Gene Pyramiding: a Strategy for Durable Crop Protection in Vegetable Crops

Journal of Agriculture and Allied fields
Year: (2021), Volume: (3), Issue. (1)
First page: (28) Last page: (36)
Online ISSN: A/F

Gene Pyramiding: a Strategy for Durable Crop Protection in Vegetable Crops


Senior Research Fellow, CSK HPKV Palampur, Distt. Kangra (Himachal Pradesh) Pin Code: 176061

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Online Published: 10-July-2021

28th February 2021

21st June 2021

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Gene pyramiding technique can be termed as a boon in breeding methodologies as it used in improving existing elite cultivars for a few unsatisfactory traits. It is defined as a method aimed at assembling multiple genes with known effects on target traits. It works on the objective to enhance trait performance through best utilization of source diversity by introgression; this broadens genetic base of the. cultivars. Durable resistance identification, introgression and confirmation for gene pyramiding is extremely difficult to achieve using conventional methods as it involves extensive phenotyping, linkage drag, pleiotropism and time consuming (Malav et al., 2016). Marker-based selection acts an effective, efficient and economic alternative to avoid the above mentioned (Ye and Smith, 2008). Major work has been done in vegetables using marker assisted gene pyramiding. By this technique resistance genes for PMMoV, TSWV and PVY were genetically combined in one superior pepper line by pyramiding via marker assisted backcross selection and resistance assays (Ozkaynak et al., 2014). Though high cost of marker construction, sequencing sounds as a limitation for their utilization, the accuracy, short time required for development of a variety, easy genetic confirmation of traits makes it the most suitable method for employment in gene pyramiding. The combined utilization of advanced biotechnological tools for combining favourable traits into a single hybrid would avoid glut of new booming varieties in the market thus benefiting farming sector.


introgression,phenotyping, linkage drag, pleiotropism,PMMoV, TSWV and PVY