Efficacy of New Fungicide and Biopolymer Based Against Root Rot of Safflower Caused by Trichoderma Macrophomina Phaseolina

A M Thikare; M H Shete; R T Gaikwad; N B Pawar

doi:10.48165/jpds.2023.1801.01

Authors

A M Thikare Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri- 413722 (Maharashtra), India.
M H Shete Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri- 413722 (Maharashtra), India
R T Gaikwad Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri- 413722 (Maharashtra), India.
N B Pawar Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri- 413722 (Maharashtra), India.

DOI:

https://doi.org/10.48165/jpds.2023.1801.01

Keywords:

Fungicides, Macrophomina phaseolina, Root rot, Safflower

Abstract

Safflower (Carthamus tinctorius L.) has long been grown in India as a rainfed crop, and the country leads the world in both acreage and production. Diseases are major biotic restrictions in safflower production. Among the diseases, root rot caused by Macrophomina phaseolina is one of the most important safflower diseases, producing significant output losses. In the current work, four novel fungicides and two bioagents were tested in vitro against M. phaseolina. Thiophanate methyl 45% + Pyraclostrobin 5% FS (84.17%) inhibited the most mycelial development among the fungicides, followed by Tebuconazole 5.4% w/w FS (76.67%). However, in terms of bioagent, biopolymer-based Trichoderma harzianum (47.22%) inhibited mycelial growth the most, followed by Trichoderma harzianum Th4d WP (44.44%). In vivo evaluation of new fungicide molecules and bioagents revealed that, Thiophanate methyl 45% + Pyraclostrobin 5% FS was recorded 66.67 per cent wilt disease control followed by Tebuconazole 5.4% w/w FS 55.55% whereas biopolymer based Trichodermaharzianum showed 53.33 per cent wilt control followed by Trichoderma harzianum Th4d WPwith leastpercent disease control (11.12%).

References

Adsul, A.K., 2016: Studies on root rot of safflower incited by Macrophomina phaseolina (Tassi)Goid. Indian Phytopath., 12: 283-294.

Chavan, R.A., 1998: Efficacy of Trichoderma in controlling charcoal rot of safflower. M.Sc. (Agri.). Thesis (Unpub.), Vasantrao Naik Marathwada Agril. University, Parbhani, Pp. 50.

Dennis, C., and J. Webster, 1971: Antagonistic properties of species groups of Trichoderma II production of volatile antibiotics. Trans. Br. Mycol. Soc., 57:41-48.

Deshmukh, R.W., 1981: Charcoal rot of safflower (Carthamus tinctorious L.) caused by M. phaseolina (Tarri.) Goid. M.Sc. (Agri.), Thesis(Unpub.), Vasantrao Naik Marathwada Agril. University, Parbhani, Pp. 39.

Dhingra, O. D., and J. B. Sinclair, 1978: Biology and pathology of M. phaseolina. Universal federal de viosa, vicosa Brazil. Pp: 141-166.

FAO, 2021: Food and Agricultural Organization Report.https://www.fao.org.

Gade, R. M., M. Koche, D. Wasule and Z. Haque, 2021: Soybean seed and seedling blights, rots, bacterial spot and mosaic. In:Diseases of Nationally Important Field Crops (Ed.: M. Rahman Khan, Z. Haque and F. Ahamad), Todays and Tomorrow's Printers and Publishers, New Delhi, Pp: 421-434.

Hooda, I., and R.K. Grover, 1982: Studies on different isolates, age and quantity of inoculums of Rhizoctonia bataticola in relation to disease development in mungbean. Indian Phytopath., 35(4): 619-623.

Ingole, B. M., and S. B. Zade, 2020: Bioassay of different agrochemicals and fertilizers against root rot (Macrophomina phaseolina) of soybean in in vitro. Int. J. Curr. Microbiol. App. Sci.,9(04): 582-589.

Nene, Y. L. and P. N. Thapliyal 1993: Fungicides in Plant Disease Control. Oxford and IBH Publishing House, New Delhi, Pp: 163.

Murumkar, D. R., D.V.Indi, R. D. Prasad, and S. K. Shinde, 2018: Evaluation of biocontrol potential o f t h e r m o to l e r a n Trichoderma a n d Pseudomonas against seed and soil borne

diseases of safflower. J. Oilseeds Res., 35(4): 279-

Prasad, R.D., K. S. V. Chandrika and V. Godbole, 2000: A n ovel c hi tosa n bi op olymer - ba sed Trichoderma delivery system: Storage stability, persistence and bio efficacy against seed and soil borne diseases of oilseed crops. Microbiol. Res., 237.

Vincent, J.M., 1947: Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 159: 850.