Antagonistic Potential of Trichoderma Viride Mutants Against Soil Borne Pathogens

A G Gathe; S T Ingle; M P Vyawahare

doi:10.48165/

Authors

A G Gathe Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola- 444104 (M.S.), India
S T Ingle Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola- 444104 (M.S.), India
M P Vyawahare Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola- 444104 (M.S.), India

DOI:

https://doi.org/10.48165/

Keywords:

Mutation, Trichoderma viride, Ethyl Methyl Sulphonate (EMS), Hydroxyl Amine (HA), Sclerotium rolfsii, Rhizoctonia bataticola, Fusarium udum

Abstract

Trichoderma viride mutants which were applied against soil borne plant pathogens were found effective against these pathogens after application of different concentrations chemical mutagens of Ethyl Methyl Sulphonate (EMS) and Hydroxyl Amine (HA) i.e. @ 50, 100,150,200 and 250 µl/ml at time interval of 60 minutes. Ten T. viride mutants were obtained and tested for their antagonistic activities in vitro. Efficient mutants were tested up to six generation to check their stability. Among the mutants, four stable mutants viz.,TvME-4 (T4), TvMH-8 (T8), TvME 3 (T3) and TvMH-9 (T9) were proved as effective antagonists against Sclerotium rolfsii,where as In case of Rhizoctonia bataticola the mutants TvME-4 (T4), TvMH-8 (T8), TvME-3 (T3) and TvMH-10 (T10) showed maximum growth inhibition and Fusarium udum was also inhibited by mutants TvME-3 (T3), TvME-4 (T4), TvMH-9 (T9) and TvMH-8 (T8) .

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