Assessing The In Vitro Efficacy Of Agrochemicals And Antagonist Against Major Seed Borne Fungi Of Pearl Millet

Authors

  • Prajakta P. Jadhav Department of Plant Pathology, Dr.PDKV, Akola-444104 (MS)
  • K. A. Deshpande Department of Plant Pathology, Dr.PDKV, Akola-444104 (MS)
  • M. S. Joshi Department of Plant Pathology, Dr.PDKV, Akola-444104 (MS)
  • G. K. Giri Department of Plant Pathology, Dr.PDKV, Akola-444104 (MS)

DOI:

https://doi.org/10.48165/jpds.2022.1702.11

Keywords:

Bioagents, Fungicides, Mycoflora, Pearl millet, Seeds

Abstract

The significant millet crop grown in India is pearl millet, which has a slightly superior amino acid profile and is high in protein. Using blotter paper, four seed-borne fungus were isolated: Alternaria alternata, Curvularia penniseti, Bipolaris setariae, and Fusarium moniliforme. The fungicides and antagonists were then assessed using the poisoned food and dual culture techniques, respectively. Tebuconazole (0.1%), Copper oxychloride (0.25%) were found most effective fungicides over all other treatments for arresting the mycelial growth (100%) of all associated seed borne fungi followed by combi product Carbendazim + Mancozeb (0.25%). The most efficient fungal bioagent against seed-borne fungus among the antagonists was Trichoderma asperellum, followed by Trichoderma harzianum. In comparison to fungal antagonists, bacterial antagonists Bacillus subtilis and Pseudomonas fluorescence were shown to be less potent. 

References

Abdel-Fattah, G.M, M. S. Yasser, A.E. Ismail, and Y. M. Rashad, 2007:Trichoderma harzianum: a biocontrol agent against Bipolaris oryzae. Mycopathologia, 164: 81-89

Bhoyar, P.R., V. D. Chandankar ,V.L. Bagde, D. B. Borkar and J. S. Sonone, 2014: Studies on seed borne mycoflora and effect of bioagents and fungicides on wheat seed health. The Biscon,9 :1285-1289.

Bhushan, G., S.Chhangani,J. Singh and A.P. Singh, 2013: Antagonistic effects of Bacillus subtilis and Pseudomonas fluorescens against seed-borne mycoflora of Pennisetumamericanum. J Environ Sci. Technol., 1(3): 71-75.

Dakshinamoorthy, T. and K. Sivaprakasam ,1992: Assessment of seed microflora of pearl millet and their control. Madras Agricultural J., 79(6):347- 351.

Dennnis, K. L. and J. Webster, 1971: Antagonistic properties of species group of Trichoderma and hyaphal interaction. Trans. British Mycol. Soc., 57:363-396.

Gutha, V.R., K.B.Palanna,B.M. Arunkumar , H.D.Kumar and T.E.Nagaraja, 2021: Assessing the in vitro efficacy of new molecules of fungicides against Bipolarissetariae infecting brown top millet. J. Pharmocogn. Phytochem., 10(2): 1123-1130.

Halgekar, N.Y., G. K. Giri , and C. Ashwini , 2015: Efficacy of fungicides against seed borne fungi in rice. Environment and Ecology, 33(3A): 1276- 1279

Harlapur, S.I., C. Kotramma ,S.Kulkarni and M.C Wali 2016: Etiology, prevalence and management of curvularia leaf spot of maize in Karnataka. Indian Phytopathol 69 (4s): 54-57

Ingle, R.W., and J.G.Raut, 1993: Fungi associated with glume and seed in pearl millet at different seed development stages. Seed Res., 21: 131-132.

Jain, S.C., V.N. Pathak and K.L. Jain, 1996: Effect of fungal toxic metabolites on seed germination and seedling growth of pearl millet. J. Mycol .Plant Pathol., 26 (1): 87-88.

Mandal, P., Z.Tiru , M. Sarkar , A.P.Chakroborty , and A. Pal 2021:Invitro antagonistic study of maize root colonizing fungal isolates against Fusarium monilliforme causing ear rot disease of maize. J. Trop. Med., 11: 2.

Nasnwa, R., S.L. Godara ,G.L.Kakraliya, S. Choudhary,D. Kumar and R. Choudhary, 2017: Evaluation of genotypes of pearl millet against leaf spot disease. Int. J. Chem. Stud., 5(3): 596-599.

Nene, Y. L. and Thapliyal P. N. 1993. Fungicides in Plant Disease Control. Second Edition Oxford & IBH Publishing, New Delhi.pp.531-532.

Patil, P.J., 2015: Studied on grain moulds of sorghum (Sorghum bicolour) .MPKV, Rahuri 8: (3): 13-17.

Vincent, J. M. 1947. Distortation of fungal hyphae in presence of certain inhibitors. Nature, 15:850

Zade, S. B.,Y. V. Ingle,and R. W .Ingle, 2018: Evaluation of fungicides, botanicals and bio-agents against Alternariaalternataincitant of leaf spot of soybean. Journal of Pharmacognosy and Phytochemistry, 7(5), 1687-1690.

Published

2023-03-02

How to Cite

Assessing The In Vitro Efficacy Of Agrochemicals And Antagonist Against Major Seed Borne Fungi Of Pearl Millet. (2023). Journal of Plant Disease Sciences, 17(2), 140–143. https://doi.org/10.48165/jpds.2022.1702.11