Biochemical Indicators for Resistance Against Rhizoctonia Root Rot in Soybean

Y K Belkar; R M Gade; Y V Ingle

doi:10.48165/

Authors

Y K Belkar Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S) 444 104
R M Gade Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S) 444 104
Y V Ingle Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S) 444 104

DOI:

https://doi.org/10.48165/

Keywords:

Rhizoctonia root rot, Soybean, Polyphenol oxidase, Peroxidase, ammonia-lyase

Abstract

Biochemical characterization of soybean cultivars was carried out against Rhizoctonia root rot disease. Study showed significant correlation of disease resistance with biochemical parameters. In absolutely resistant cultivar AMS-1002 total soluble sugar was 5.47 (mg/g fresh wt.) in healthy root sample, which increased up to 5.52 (mg/g fresh wt.) after pathogen inoculation whereas in highly susceptible cultivar TAMS-38 it was 3.64 (mg/g fresh wt.) in healthy root sample but due to infection of pathogen which decreased up to 3.63 (mg/g fresh wt.). Highest concentrations of total phenols were observed in genotypes AMS-1002, AMS-103, AMS-3923 and MAUS-71. Also, AMS 1002 and AMS-103 showed peroxidase content of 14.64 (units/min/g fresh wt.) and 11.50 (units/ min/g fresh wt.) in healthy root, which increased up to 17.40 (units/min/g fresh wt.) and 13.96 (units/ min/g fresh wt.) in infected root. PPO activity ranged from 1.72 units/min/g fresh wt. for genotype MAUS-71 to 4.22 units/min/g fresh wt. for AMS-1002. PAL increased significantly in infected tissues of AMS-1002, AMS-103, AMS-3923 and MAUS-71 genotypes compared to the healthy tissues. Higher phenols, total and reducing sugars content in cultivars coupled with other biochemical components might play a vital role in providing resistance towards root rot in soybean.

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