In Vitro Evaluation of Bio Agents and Anti-bacterial Chemicals Against Ralstonia Solanacearum Causing Wilt in Brinjal
DOI:
https://doi.org/10.48165/Keywords:
Ralstonia solanacearu, Pseudomonas fluorescens, brinjal, wiltAbstract
The studies were carried out on bacterial wilt caused by Ralstonia solanacearum on brinjal (Solanum melongena L.) during kharif 2014 at Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani. Bacterial wilt of solanaceous crops is a major limitation on the production of brinjal. An experiment on in vitro studies was carried out to find out the effectiveness of bioagents and anti bacterial chemicals to inhibiting the growth of R. solanacearum. All the different modes of bioagent treatment with different plant parts at 5, 10 and 20 per cent conc. and anti-bacterial chemicals at different levels of application evaluated in vitro (pot culture) on susceptible brinjal cv. Puneri Kateri were found antibacterial against R. solanacearum. However, bactericide combinations viz., copper oxychloride + streptocycline, Copper hydroxide + streptocycline and 2- Bromo, 2- Nitro Propane, 1, 3- Diol + streptocycline recorded significantly highest reduction in mortality 71.16, 64.88 and 55.95 per cent, respectively. Modes of treatment of bioagent P. fluorescens viz., Seed + root + soil and Root + soil recorded significantly highest reduction in mortality 67.16 and 60.71 per cent, respectively.
References
Anonymous, 2016: Indian Horticulture Database: Ed. Chander P. Gandhi Deputy Director (IS), NHB.
Baker K.F. and Cook R. J. 1974: Biological control of soil borne pathogens. (Ed) San Francisco, W.H. Freeman and Co., 433.
Masaoka, Y., M. Kojima, S. Sugihara, T. Yoshihara, M. Koshino and A. Ichihara, 1993: Dissolution of ferric phosphate by alfalfa (Medicago sativa L.) root exudates. Pl. Soil., 156:75-78.
Pankaj, A. K. S. and Pardeep Kumar, 2014: Evaluation of botanicals against Ralstonia solanacearum causing bacterial wilt of solanaceous crops. Pl. Dis. Res., 29(1):53-62.
Sawant, A. P., G. P. and Utpal Dey, 2014: Integrated management of bacterial wilt of brinjal incited by Ralstonia solanacearum. J. Pl. Dis. Sci., 9(2): 1 90-195.
Sawant, A. P., G. P. Jagtap and Utpal Dey, 2015: Efficacy of bactericides against wilt ofbrinjal incited by J. Pl. Dis. Sci.. 10(1):33-38.
Tiwari, R. K. S., S. S. Chandravanshi, B. S. Thakur and S. Singh, 1998: Efficacy of leaf extracts of
medicinal plant species against Xanthomonas campestris pv. campestris causing black
rot of cabbage. Indian Phytopath., 51:315.
Vidyasekaran, P., 1999: Biotechnological approaches to supress seed to plant transmission of seed borne pathogens. In: Nation. Symp. On Seed Science and Technol, 5-7 August, Univ. Mysore, Manasagangotri, Mysore (India).
Xian Yanga, Xue Zhang, Shui-Ping Yanga and Wei-Qi Liuc, 2013: Evaluation of the A n t i b a c t e r i a l Activity of Patchouli Oil. Iranian. J. Pharma. Res., 12(3):307-316.
Yabuchi, E., Y. Kosako, H. Yano Hutta and Y. Nishivehi, 1995: Transfer of two Burkholderia and an Alcaligenes species to Ralstonia Gen. Nov. proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff, 1973) Comb. Nov, R. solanacearum (Smith, 1896) Comb. Nov. and R. cutropha (Davis, 1969) Comb. Nov. Microbiol. Immunol., 39:897-904.