. (1990). Tumors in domestic animals. University of California Press.
Radostits, O. M., Gay, C. C., Hinchcliff, K. W., & Constable, P. D. (2007). Veterinary medicine: A textbook of the diseases of cattle, horses, sheep, pigs, and goats (10th ed.). Saunders Elsevier.
Thompson, K. G. (2007). Skeletal muscle tumors in domestic animals. Veterinary Pathology, 44(1), 1–17. https://doi.org/10.1354/vp.44-1-1
Valli, V. E. (2007). Classification of soft tissue tumors in domestic animals. Veterinary Clinical Pathology, 36(1), 21–25. https://doi.org/10.1111/j.1939-165X.2007.tb00181.x
Zachary, J. F. (2017). Pathologic basis of veterinary disease (6th ed.). Elsevier.
