Extraction of natural food color from Celosia cristata using orbital shaking apparatus
Keywords:
Betalin, cockscomb, orbital shaking apparatus, temperature, solventAbstract
As there is a growing demand for eco-friendly and non-toxic food colorants, the colors obtained from plant sources have proven to be an alternative to the synthetic food colors. In the present study, natural pigment and color was extracted from the flowers of Celosia cristata using orbital shaking apparatus. Three different temperatures (20, 40, and 60 oC) and three different solvents (distilled water, 30% ethanol, 50% ethanol) were used for the extraction. Optimum extraction conditions for maximizing the total betalin content (17.67 mg/100g), antioxidant activity (38.54%) and colorant yield (23.98%) were temperature of 40 oC using distilled water for an extraction period of 2 hours. Betalin content decreased at higher temperature (60 oC) of extraction that depicts the thermal degradation of the betalin content. The solid liquid ratio taken for extraction was 1:10 (w/v).
References
Boo, H.O., Hwang, S. J., Bae, C.S., Park, S. H. and Song, W.S. 2011. Antioxidant activity according to each kind of natural plant pigments. Korean Journal of Plant Resources, 24:134-141.
Masar, M., Kaniansky, D. and Madajová, V. 1996. Separation of synthetic food colorants by capillary zone electrophoresis in a hydrodynamically closed separation compartment. Journal of Chromatography A, 724: 327–336. Maran, J. P., Sivakumar, V., Thirugnanasambandham, K. and Sridhar, R. 2014. Extraction of natural anthocyanin and colors from pulp of jamun fruit. Journal of Food Science and Technology, 52(6): 3617-3626.
Taha, R.M. and Wafa, S. N. 2012. Plant regeneration and cellular behavior studies in Celosia cristata grown In vivo and In vivo. The scientific World Journal, 2012: 1-8.
Cai, Y., Sun, M., Schliemann, W. and Corke, H. 2001. Chemical stability and colorant properties of betaxanthin pigments from Celosia argentea. Journal of Agriculture and Food Chemistry, 49: 4429–4435.
Parekh, J. and Chanda, S. 2007. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turkish Journal of Biology, 31: 53-58.
Cai, Y. Z., Sun, M. and Corke, H. 1998. Colorant properties and stability of Amaranthus betacyanin pigments. Journal of Agricultural and Food Chemistry, 46: 4491–4495.
Lee, S. C., Kim, J. H., Jeong, S. M., Kim, D. R., Ha J. U., Nam, K. C. and Ahn, D. U. 2003. Effect of far infrared radiation on the antioxidant activity of rice hulls. Journal of Agricultural and Food Chemistry, 51(15): 4400-4403.
Attia, G. Y., Moussa, M. E. M. and Sheashea, E. R. 2013. Characterization of red pigments extracted from red beet (Beta vulgaris L.) and its potential uses as antioxidant and natural food colorants. Egyptian Journal of Agricultural Research, 91(3): 2013.
Sturzoiu, A., Stroescu, M., Stoica, A. and Dobre, A. 2011. Betanine extraction from beta vulgaris-experimental research and statistical modeling. UPB Scientific Bulletin Series B: and Materials Science,73(1): 145-156.
Fathordoobady, F., Mirhosseini, H., Salamat, J. and Abd Manap, M. Y. 2016. Effect of solvent type and ratio on betacyanins and antioxidant activity of extracts from Hylocereus polyrhizus flesh and peel by supercritical fluid extraction and solvent extraction. Food chemistry, 202: 70-80.
Yong, M., Ning, H. and Liu, H. 2006. Exploitation and composition of pumpkin powder. Food Science and Technology, 6: 299– 301.