In vitro antioxidant activity of Cayratia mollissima - an endangered species of Western Ghats of India
Keywords:
Cayratia mollissima, Ethanol extract,, aqueous extract, , DPPH assay,, FRAP assayAbstract
India's tropic climate is versatile to grow colorful Vegetables and Fruits. The colourful fruits and vegetables are loaded with Vitamins, minerals, and Phytochemicals. Amongst the Various Fruits and Vegetables, one of the rarest and endangered fruit is Cayratia mollissima, which is used by a community of people called Havyaka Brahmins on a special occasion called Aati Amavasya. Using DPPH and Ferric reducing power as an antioxidant model, the antioxidant potential of aqueous and ethanol extracts of Cayratia mollissima fruit were found out to explore its health benefits. The findings from this In vitro study revealed that Cayratia mollissima fruit extracts could serve as Oxygen radical inhibitors. Evaluation of TAC-Total Anti-oxidant Capacity of the extracts were done using FRAP and DPPH assay method. To measure a drug’s potency, the Half-maximal inhibitory concentration (IC50) is broadly used. The TAC of aqueous extract and ethanol extract through the FRAP assay method is IC50=354.25 µg and IC50=189.56 µg respectively. The TAC of aqueous extract and ethanol extract through the DPPH assay method is IC50=184.94 µg and IC50=163.88 µg respectively.
References
Ali, Y. M., Iqbal, S. Z., Shah, A., and Ahmed. M. J. 2003. Effect of different combinations of nitrogen, phosphorous, and farm yard manure on yield and quality of strawberry. Sarhad Journal of Agriculture, 19: 185-188.
Alam, A. H. M. K., Rahman, M. A. A., Baki, M. A., Rashid, M. H., Bhuyan, M. S. A., and Sadik, M. G. 2002. Antidiarrhoeal principle of Achyranthes ferruginea Roxb. and their cytotoxic evaluation. Bangladesh Pharmaceutical Journal, 12: 1–4.
Benzie, I. F. F., and Strain, J. J.1999. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol. 299:15–23. doi: 10.1016/S0076-6879(99)99005-5.
Blois, M. S. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181: 1199–2000. "Cayratia mollissima (Wallich.) Gagnep". Glob in Med. Retrieved 11 August 2014.
Desmarchelier, C., Bermudez, M. J. N., Coussio, J., Ciccia, G., and Boveris, A. 1997. Antioxidant and prooxidant activities in aqueous extracts of Argentine plants. International Journal of Pharmacognosy, 35(2):116–120.
Huda, A. W. N., Munira, M. A. S., Fitrya, S. D. and Salmah, M. 2009. Antioxidant activity of Aquilaria malaccensis (Thymelaeaceae) leaves. Pharmacognosy Research. 1(5): 270–2733.
https://en.wikipedia.org/wiki/Cayratia_mollissima
Leong, C. N. A., Tako, M., Hanashiro, I. and Tamaki, H.2008. Antioxidant flavonoid glycosides from the leaves of Ficus pumila L. Food Chemistry. 109(2): 415–420.
Siju, E. N., Rajalakshmi, G. R., Kavitha, V. P. and Anju, J. 2010. In Vitro antioxidant activity of Mussaenda frondosa. International Journal of PharmTech Research. 2(2): 1236–1240.
Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M. and Telser, J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology, 39(1):44–84.
