Analysis of raw chicken meat for microbiological quality

Authors

  • Kashyap Kumar Baruah 1 School of Allied Medical Sciences, University of Science and Technology, Meghalaya-793101, Meghalaya, India Author
  • Saiyyad Alamdar Husain Department of Applied Biology, University of Science and Technology, Meghalaya-793101, Meghalaya, India Author
  • Md. Meraj Ali Khan Department of Applied Biology, University of Science and Technology, Meghalaya-793101, Meghalaya, India Author

Keywords:

Chicken,, Aeromonas,, Staphylococcus, Salmonella,, food safety

Abstract

A study was conducted with a forty numbers of isolates from twenty fresh chicken meat samples of which 16 (40.00 percent) and 24 (60.00  percent) isolates were used in study. A total of 13 (32.50 percent) strains of coagulase negative Staphylococci, 5 (12.50 per cent) strains of  Aeromonas spp. and 22 (55.00 per cent) strains of Salmonella spp. were isolated. The microbiological quality of chicken meat as observed by  TVC was in the range of 4.70 to 8.60 log10 cfu/g with average TVC of 6.36 ± 1.19 to 6.92 ± 0.35 log10 cfu/g. The analysis of microbes in chickens  muscle, liver, heart, and gizzard samples revealed the TVC (log10 cfu±SE) in the range of 6.02 to 7.80 (average 6.91± 2.13), 5.40 to 7.50  (average 6.45±1.27), 4.70 to 7.80 (average 6.36±1.19) and 4.95 to 8.60 (average 6.92 ±0.35), respectively. The investigation revealed that  prevalence of Aeromonas spp. and coagulase negative Staphylococcus in muscle, liver, heart, gizzard were found to be 20 percent, 20 per  cent 18 per cent and 60 per cent and that of coagulase negative Staphylococcus was 23.06 percent, 23.06 per cent 53.88 per cent and 0 per  cent. The investigation also revealed the prevalence of Salmonella spp. present in muscle (31.88%), liver (31.86%), heart (0.0%) and gizzard (36.24%). The results recorded that 88.88% isolates of Salmonela spp. showed resistance towards Amoxycillin (30mcg) and Ampicillin (10mcg) and 71.42% isolates of Aeromonas spp. showed resistance towards Amoxycillin (30mcg) and Ampicilin (10mcg). Streptomycin (10mcg) showed 100% resistance to Staphylococcus isolates. 

References

A.O.A.C. 2012.. Official Methods of Analytical Chemist. (15th ed.). Meat and Meat Products. Association of Official Analytical Chemist, Virginia. Inc., 931- 948 pp.

American Public Health Association. 1966. Washington D.C. U.S.A.

Anon. 1994. Indian Poultry Industry Year Book. 1994. 10th Edn. Privaashini Vihar, Delhi.

Amara, A., Badou, M., Faid, M., and Bouzoubaa, K. 1994. Microbial contamination of poultry slaughtered in traditional shops in Morocco. MAN Microbiologie, aliments, nutrition, 12(3), 323-327.

Borah, P., Patgiri, G. P., and Boro, B. R. (1992). Bacteriological quality of market pork in Guwahati city. Indian Veterinary Journal, 69(9), 773-775.

C.K., Jayanaram, C.K. and Ananthanarayan, R. 1986. Textbook of Microbology. 4th Edn. Orient Longman, Madras- 600 002.

J. Postharvest Technol., 2023, 11(2): 113-121 119

Baruah et al. (Microbiological quality of raw chicken)

CADRAP, 2006 Salmonellosis. Centre for Infectious Disease Research and Policy, American Health Centre, University of Minnesota, At. www.Cidrap.umm.edu/cidrap/content/fs/food/disease/cause/salmoviews.html

Centers for Disease Control and Prevention (CDC). Preliminary FoodNet Data on the incidence of infection with pathogens transmitted commonly through food—10 states, 2009. MMWR Morb. Mortal Wkly. Rep. J. 2010, 59, 418–422.

Chaiba, A., Rhazi, F. F., Chahlaoui, A., Soulaymani, B. R., and Zerhouni, M. 2007. Microbiological quality of poultry meat on the Meknès market (Morocco). Internet Journal of Food Safety, 9, 67-71.

Chiu CH, Wu TL, Su LH, Chu C, Chia JH, Kuo AJ, Chien MS, Lin TY. 2002. The emergence in Taiwan of fluoroquinolone resistance in Salmonella enterica serotype choleraesuis. The New England Journal of Medicine. 346:413–419.

Clinical and Laboratory Standards Institute: 2012. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Ninth Edition; Wayne, PA, USA,

Crump JA, Luby SP, Mintz ED. 2004. The global burden of typhoid fever. Bulletin of the World Health Organization. 82:346–353. Forest, J.C., Aberie, E.D., Hedrick,H.B., Judge, M.D. and Merked, R.A. 1975. Principles of Meat Science. 19: 859 - 869. Gracey JF 1986. Meat Hygiene. 8th Edition, English Language Book Society. Bailliere Tindall, UK, P. 495-497.

Hayes, W and Freeman, J.F. 1945. The incidence type and bacteriology of Salmonella infection in army in India. India Journal. Medical Res. 33: 177- 193.

ISO, Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for the Detection of Salmonella spp., Vol. 2002, 2002. 4th Edition, Geneva, Switzerland.

Jay,J.M., Loessner, M .J. and Golden, D.A. 2005. Modern Food Microbiology, 7th edition, Springer Science and Business media, Inc., New York, USA.

Mohan, K, Maheswarappa, N.B. and Banerjee, R. 2022. Exploring the dynamics of women consumerpreference, attitude and behavior towards meat and meat products. Meat Science. 193 :108926,

Muyanja, C., Nayiga, L., Brenda, N., and Nasinyama, G. 2011. Practices, knowledge and risk factors of street food vendors in Uganda. Food control, 22(10), 1551-1558.

National Action Plan for Egg and Poultry for Doubling Farmers Income by 2022. Department of Agriculture and Farmers Welfare. Government of India.

Paniker, C.K., Jayanaram, C.K. and Ananthanarayan, R. 1986. Textbook of Microbology.4 th Edn. Orient Longman, Madras 600 002.

Praveen Kumar Praveen .2012. Studies on incidence of Aeromonas species in retail fish and chicken in and around Kolkata and its public health importance. M.V.Sc. Thesis. WBUAFS. Kolkata- 700 037.

S Sandel M.K. and Mckillip, J.L. 2004. “Virulence and recovery of Staphylococcus aureus relevant to the food industry using improvements on traditional approaches,” Food Control, vol. 15, no. 1, pp. 5–10.

J. Postharvest Technol., 2023, 11(2): 113-121 120

Baruah et al. (Microbiological quality of raw chicken)

Sachan, N and Agarwal, R. K. 2000. Selective enrichment broth for the isolation of Aeromonas spp. from chicken meat. International Journal of Food. Microbiology. 60 (1)65-74.

Sharma,I., Kumar, A. and Pramanik, A.K. 2009. Isolation and identification of mesophilic Aeromonas bacteria from Meat and Fish Foods of North- East India. Journal of Pure and Applied Microbiology. 3(2):517-525.

Smith, D.M. 2001.Functional properties of muscle proteins in processes poultry products.A.R. Sams (Ed.), Poultry Meat Processing, CRC Press, p. 186

Tafesse, F., Desse, G., Bacha, K., and Alemayehu, H. 2014. Microbiological quality and safety of street vended raw meat in Jijiga town of Somali Regional State, southeast Ethiopia. African Journal of Microbiology Research, 8(48), 3867- 3874.

Yashoda, K. P., Sachindra, N. M., Sakhare, P. Z., and Rao, D. N. 2001. Microbiological quality of broiler chicken carcasses processed hygienically in a small scale poultry processing unit. Journal of food quality, 24(3), 249-259.

Published

2024-05-17

How to Cite

Baruah, K.K., Husain, S.A., & Khan, M.M.A. (2024). Analysis of raw chicken meat for microbiological quality . Journal of Postharvest Technology, 11(2), 113–121. Retrieved from https://acspublisher.com/journals/index.php/jpht/article/view/14980