Bio-Priming of Seed for Management of Damping-off of Chilli

R D Pawar; S B Bramhankar; P R Kadu; S A Kakad; Tini Pillai; A  A Labhasetwar; D G Wasnik; S S Bhure

doi:10.48165/

Authors

R D Pawar Section of Plant Pathology, College of Agriculture, Nagpur (M.S.) 440 010
S B Bramhankar Section of Plant Pathology, College of Agriculture, Nagpur (M.S.) 440 010
P R Kadu Section of Plant Pathology, College of Agriculture, Nagpur (M.S.) 440 010
S A Kakad Section of Plant Pathology, College of Agriculture, Nagpur (M.S.) 440 010
Tini Pillai Section of Plant Pathology, College of Agriculture, Nagpur (M.S.) 440 010
A A Labhasetwar Section of Plant Pathology, College of Agriculture, Nagpur (M.S.) 440 010
D G Wasnik Section of Plant Pathology, College of Agriculture, Nagpur (M.S.) 440 010
S S Bhure Section of Plant Pathology, College of Agriculture, Nagpur (M.S.) 440 010

DOI:

https://doi.org/10.48165/

Keywords:

Chilli, damping-off, bio-priming

Abstract

Damping-off caused by Pythium aphanidermatum, in nurseries is a major constraint in chilli production causing plant death, either pre-emergence or post-emergence of seedlings. Present experiment was carried out in in vitro and pot culture to observe the effect of bio-priming with soaking and dry seed treatment with non soaked for bio-agents and metalaxyl against Pythium aphanidermatum. Results revealed that bio-agents, T. harzianum and T. viride recorded maximum mycelial growth inhibition (63.35 and 54.97%, respectively) of the test pathogen and fungicide metalaxyl @ 0.1 and 0.2 per cent was found most effective in inhibition of mycelial growth (100%), of the test pathogen, over untreated control. In seed bio-priming test, significantly least pre-and post-emergence mortality was resulted with metalaxyl (7.40 and 22.22%, respectively), with 76.49 and 76.00 per cent reduction in damping-off incidence, over untreated control, followed by bio-priming with T. harzianum and T. viride.

References

Dennis, L. and J. Webster, 1971: Antagonistic properties of species group of Trichoderma I. Production of non-volatile antibiotics. Trans. British Mycol. Soc., 57: 25-39.

Devi, T. S., M. K. Shivaprakash and C. C. Maina, 2013: Efficacy of seed bio-priming in enhancing seedling vigour of cucumber (Cucumissativas L.) under biotic stress conditions. Mysore J. Agric. Sci., 47(1):107- 111.

El- Mohamedy, R. S. R. and M. M. H. A. EI-Baky, 2008: Effect of seed treatment on control of root rot disease and improvement of growth and yield of pea plants. Middle eastern and Russian J. Pl. Sci. Biotech., 2 (2): 84-90.

El- Mohamedy, R. S. R. and M. A. AbdAlla, 2013: Bio priming seed treatment for biological control of soil borne fungi causing root rot of green bean (Phaseolus vulgaris L.) J. Agril. Tech., 9 (3): 589-599.

Gholve, V. M., V. R. Tatikundalwar, A. P. Suryawanshi and U. Dey, 2014: Effect of fungicides, plant extracts / botanicals and bioagents against damping-off in brinjal. African J. Microbiol. Res., 8 (30): 2835-2848.

Gulhane, V. G., S. J. Gaikwad, P. W. Lanje, S. R. Zade and V. B. Kueuwanshi, 2005 : In vitro study of antagonistic effect of bioagents on damping-off pathogen of tomato. J. Soil Crop.,15 (1): 173-176.

Jain, R. K., P. K. Singh, A. Vaishampayan and S. Parihar, 2014: Management of damping off (Pythium aphanidermatum) in chilli (Capsicum annum cv VNS 4) by Pseudomonas fluorescens. Internat. J. Agri. Env. Biotech., 7 (1): 83-86.

Manoranjitham, S. K., V. Prakasam, K. Rajappan and G. Amutha, 2000 : Control of chilli damping off using bioagents. J. Mycol. Pl. Path., 30 (2): 225-228.

Manoranjitham, S. K., V. Prakasam and K. Rajappan, 2001. Biocontrol of damping off of tomato caused by Pythium aphanidermatum. Indian Phytopath. 54 (1): 59-61.

Mehetre, S. T. and S. P. Kale, 2011: Comparative efficacy of thermophilic bacterium, Bacillus licheniformis (NR1005) and antagonistic fungi, Trichoderma harzianum to control Pythium aphanidermatum induced damping oû in chilli (Capsicum annuum L.). Arch. Phytopath. Plant Prot., 44 (11): 1068-1074.

Muthukumar, A., 2011: Evaluation of introduced Trichoderma species as antagonist of Pythium aphanidermatum causing chilli damping-off. Pl. Dis. Res., 26 (2): 136-138.

Muthukumar, A. and P. Sharma, 2012 : Confrontation assay for Trichoderma as a potential biocontrol agent against Pythiumaphanidermatum andSclerotiasclerotium. Pest Manag. Hort. Ecosys.,18 (1): 74-77.

Nene, Y. L. and P. N. Thapliyal, 1993: Fungicides in plant disease control (3rded.). Oxford and IBH Pub. Ltd. Patel, J. K, K. R. Joshi, H. N. Prajapati and N. P. Jage, 2014 : Bait techniques for isolation of Pythium aphanidermatum causing damping-off of chilli from soil and efficacy of bio-agents in vitro. Trends Biosci., 7 (6): 474-476.

Rajendrakumar, R., B. Thakore and P. K. Rai, 2009 : Bioagents: an approach for the control of damping off of tomato caused by Pythium aphanidermatum. Prog. Agric., 9 (1): 141-143.

Ramanujam, B., R. D. Prasad, S. Sriram and R. Rangeswaran, 2010: Mass production, formulation, quality control and delivery of Trichoderma for plant disease management. J. Pl. Prot. Sci., 2(2):1-8.

Sathya, S., S. Lakshmi and S. Nakkeeran, 2016 : Combined effect of bio-priming and polymer coating against chilli damping off. Internat. J. Agril. Sci. Res., 6 (3): 45-54.

Sawant, I. S. and A. N. Mukhopadhyay, 1990 : Control of damping off of sugarbeet by seed treatment with metalaxyl. Indian Phytopath., 43 (3): 408-413.

Sowmini, R., 1961 : Phycomycetes in agricultural soils with special reference to Pythiaceae. M.Sc. (Agri) thesis, University of Madras: 160.

Vincent, J. M.,1927 : Distortion of fungal hypha in presence of certain inhibitor. Nature., 159:180. Yadav, D. L. and K. R. Joshi, 2012 : Efficacy of agrochemicals against Pythium aphanidermatum cause damping-off in bidi tobacco. J. Pl. Dis. Sci., 7 (1): 77-80.

Zagade, S. N., G. D. Deshpande, D. B. Gawade, A. A. Atnoorkar and S. V. Pawar,2012 : Biocontrol agents and fungicides for management of damping off in chilli. World J. Agril. Sci., 8 (6): 590-597.

Zalte, A., R. M. Gade, A. V. Shitole and Y. K. Belkar, 2013 : Management of damping offbyusingplant growth promoting microorganisms. J. Pl. Dis. Sci., 8(2): 200- 203.