A COMPREHENSIVE SURVEY OF BBTV INCIDENCE SUPPORTED BY PCR-BASED MOLECULAR VALIDATION

A  D  Gadhave; M  S  Joshi; S  V Sawardekar; P  D Patil; B  D Shinde

doi:10.48165/

Authors

A D Gadhave Department of Plant Pathology, College of Agriculture, Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli- 415712, Ratnagiri (M.S.), India.
M S Joshi Department of Plant Pathology, College of Agriculture, Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli- 415712, Ratnagiri (M.S.), India.
S V Sawardekar Plant Biotechnology Center, College of Agriculture, Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli- 415712, Ratnagiri (M.S.), India.
P D Patil Department of Plant Pathology, Regional Agricultural, Research Station, Karjat, Dist. Raigad, (M.S.), India
B D Shinde Department of Agril. Entomology, College of Agriculture, Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli- 415712, Ratnagiri (M.S.), India.

DOI:

https://doi.org/10.48165/

Keywords:

Banana Bunchy Top Virus, Polymerase chain reaction, Molecular, detection,, Prevalence

Abstract

The Banana Bunchy Top Virus (BBTV) is a deadly disease that threatens global banana production. The purpose of this survey is to determine the prevalence of BBTV in banana plantations and to validate its presence using Polymerase Chain Reaction (PCR) analysis. A comprehensive survey was done throughout various banana-growing regions in Maharashtra state, and the virus was detected using a nucleic acid-based polymerase chain reaction (PCR) approach with four different BBTV specific primer pairs. The findings of this survey provide important information about the prevalence and distribution of BBTV in various banana growing areas. The Palghar district had the greatest disease incidence (2.5%), while Jalgon district had the lowest incidence (0.1%). The BBTV positive samples were detected by all four primerpairs, with band sizes of 350 bp for BBT1 and BBT2, 750 bp for BBT2.2, 1000 bp for DNA-C, and 1050 bp for DNA-U3.The existence of expected size PCR amplicons showed thepresence of BBTV in the obtained samples.

References

Allen, R.N., R.T. Plumb and J. M. Thresh, 1983: Spread of Banana Bunchy Top and other plant virus diseases in time and space. : (Eds.). Blackwell Scientific Publications, UK pp: 51-59.

Amin, I., Qazi, J., S. Mansoor, M. Ilyas, R. B. Briddon, 2008: Molecular characterisation of Banana

Latifah, Hidayat, S.H., K. H. Mutaqin, Widodo and A. Sutanto 2021: Survey of Banana Bunchy Top

Anandhi, J., C. Vijila, G. S. Viswanath, and T. S.

/694/1/012044.

Magee, C. J. P., 1940: Transmission studies on the

Lokeswari, 2007: Screening banana plants for banana bunchy top virus with primers specific to Indian isolates. .,

banana bunchy-top virus. 6:109–10.

J. Aust. Inst. Agric. Sci.,

J. Plant Dis. Prot

:101–107. doi: 10.1007/BF03356715. Anhalt, M. D., R. P. P. Almeida, 2008: Effect of temperature, vector life stage, and plant access period on transmission of Banana bunchy top virus to banana. , 98:743–748.

Phytopathology

Dale, J. L., 1987: Banana bunchy top: an economically important tropical plant virus disease. 33: 301–26.

Adv. Virus Res.,

Das, T., and A. Banerjee ,2018: Distribution, molecular characterization and diversity of Banana Bunchy Top Virus in Tripura, India.

Shweta, 2020: Molecular detection and transmission of Banana Bunchy Top Virus (BBTV) in banana. Thesis submitted to the University of Agricultural Sciences, GKVK, Bangalore(Unpublished). pp. 78.

Thomas, J. E. and R. G. Dietzgen, 1991: Purification, characterization and serological detection of virus-like particles associated with banana bunchy top disease in Australia. 72

J. Gen. Virol.,

(Pt 2):217–224.

Thomas, J. E., and M. L. Iskra-Caruana, 2000: Bunchy Top. : R.D. Jones (ed),

In Diseases of

Virus Dis.

, 29 (2):157–166

Banana, Abaca and Ensete

. CAB International,

Edwards, K., C. Johnstone and C. Thompson, 1991: A simple and rapid method for the preparation

Wallingford, pp. 241–253.

Wardlaw, C.W., 1961: Mosaic, Infectious chlorosis

of plant genomic DNA for PCR analysis.

Nucleic

and other virus diseases. Banana diseases,

Acids Research

, 19(6): 1349

including plantains and abaca. London:

Gibbs, A., 1983: Plant Virus Epidemiology. : The

In

Spread and Control of Insect-Borne Viruses, Plumb, R.T. and J.M. Thresh (Eds.). Blackwell Scientific Publications, UK., pp: 39-50.

Longmans.

Ximba, S.P.F., J. Tshabalala, A. Gubba, 2022: Monitoring the distribution of banana bunchy top virus in South Africa: a country-wide survey.

Arch. Virol.

: 1433–1441.

bunchy top virus (BBTV) from Pakistan.Virus Virus on non-cultivated Bananas in West Java. Genes , 36:191-198. http://dx.doi.org/ Earth Environ. Sci., 694: DOI 10.1088/1755- 10.1007/ s11262-007-0168-y.