Soil Physico-Chemicaland Biological Parameters Associated With Suppessiveness Againstsclerotinia Sclerotiorum

Tasvina R Borah; S Dutta; A R Barman; K Sen; Shikha Pathak; Krishna Ray

doi:10.48165/

Authors

Tasvina R Borah Department of Plant Pathology, BCKV, Mohanpur, Nadia, West Bengal
S Dutta Department of Plant Pathology, BCKV, Mohanpur, Nadia, West Bengal
A R Barman Department of Plant Pathology, BCKV, Mohanpur, Nadia, West Bengal
K Sen Department of Plant Pathology, BCKV, Mohanpur, Nadia, West Bengal
Shikha Pathak Department of Plant Pathology, BCKV, Mohanpur, Nadia, West Bengal;
Krishna Ray Department of Plant Pathology, BCKV, Mohanpur, Nadia, West Bengal;

DOI:

https://doi.org/10.48165/

Keywords:

Sclerotinia sclerotiorum, soil suppressiveness, soil physico chemical properties, soil biological parameters

Abstract

In recent years, interest in exploitation of intrinsic potential of the soil crop system to manage the pathogens as in suppressive soils has gained grounds over the relatively unreliable control with conventional methods and concerns about pesticide residues. Suppressiveness of 54 soil samples from the states of West Bengal, Assam and Nagaland was maximum during summer season. Of the soil samples only 12.50% samples from Nadia district of West Bengal showed strong suppressiveness against Sclerotinia sclerotiorum with maximum inhibition of 25.39±26.80. Physico-chemical properties showed that the soils from West Bengal were neutral to slightly alkaline with pH of 6.90±0.68 and exchangeable cation (EC) of 0.21±0.09µs/cm. The soils from Assam and Nagaland were acidic with pH of 5.59±0.49 and 5.22±0.33 respectively. Among the soil biological parameters, microbial activity (FDA hydrolysis), MBC, population of Pseudomonads and actinomycetes were found to be the most important predictor of soil suppressiveness against S. sclerotiorum. These four soil biological parameters conjointly could able to explain nearly 74.2 % of variation of soil suppressiveness against S. sclerotiorum.

References

Abawi, G. S. and Grogan, R. G. 1979: Epidemiology of disease caused by Sclerotinia species. Phytopathology 69:899-904.

Barnett, S.J., Roget, D.K. and Ryder, M.H. 2006. Suppression of Rhizoctonia solani AG-8 induced disease on wheat by the interaction between Pantoea, Exiguobacterium and Microbacteria. Australian Journal of Soil Research 44:331-342.

Bolton, M.D., Thomma B.P.H.J. and Nelson B.D. 2006: Sclerotinia sclerotiorum (Lib.) de Bary: biology and molecular traits of a cosmopolitan pathogen. Molecular Plant Pathology 7:1–16.

Bonilla, N.; Cazorla, F.M.; Martínez-Alonso, M.; Hermoso, J.M.; González-Fernández, J.; Gaju, N.; Landa, B.B.; de Vicente, A. 2012.Organic amendments and land management affect bacterial community composition, diversity and biomass in avocado crop soils. Plant Soil 357: 215–226.

Bruton, B.D. and Reuveni, R. 1985. Vertical distribution of microsclerotia of Macrophomina phaseolina under various soil types and host crops. Agriculture, Ecosystems and Environment 12: 165- 169.

Chandrashekara, C., Bhatt, J.C., Kumar, R. and Chandrashekara, K.N. 2012:Supressive soils in plant disease management. In: Singh, V.K., Singh, Y., Singh, A. (Eds.), EcoFriendly Innovative Approaches in Plant Disease Management. International Book Distributers, Dehradun, pp. 241– 256.

Chen, W., Hoitink, A.J., Schmitthenner, A.F., Tuovinen. O.H. 1988. The role of microbial activity in suppression of damping-off caused by Pythium ultimum. Phytopathology 78:314-322.

Dutta, S., Borah, Tasvina R., Barman, A. Roy, Hansda, S. and Ghosh, P. P. 2016: Overview of Sclerotiniasclerotiorum in India with special reference to its emerging severity in Eastern Region. Indian Phytopathlogy 69(4s): 260-265.

Garbeva, P., van Veen, J.A. and van Elsas, J.D. 2008. Rhizosphere Microbial Community and Its Response to Plant Species and Soil History Plant and Soil 302(1):19-32.

Ghini, R. and Morandi, M.A.B. 2006: Biotic and abiotic factors associated with soil suppressiveness to Rhizoctoniasolani. Scientia Agricola 63(2): 153- 160.

Ghini, R. andZaroni, M.M.H.2001:Relação entre coberturasvegetais esupressividade de solos a Rhizoctonia solani. FitopatologiaBrasileira, 26:10-15.

Gu, Y-H. and Mazzola, M. 2001. Impact of carbon starvation on stress resistance, survival in soil habitats and biocontrol ability of Pseudomonas putida strain 2C8. Soil Biology and Biochemestry 33:1155–1162.

Gupta, A.K., Choudhary, R., Bashyal, B.M. and Solanki, I.S. 2016: Incidence and characterization of pigeonpea stem rot disease caused by Sclerotiniasclerotiorum (Lib.) de Bary in North Eastern plain zone of India. Legume Research 39(6): 1021-1027.

Hao, J.J., Subbarao, K.V. and Duniway, J.M. 2003: Germination of Sclerotinia minor and S. sclerotiorumsclerotia under various soil moisture and temperature combinations. Phytopathology 93:443-50.

Huang, H.C. and Kokko, E.G. 1989: Effect of temperature on melanization and myceliogenic germination of sclerotia of Sclerotiniasclerotiorum. Canadian Journal of Botany67:1387-1394.

Huang, H.C. and Kozub, G.C. 1993: Influence of inoculum production temperature on carpogenic germination of sclerotia of Sclerotiniasclerotiorum. Canadian Journal of. Microbiology 39: 548–550.

Kim, J.O., and Mueller, C.W. 1978: Introduction to Factor Analysis: What It Is and How to Do It. Sage Publications, Beverly Hills, CA 75pp.

Manjunatha, N., Devi Prameela, T., Prabhakaran, N., Navali, Gurdevi V. and Patil and Swasthi S. 2014: Morphological and molecular diverisity of Sclerotiniasclerotiorum (Lib.) de Bary isolates of India. The Bioscan9(4): 1763-1767.

Martin, F.N. and Hancock, J. G. 1986. Association of chemical and biological factors in soils

suppressive to Pythium ultimum. Phytopathology 76: 1221–1231.

Mazzola, M. 2007: Manipulation of rhizosphere bacterial communities to induce suppressive soils. Journal of Nematology39(3):213-220.

Mendes, R., Kruijt, M., de Bruijn, I., Dekkers, E., van der Voort, M., Schneider, J.H.M., Piceno, Y.M., DeSantis, T.Z., Andersen, G.L., Bakker, P.A.H.M. and Raaijmakers J.M. 2011. Deciphering the rhizosphere microbiome for disease-suppressive bacteria. Science 332: 1097-1100

Ott, L. 1988: An Introduction to Statistical Methods and Data Analysis, 3rd ed. PWS-KENT Publishing Co., Boston.

Pandey, D., Agrawal, M. and Bohra, J.S., 2015: Assessment of soil quality under different tillage practices during wheat cultivation: soil enzymes and microbial biomass. Chemical Ecology31: 510–523.

Purdy, L.H. 1979:Sclerotinia sclerotiorum : history, diseases and symptomatology, host range, geographic distribution, and impact. Phytopathology69: 875–880.

Saharan, G.S. and Mehta, N. 2008: Economic Importance. In: Sclerotinia diseases of crop plants: biology, ecology and disease management. Springer, Dordrecht (Pub.): 41-45.

Sexton, A.C., Whitten, A.R. and Howlett, B.J. 2006: Population structure of Sclerotiniasclerotiorum in an Australian canola field at flowering and stem infection stages of the disease cycle. Genome49(11):1408–1415.

Sharma, S.K., Sharma, B. and Sharma, P.N. 1999: Evaluation of chickpea germplasm against stem rot caused by Sclerotiniasclerotiorum (Lib) de Bary. Life Science Reporter1: 80-81.

Vance, E.D., Brookes, P.C. and Jenkinson, D.S. 1987. An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry 19:703– 707.

Voroney, R.P. and Paul, E.A. 1984: Determination of kC and kN in situ for calibration of the chloroform fumigation-incubation method. Soil Biology and Biochemistry16:9–14.

Willetts, H.J. and Wong, J.A.L. 1980: The biology of Sclerotiniasclerotiorum, S. trifoliorum, and S. minor with emphasis on specific nomenclature. The Botanical Review 46:101-165.

Wiseman, B.M., Neate, S.M., Keller, K.O. and Smith S. E. 1996. Suppression of Rhizoctonia solani anastomosis group 8 in Australia and its biological nature. Soil Biology and Biochemistry 28(6): 727- 732.

Yanar, Y. and Onaran, A. 2011: Mycelial compatibility groups and pathogenicity of Sclerotiniasclerotiorum(Lib.) De Bary causal agent of white mold disease of greenhouse grown cucumber in Antalya-Turkey. African Journal of Biotechnology10: 3739–3746.