Cultural And Physiological Characterization Of Beauveria Bassiana

Authors

  • Priya B Nimbekar Plant Pathology Section, College of Agriculture, Nagpur
  • S B Bramhankar Plant Pathology Section, College of Agriculture, Nagpur
  • D T Nagrale Plant Pathology Section, College of Agriculture, Nagpur
  • R M Wadaskar Plant Pathology Section, College of Agriculture, Nagpur

DOI:

https://doi.org/10.48165/

Keywords:

Beauveria bassiana, Growth, Humidity, pH, Media, Temperature

Abstract

Effect of different media, temperature, humidity and pH were assessed on the basis of radial growth of Beauveria bassiana (NGP-Bb4). SDAY medium (44.00 mm) supported highest mycelia growth of isolate followed by SDA (43.33 mm). The abundant sporulation and maximum radial growth 42.00 mm was recorded at temperature 25°C. The moderate sporulation and least radial growth 28.00 mm was at temperature 35°C. At 95% relative humidity, highest radial growth of 41.66 mm was recorded till 10th day of incubation with abundant sporulation. Data in respect of pH effect revealed that the highest radial growth of 42.67 mm was achieved after 10th days at pH of 6.0 with abundant sporulation. It can be concluded that, SDA medium and temperature 25°C, pH 6.0 and humidity 95 per cent were considered as optimum for growth and sporulation of B. bassiana. 

References

Alali, S., V. Mereghetti, F. Faoro, S. Bocchi, F. A. Azmeh and M. Montagna, 2019: Thermotolerant isolates of Beauveria bassiana as potential control agent of insect pest in subtrophical climates. J. Pone, 22: 31- 39.

Alves, S. B., S. H. Risco and L. C. Almeida, 1984: Influence of photoperiod and temperature on the development and sporulation of Metarhizium anisopliae (Metsch.) Sorok. Journal of Applied Entomology, 97 (1-5): 127-129.

Bugeme, D. V., N. K. Maniania, M. Knapp and H. I. Bogga, 2008: Effect of temperature on virulence of Beauveria bassiana and Metarhizium anisopliae isolates to Tetranychus evansi. J. Experimental. Appl. Acarology, 46(4): 275-285.

Daniel, R. S. G. and S. B. Alves, 2000: Temperature and relative humidity requirements for conidiogenesis of Beauveria bassiana (Deuteromycetes: Moniliaceae). An. Soc. Entomol. Brazil.,29 (3): 515- 521.

Deb, L., R. Thangaswamy and M. Hajong, 2017: Growth of Beauveria bassiana in different solid media. Trends Biosci., 10 (23): 4815-4817.

Gupta, S. D. and B. Kumar, 2014: Bioefficacy of Beauveria bassiana against third instar larvae of Spodoptera litura. Int. J. Pl. Sci., 9 (1): 97-100.

Gopalkrishnan, C. and Mohan, K. S. 2002: Field efficacy of entomopathogenic fungus Nomuraea rileyi (Farlow) Samson against Spodoptera litura (Fabricius) in beet root. In : Biopesticides and Pest Management Vol. 2 (Eds. Opender Koul, G. S. Dhaliwal, S. S. Marwaha and J. K. Arora) Campus Books International, New Delhi, pp 254 – 259.

Hallsworth, J. E and N. Magan, 1996: Culture age, temperature and pH affect the polyol and trehalose contents of fungal propagules. American Society Microbiol., 62(7): 2435-2442.

Karthikeyan, A., V. Shanthi and A. Nagasthya, 2008: Effect of different media and pH on the growth of Beauveria bassiana and its parasitism on leaf eating caterpillars. Res. J. Agri. Bio. Sci., 4 (2):117-119.

Lingappa, S., H. Saxena and P. S. Devi Vimala, 2005: Role of biocontrol agents in management of Helicoverpa armigera (Huber). In: Recent advances in Helicoverpa armigera management. Ind. Soc. Pulses. Res. Development, IIPR, Kanpur: 159-184.

Miêtkiewski, R., C. Tkaczuk, M. Zurek, L. P. S. Va n de r Geest, 1994: Temperature requirements of four entomopathogenic fungi. Acta Mycol., 29 (1): 109– 120.

Mohammad A. A., I. Ghazal and L. H. Rajab, 2016. Laboratory evaluation of the effect of temperature and several media on the radial growth, conidia production and germination of the fungus B. bassiana. (Bals.). SSRG Int. J. Agri. Env. Sci., 3 (6): 35-41.

Nadeau, M. P., G. B. Dunphy and J. L. Boisvert, 1996: Development of Erynia conica on the cuticle of the adult black flies Simulium rostratum and Simulium decorum. J. Invertebrates Pathol., 68: 50-58.

Padmini, P. P. C. and V. Padmaja, 2010: Impact of different relative humidities on in vitro growth and sporulation of entomopathogenic fungal isolates of Beauveria species. Int. J. Pharmaceutical. Biol. Archives, 1(4): 355-359.

Senthamizhlselvan, P., J. Alice, R. P. Sujeetha and C. Jeyalakshmi, 2010: Growth, sporulation and biomass production of native entomopathogenic fungal isolates on a suitable medium. J. Biopesticides, 3 (2): 466-469.

Shipp, J. L., Y. Zhang, D. W. A. Hunt and G. Ferguson, 2003. Greenhouse microclimate on the efficacy of Beauveria bassiana (Balsamo) for the control of greenhouse arthropod pests. Environ. Entomol., 32 (5): 1154-1163.

Solomon, M. E., 1951: Control of humidity with potassium hydroxide, sulphuric acid or other solutions. Bull. Ento. Res., 42: 543-554.

Ying, S. H and M. G. Feng, 2006. Medium components and culture conditions affect the thermotolerance of aerial conidia of fungal biocontrol agent Beauveria bassiana. Letters Appl. Microbiol., 43:331- 335.

Published

2021-05-02

How to Cite

Cultural And Physiological Characterization Of Beauveria Bassiana . (2021). Journal of Plant Disease Sciences, 16(1), 52–56. https://doi.org/10.48165/