DETECTION AND DIAGNOSIS OF SEEDBORNE MYCOFLORA ASSOCIATED WITH PIGEON PEA USING DIFFERENT DETECTION METHODS

H D Ingle; S T  Ingle; N V  Gurav; N V  Chaure

doi:10.48165/jpds.2024.1901.04

Authors

H D Ingle Department of Plant Pathology, Post Graduate Institute, Dr. PDKV, Akola-444 104 (MS), India
S T Ingle Department of Plant Pathology, Post Graduate Institute, Dr. PDKV, Akola-444 104 (MS), India
N V Gurav Department of Plant Pathology, Post Graduate Institute, Dr. PDKV, Akola-444 104 (MS), India
N V Chaure Department of Plant Pathology, Post Graduate Institute, Dr. PDKV, Akola-444 104 (MS), India

DOI:

https://doi.org/10.48165/jpds.2024.1901.04

Abstract

The present study deals with the detection, identification, estimation of mycoflora associated with pigeon pea seeds Fusarium, Phytophthora, Alternaria, Rhizoctonia and Cercospora are the most common fungal pathogen associated with stored seed and aremainly responsible for seed deterioration, reduction in germination potential and seedling. Hence, the present study investigated seed samples of pigeon pea variety, PKV-TARA collected from Pulse Research Unit, Dr. PDKV, Akola (Maharashtra) were tested for the detection and identification of seed borne mycoflora by various methods viz., standard blotter paper method, agar plate method, rolled paper towel method.

References

Chaudhari, A., H. Sharma, M. Jehani, and J.K. Sharma. 2017. Seed mycoflora associated with pigeonpea (Cajanus cajan), their significance and management. Journal of Pure and Applied Microbiology, 11(1): 567-575.

Gupta, A., and R. Kumar. 2020. Management of seed-borne diseases: An integrated approach. Seed-borne Diseases of Agricultural Crops: Detection, Diagnosis, and Management, pp. 717-745.

Kandhare, A.S. 2014. Different seed categories of pigeonpea and its seed mycoflora. International Research Journal of Biological Sciences, 3(7): 74-75.

Kesharwani, A., N. Lakpale, N. Khare, and P. K. Tiwari. 2018. Seed health evaluation of pea varieties by incubation methods. International Journal of Current Microbiology and Applied Sciences, 7(8): 601-611.

Khani, S.Q., M.I. Khaskheli, M.M. Jiskani, I.A. Nizamani, A.J. Khaskheli, X. Chang, and A. Anum. 2019. Association of seed mycoflora with peas (Pisum sativa L.) seeds. International Journal of Environment, Agriculture, and Biotechnology, 4(3): 692-698.

Khilari, P.R., S.J. Magar, and K.B. Vishakha. 2021. Detection of seed mycoflora of pigeonpea by seed health testing methods. The Pharma Innovation Journal, 10(12): 2672-2675.

Mallesh, S.B., V.K. Deshpande, and S.A. Kulkarni. 2008. Effect of seed mycoflora management on seed germination and seedling vigour of pigeonpea during storage. Journal of Ecobiology, 23(1): 77-82.

Nirgude, Y.K., R.R. Rathod, U.R. Phondekar, R. Gaonkar, and N.V. Chaure. 2022. Detection and isolation of seed mycoflora of lablab bean, horse gram, and cowpea, and control of major seed mycoflora. The Pharma Innovation Journal, 11(7): 3768-3774.

Patil, D.P., P.V. Pawar, and S.M. Muley. 2011. Mycoflora associated with pigeonpea and chickpea. International Multidisciplinary Research Journal, 2(6): 10-12.

Pradhan, A., N. Lakpale, and N. Khare. 2016. Comparative efficacy of different incubation methods in detecting seed-borne mycoflora of pigeonpea (Cajanus cajan). Journal of Soils and Crops, 26(1): 45-47.

Sahu, S.K., R.R. Sukhadevrao, S. Sahu, B.K. Sahu, and A.L. Tandon. 2023. Studies on seed-borne mycoflora of soybean seeds by incubation methods. International Journal of Plant & Soil Science, 35(18): 722-739.

Saxena, K.B., R.V. Kumar, and P.V. Rao. 2002. Pigeonpea nutrition and its improvement. Journal of Crop Production, 5(2): 227-260.

Vaidya. 2012. Investigation of pea (Pisum sativum L.) seed mycoflora and its control. Ph.D. Thesis, Tribhuvan University, Kathmandu, Nepal.

Youssef, M.A., A.Z. Aly, M.R.A. Tohamy, and M.I. Ghonim. 2018. Studies on fungi associated with pea seeds and their effect on germination and some seed characters. Zagazig Journal of Agricultural Research, 45(4): 1291-1308.