Cultural And Biochemical Varibility Of Xanthomonas Axonopodis Pv. Punicae Causative Agent Of Bacterial Blight Of Pomegranate

Trupti S Chormale; R W Ingle; G K Giri; Ankita B  Anmod; Rekha MRathod Rathod

doi:10.48165/

Authors

Trupti S Chormale Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola-444014 (MS)
R W Ingle Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola-444014 (MS)
G K Giri Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola-444014 (MS)
Ankita B Anmod Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola-444014 (MS
Rekha MRathod Rathod Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola-444014 (MS)

DOI:

https://doi.org/10.48165/

Keywords:

Biochemical, Cultural, Pomegranate, Variations, X. axonopodis pv. punicae

Abstract

Bacterial blight disease of pomegranate caused by Xanthomonas axonopodis pv. punicae is one of the most destructive disease inflicting considerable quantitative and qualitative losses. Cultural and biochemical variability of eight isolates of X. axonopodis pv. punicae (Xap), collected from diverse agro- climatic zone consisting eight district of Maharashtra, India were studied. Out of the nine isolates tested their cultural and biochemical variations, most of them showed variability in colour, size, shape, elevation and appearance on NA media. Xap-2 had found highly pathogenic as shown more lesion size i.e. 0.9 mm after 15 days and 3.4 mm after 21 days of inoculation however, Xap-5 showed lowest lesion size 0.2mm after 15 days and 1.5 mm after 21 days of inoculation. The studies on biochemical characteristics of X. axonopodis pv. punicae showed their positive reactions for gelatine liquification, catalase test, potassium hydroxide (KOH) solubility test, H2S Production, tolerance to 1% NaCl, and starch hydrolysis whereas, negative response for Gram staining, indole production and methyl red test. Isolates Xap-2, Xap-3, Xap-4, Xap-5 grew moderate at pH 6.0 and other isolates Xap-1, Xap-6, Xap-7 and Xap-8 showed less growth at pH 6.0. It is considered that distinct variability existed among the X. axonopodis pv. punicae isolates collected from different locations/Districts of the Maharashtra.

References

Anonymous, 2018: Horticultural Statistics at Glance, National Horticulture Board, Ministry of Agriculture, Government of India.

Benagi, V. I. and M. R. Kumar, 2011: Present status of pomegranate bacterial blight and its management. Acta Hort., 890:475-480.

Chand, R. and R. Kishun, 1991: Studies of bacterial blight Xanthomonas campestris pv. punicae of pomegranate. Indian Phytopath., 44 (3):370-372.

Dhander, D. G., P. Nallathambi, R. D. Rawal and D. M. Sawant, 2004: Bacterial leaf and fruit spot. A new threat to pomegranate orchards in Maharashtra state. A paper presented in 26th Ann. Conf. and Symp. ISMPP, Goa University, Goa, India. pp: 39-40.

Giri, M. S., 2009: Morphological and molecular variability of Xanthomonas axonopodis pv. punicae causing bacterial blight of pomegranate. M.Sc. (Agri.) Thesis (unpub.) Univ. of Agri. Sci., Dharwad, pp:114.

Goszczynska, T., J. J. Serfontein and S. A. Serfontein, 1994: Safrinet manual for phytobacteriology. Introduction to practical Phytobacteriology

Gour, H. N., J. Ashiya, B. L. Mali and Ranjan Nath, 2000: Influence of temperature and pH on the growth and toxin production by Xanthomonas axonopodis pv. vignicola inciting leaf blight of cowpea. J. Mycol. Pl. Path., 30(3): 389-392.

Jyoti, S. V., M. Krishnappa, and K. M. Reddy, 2005: Detection of X. axanopodis pv. punicae on pomegranate (Punica granatum) in Karnataka. J. Mycol. Pl. Pathol., 35(3): 518-519.

Kabade, S. H., R. W. Ingle, P. M. Gore, P. N. Madavi, 2020: Cultural, morphological and biochemical studies of Xanthomonas axonopodis pv. punicae: The causative agent of oily spot in pomegranate. Int. J. Chem. Studies, 8(4): 2518-2523.

Manjula, C. P., 2002: Studies on bacterial blight of pomegranate (Punica granatum) caused by Xanthomonas axonopodis pv. punicae. M.Sc. (Agri.) Thesis, (Unpub.) Univ. of Agri. Sci., Bangalore. pp: 98.

Mondal, K. K. and A. Kumar, 2011: Phenotype based identification of Xap.: Practical Manual on Advances in Phenomics, Genomics and Diagnostics of Xap Causing Bacterial Blight of Pomegranate, pp: 5-11.

Mondal, K. K. and C. Mani, 2012: Investigation of the antibacterial properties of nanocopper against Xanthomonas axonopodis pv. punicae, the incitant of pomegranate bacterial blight. Annals of Microbiology, 62: 889-893.

Patil, A. G., C. V. Ambadkar, K. M. Kanase and V. S. Kashid, 2017: Cultural and morphological characteristics of different Xanthomonas axonopodis pv. punicae isolates on nutrient agar media. Int. J. Curr. Microbiol. App. Sci., 6(11): 1678-1683.

Petersen, Y. E., L. Mansvelt, E. Venter, and W. E. Langenhoven, 2007: Detection of Xanthomonas axonopodis pv. punicae causing bacterial blight on pomegranate in South Africa. J. of Austrian Pl. Path., 39: 544-546.

Ridgway, Robert, 1912: Color standards and color nomenclature Washington, DC, New York, NY Columbia University Libraries, NNC. Columbia University Libraries Electronic Books.