Efficacy of Hormonal Injection on Day 11 Post-Mating in Reducing Embryonic Loss and Selected Blood Parameters in Awassi Ewes
DOI:
https://doi.org/10.48165/ijapm.2026.42.1.14Keywords:
Kisspeptin-10; GnRH; Hormones; Embryonic Loss ; Awassi ewesAbstract
This study aimed to evaluate the effects of a single hormonal injection of Kisspeptin-10 ,GnRH ,hCG and progesterone administered on day 11 post-mating on embryonic survival, serum progesterone concentration, selected amino acid and fatty acid profiles, and reproductive performance in Awassi ewes. Fifty Awassi ewes (3–4 years old; 40–45 kg body weight) were synchronized using intravaginal sponges containing 20 mg flugestone acetate for 14 days and naturally mated. Ewes were randomly allocated into five groups :first group/ control (2 mL normal saline), second group / Kisspeptin-10 (10 µg/kg BW), third group /GnRH (2.5 mL), fourth group /hCG (0.5 mL), and fifth group / progesterone (0.5 mL), administered on day 11 post-mating. Blood samples were collected on days 34, 51, and 68 of gestation. Results showed significant increase (P<0.05)in progesterone and cholesterol of hormonal treatment on day 51,68 compared to control group. Hormonal treatments significantly increased serum amino acid and fatty acid concentrations (P < 0.01) compared with control. Hormonal treatments improved reproductive performance , fertility, twinning rate compared with control . In conclusion, hormonal administration on day 11 post-mating enhances luteal function ,and may contribute to reduced embryonic loss and improved reproductive efficiency in Awassi ewes.References
Abadi, F. M., Mirfazeli, A., Zaeri, H., Nejabat, M., Taherizadeh, M., Ariaie, M., & Joshaghani, H. (2016). Analysis of plasma amino acids using RP-HPLC and pre-column derivatization with OPA/3-MPA. Medical Laboratory Journal, 10(2).
Abecia, J. A., Forcada, F., Palacín, I., & Beckers, J. F. (2020). Hormonal control of reproduction and metabolism in sheep. Animals, 10(1), 70. https://doi.org/10.3390/ani10010070
Allain, C. C., Poon, L. S., Richmond, C. S. G., & Fu, P. C. (1974). Enzymatic determination of total serum cholesterol. Clinical Chemistry, 20, 470–475.
Al-Qass, J. E., Daib, I. A., & Al-Jalili, Z. F. (1993). Fundamentals of sheep and goat production and management. Dar Al-Hikma Press, University of Baghdad.
Antoniazzi, A. Q., Webb, B. T., Romero, J. J., Ashley, R. L., Smirnova, N. P., Henkes, L. E., Bott, R. C., & Hansen, T. R. (2013). Endocrine delivery of interferon tau protects the corpus luteum from prostaglandin F2α-induced luteolysis in ewes. Biology of Reproduction, 88(6), Article 144. https://doi.org/10.1095/biolreprod.112.105684
Bazer, F. W., Ott, T. L., & Spencer, T. E. (1998). Maternal recognition of pregnancy: Comparative aspects: A review. Placenta, 19(Suppl. 2), 375–386.
Bell, A. W., & Greenwood, P. L. (2016). Nutrient partitioning and metabolic adaptations during pregnancy in ruminants. Journal of Animal Science, 94, 185–197.
Beltramo, M., Robert, V., Decourt, C., & Delaunay, F. (2018). Towards new strategies to manage livestock reproduction using kisspeptin analogs. Theriogenology, 112, 148–155. https://doi.org/10.1016/j.theriogenology.2018.01.037
Christenson, L. K., & Devoto, L. (2003). Cholesterol transport and steroidogenesis by the corpus luteum. Reproductive Biology and Endocrinology, 1, 90. https://doi.org/10.1186/1477-7827-1-90
Coleson, M. P., Sanchez, N. S., Ashley, A. K., Ross, T. T., & Ashley, R. L. (2015). Human chorionic gonadotropin increases serum progesterone, number of corpora lutea and angiogenic factors in pregnant sheep. Reproduction, 150(1), 43–52. https://doi.org/10.1530/REP-14-0632
Coleson, M. P., Sanchez, N. S., Ashley, A. K., Ross, T. T., & Ashley, R. L. (2015). Human chorionic gonadotropin increases serum progesterone, number of corpora lutea and angiogenic factors in pregnant sheep. Reproduction, 150(1), 43–52. https://doi.org/10.1530/REP-15-0103
Cunha, T. O., & Martins, J. P. N. (2022). Graduate student literature review: Effects of human chorionic gonadotropin on follicular and luteal dynamics and fertility in cattle. Journal of Dairy Science, 105, 8401–8410. https://doi.org/10.3168/jds.2022-21991
da Fonseca, J. F., Castro, A. C. R., Arashiro, E. K. N., & colleagues. (2018). Effects of hCG administration on accessory corpus luteum formation and progesterone production in estrous-induced nulliparous Santa Inês ewes. Animal Reproduction, 15(2), 135–139.
Diskin, M. G., & Kenny, D. A. (2019). Optimising reproductive performance of beef and dairy cattle and sheep. Animal.
Duncan, D. B. (1955). Multiple range and multiple F-test. Biometrics, 11, 1–24.
Edwards, S. J., Smaill, B., O’Connell, A. R., Johnstone, P. D., Stevens, D. R., Quirke, L. D., & Juengel, J. L. (2016). Reduced ovulation rate, failure to be mated and fertilization failure/embryo loss are the underlying causes of poor reproductive performance in juvenile ewes. Animal Reproduction Science, 167, 125–132. https://doi.org/10.1016/j.anireprosci.2016.02.017
Fernandez, J., Bruno-Galarraga, M. M., Soto, A. T., Cueto, M. I., Gibbons, A. E., & Gonzalez-Bulnes, A. (2018). Hormonal therapeutic strategy on the induction of accessory corpora lutea in relation to follicle size and on the increase of progesterone in sheep. Theriogenology, 105, 184–188. https://doi.org/10.1016/j.theriogenology.2017.09.030
Fernández, J., Bruno-Galarraga, M., Cueto, M. I., Bonadeo, N., Notaro, U., Soto, A. T., et al. (2024). Changes on corpus luteum structure and progesterone synthesis pathway after hCG or GnRH treatment during the early luteal phase in sheep. Animal Reproduction Science, 265, 107474. https://doi.org/10.1016/j.anireprosci.2024.107474
Gottsch, M. L., Clifton, D. K., & Steiner, R. A. (2009). From KISS1 to kisspeptins: An historical perspective and suggested nomenclature. Peptides, 30(1), 4–9. https://doi.org/10.1016/j.peptides.2008.06.016
Hitch, C. J., & Kansal, R. (2022). Kisspeptin and its potential as a reproductive therapeutic in domestic animals. Animals, 12(3), 345. https://doi.org/10.3390/ani12030345
