Evaluation of mineral mixture, Probiotics, enzymes and acidifiers effect on serum biochemistry of broiler chickens
DOI:
https://doi.org/10.48165/jvls.2025.1.1.6Keywords:
Feed additives, Serum biochemistry Probiotics, Enzymes, Organic minerals, AcidifiersAbstract
The present study was conducted to evaluate the effects of dietary supplementation with organic mineral mixtures, probiotics, enzymes, and acidifiers on the serum biochemical parameters of broiler chickens. A total of 150 day-old straight-run VenCobb broiler chicks were randomly assigned to five treatment groups (T0–T4) with three replicates of 10 chicks each. The groups included a control (T0) receiving only a basal diet, while T1 to T4 were supplemented with organic mineral mixture (0.50 g/kg), probiotics (1.0 g/kg), enzymes (0.50 g/kg), and acidifiers (1.0 g/kg), respectively. The birds were reared under uniform management conditions for 42 days. At the end of the trial, serum samples were analyzed for glucose, total cholesterol, triglycerides, urea, creatinine, and BUN/creatinine ratio using commercial diagnostic kits. The results revealed a significant reduction (p ≤ 0.05) in serum glucose, cholesterol, and triglyceride levels in all supplemented groups compared to the control. The enzyme-supplemented group (T3) exhibited the most pronounced reduction in lipid parameters. Urea and creatinine concentrations were significantly lower in the supplemented groups, with the lowest creatinine observed in T3, indicating improved protein metabolism and renal function. The BUN/creatinine ratio was also significantly reduced in all treatment groups, reflecting enhanced nitrogen utilization. These findings suggest that supplementation with organic minerals, probiotics, enzymes, and acidifiers positively modulate the serum biochemical profile of broiler chickens, indicating improved metabolic efficiency and physiological health.
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