Formulation of Culture Media for Growth of Nitrogen Fixing, Phosphate Soulbilizing and Potash Mobilizing Bacteria in a Consortium

M H Shete; D R Murumkar; A M Tirmaliand; K B Landge

doi:10.48165/

Authors

M H Shete Biological Nitrogen Fixation Scheme, College of Agriculture, Pune
D R Murumkar Biological Nitrogen Fixation Scheme, College of Agriculture, Pune
A M Tirmaliand Biological Nitrogen Fixation Scheme, College of Agriculture, Pune
K B Landge Biological Nitrogen Fixation Scheme, College of Agriculture, Pune

DOI:

https://doi.org/10.48165/

Keywords:

Rhizobium, PSB and KMB, consortia, green gram

Abstract

The present study was concerned with the formulations of suitable culture media for growth of nitrogen fixing, phosphate soulbilizing and potash mobilizing bacteria in a consortium. Among the different culture media, MS III media having Glucose (10 g/L), Mannitol (10 g/L), Ammonium sulphate (0.5 g/L) and Yeast extract (6 g/L) recorded maximum growth and cfu count of Rhizobium, PSB and KMB. These three beneficial microorganisms found compatible with each other when grown on MS III culture media. Seed inoculation of green gram with consortia of MPKV Rhizobium, PSB and KMB +75% RDF significantly increase the number of branches (4.75), Plant height (60.50 cm), number of nodules/plant (41.50) and chlorophyll content (0.8368 mg/g) and found at par with 100% RDF.The maximum number of pods/plant (8.00) yield/plant (16.75g) and dry matter/plant (24.25g) were recorded in treatment of 100% RDF but found at par with seed inoculation with consortia of MPKV Rhizobium, PSB and KMB +75% RDF which recorded number of pods/plant, yield/plant and dry matter/plant 7.25, 15.50 g and 23.50 g, respectively. The significantly highest available ‘N’ 164 kg/ha, ‘P’ 28 kg/ha and ‘K’ 800 kg/ha were recorded in seed inoculation with consortia of MPKVRhizobium, PSB & KMB consortia + 75% RDF followed by 100% RDF.Seed inoculation with consortia of MPKV Rhizobium, PSB and KMB significantly increased the growth and yield of greengram as compared to uninoculated control.

References

Bai, B. P., T. C. Charles, C. Trevor and D. L. Smith, 2002: Co-inoculation dose and root zone temperature for plant growth promoting rhizobacteria on soybean (Glycin max L.) growth in soil-less media. Soil. Biol. Biochem.34: 1953-1957.

Bansal, R. K., 2015: Synergistic effect of Rhizobium, PSB and PGPR on nodulation and grain yield of mungbean. Int. J. Agri. Biol., 15: 55–68.

Fasim F., N. Ahmed, R. Parsons and G. M. Gadd, 2002: Solubilization of Zinc salts by bacterium isolated by the air enviourment of tannery FEMS Micro. Lett 213,1-6.

Ganesan, P. and S. S. Gnanamanickam, 1987: Biological control of Sclerotiumrolfsii sacc.in peanut by inoculation with Pseudomonas fluorescens. Soil Biology and Biochemistry, 19(1): 35-38

Gull, F. Y., I. Hafeez, M. Saleem and K. A. Malik, 2004: Phosphorus uptake and growth promotion of chickpea by co-inoculation of mineral phosphate solubilizing bacteria and a mixed rhizobial culture. Aust. J. Exp. Agric., 44: 623-628.

Gupta, A., A. K. Saxena, M. Gopal and K. V. B. R. Tilak, 1998: Effect of plant growth Promoting rhizobacteria on competitive ability of introduced Brayrhizobium sp. for nodulation. Microbiol. Res., 153: 113-117.

Kucuk. C., M. Kivanc and E. Kinaci, 2006: Characterization of Rhizobium sp. isolated from Bean. Turkey Journal of Biology. 30:127-132.

Martins, A., O. Kimura, S. R. Goi and J. I. Baldani, 2004: Effect of co-inoculation of plant growth promoting rhizobacteria and rhizobia on development of common bean plants (Phaseolus vulgaris, L.). Floresta e Ambiente 11: 33-39.

Paliya, Sonam, A.N. Tikle and Tessy Thomas, 2014: Efficacy of micronutrients in influencing growth behavior of Rhizobium of Pigeonpea (Cajanuscajan L. [Millsp.]) Oriental Journal of Chemistry, 30, No. (2): Pg. 731-736.

Qureshi, M. A, M. A. Shakir, A. Iqbal, N. Akhtar and A. Khan, 2011: Co-inoculation of phosphate solubilizing bacteria and rhizobia for improving growth and yield of mungbean (vignaradiata l.) The Journal of Animal & Plant Sciences, 21(3):