Effect of lysozyme based Opuntia ficus-indica mucilage edible coating on shelf life of papaya (Carica papaya L.) fruits
Keywords:
Edible coating, lysozyme,, Opuntia ficus-indica, S. aureusAbstract
Papaya is a highly perishable fruit with a very short postharvest life due to its susceptibility to mechanical injury, physiological disorders, and infection caused by several pathogens that can rapidly reduce fruit quality. The current investigation aims to assess the impact of Opuntia ficus indica mucilage combined with lysozyme applied as edible coating on papaya fruit quality, sensory indices, and microbiological characteristics during storage at RT. Opuntia ficus-indica fruit polysaccharide was utilised as a component of an edible coating. The five edible antimicrobial coating formulations were created using lysozyme in varied concentrations: 0 (control), 0.2 (T1), 0.4 (T2), 0.6 (T3), 0.8 (T4), and 1 % (T5). The antimicrobial edible coated samples were subjected to sensory, chemical, and microbial evaluation and T5 sample showed higher overall acceptability. The uncoated papaya showed a significantly greater linear rise in weight loss after storage at RT than the coated papaya. The T5 sample was effective in maintaining fruit weight, TSS, pH, and acidity. The lysozyme-mucilage based coating had positive effect on reducing the S. aureus on papaya surface. Therefore, it is concluded that developed antimicrobial edible coating has a potential to reduce microbial load while keeping sensorial qualities and hence could be commercialized.
References
Abebe, Z., Tola, Y. B., and Mohammed, A. 2017. Effects of edible coating materials and stages of maturity at harvest on storage life and quality of tomato (Lycopersicon Esculentum Mill.) fruits. African Journal of Agricultural Research, 12(8): 550- 565.
J. Postharvest Technol., 2023, 11(3): 110-118 116
Yadav et al. (Lysozyme based Opuntia ficus-indica mucilage edible coating for papaya)
Aloui, H., and Khwaldia, K. 2016. Natural antimicrobial edible coatings for microbial safety and food quality enhancement. Comprehensive Reviews in Food Science and Food Safety, 15(6): 1080-1103.
Aneja, K. R. 2007. Experiments in microbiology, plant pathology and biotechnology. New Age International, 195-196.
AOAC. 1990. Official method 981.12. the pH of acidified foods. Official methods of analysis (13th ed.). Washington D.C. AOAC International.
Association of Official Analytical Chemists. Official methods of analysis of A.O.A.C 2016. International (20th ed.). Rockville, USA: AOAC International.
Brishti, F. H., Misir, J., and Sarker, A. 2013. Effect of biopreservatives on storage life of papaya (Carica papaya L.). International Journal of Food Studies, 2(1).
Cunningham, F. E., Proctor, V. A., and Goetsch, S. J. 1991. Egg-white lysozyme as a food preservative: an overview. World's Poultry Science Journal, 47(2): 141-163.
Dhall, R. K. 2013. Advances in edible coatings for fresh fruits and vegetables: a review. Critical Reviews in Food Science and Nutrition, 53(5): 435-450.
Ercan, D., and Demirci, A. 2016. Recent advances for the production and recovery methods of lysozyme. Critical Reviews in Biotechnology, 36(6): 1078-1088.
Gheribi, R., and Khwaldia, K. 2019. Cactus mucilage for food packaging applications. Coatings, 9(10): 655.
Hodges, R. J., Buzby, J. C., and Bennett, B. 2011. Postharvest losses and waste in developed and less developed countries: opportunities to improve resource use. The Journal of Agricultural Science, 149(S1): 37-45.
Lara, G., Yakoubi, S., Villacorta, C. M., Uemura, K., Kobayashi, I., Takahashi, C., and Neves, M. A. 2020. Spray technology applications of xanthan gum-based edible coatings for fresh-cut lotus root (Nelumbo nucifera). Food Research International, 137: 109723.
Liguori, G., Gaglio, R., Settanni, L., Inglese, P., D’Anna, F., and Miceli, A. 2021. Effect of Opuntia ficus-indica Mucilage Edible Coating in Combination with Ascorbic Acid, on Strawberry Fruit Quality during Cold Storage. Journal of Food Quality, 1-8.
Parni, B., and Verma, Y. 2014. Biochemical properties in peel, pulp and seeds of Carica papaya. Plant Archieve, 14(1): 565- 568.
Prasad, K., and Paul, J. R. 2021. Postharvest Losses of Papaya and Practice for Management. Food Science Report, 2(1), 7.
Praseptiangga, D., Utami, R., Khasanah, L. U., and Evirananda, I. P. 2017, February. Effect of cassava starch-based edible coating incorporated with lemongrass essential oil on the quality of papaya MJ9. In IOP Conference Series: Materials Science and Engineering, 176(1): 012054.
Ranganna S. 1986. Handbook of Analysis and Quality Control for Fruit and Vegetable Products. Tata McGraw-Hill Publishing Company, New Delhi, India. pp. 124-125.
J. Postharvest Technol., 2023, 11(3): 110-118 117
Yadav et al. (Lysozyme based Opuntia ficus-indica mucilage edible coating for papaya)
Riaz, S., Sultan, M. T., Sibt-e-Abass, M., Imran, M., Ahmad, R. S., Hussain, M. B., and Egorova, G. N. 2021. Extraction of polysaccharides from opuntia cactus for its potential application in edible coating to improve the shelf life of citrus (Kinnow mandarin) fruit. Journal of Microbiology, Biotechnology and Food Sciences, 745-750.
Sah, S., Johar, V., and Karthi, J. S. 2022. Status and Marketing of Fruits and Vegetables in India: A Review. Asian Journal of Agricultural Extension, Economics and Sociology, 40(7): 1-11.
Sharmin, M. R., Islam, M. N., and Alim, M. A. 2015. Shelf-life enhancement of papaya with aloe vera gel coating at ambient temperature. Journal of the Bangladesh Agricultural University, 13(1): 131-136.
Wang, S., Shao, B., Chang, J., and Rao, P. 2011. Isolation and identification of a plant lysozyme from Momordica charantia L. European Food Research and Technology, 232: 613-619.
Xu, F., Liu, S., Liu, Y., Xu, J., Liu, T., and Dong, S. 2019. Effectiveness of lysozyme coatings and 1-MCP treatments on storage and preservation of kiwifruit. Food Chemistry, 288: 201-207.