Effect Of Seed Inoculation With Endomycorrhiza And Lipo Chitooligosaccharides On Growth And Yield Of Chickpea (Cicer Arietinum L.)

M D Giri; M N Ingole; N K Patke; S S Hadole

doi:10.48165/

Authors

M D Giri Pulses Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola- 444104 (Maharashtra) India
M N Ingole Pulses Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola- 444104 (Maharashtra) India
N K Patke Pulses Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola- 444104 (Maharashtra) India
S S Hadole Pulses Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola- 444104 (Maharashtra) India

DOI:

https://doi.org/10.48165/

Keywords:

Chickpea, Endomycorrhiza, Lipo chitooligosaccharides, PSB, Rhizobium, Seed inoculation

Abstract

A field experiment was carried out at the research farm of Pulse Research Unit, Washim Road Farm, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during the winter season of 2019-20 on a vertisol soil, to study the response of chickpea (Cicer arietinum L.) to endomycorrhiza and lipo-chitooligosaccharides (LCO). Results indicated that seed inoculation with endomycorrhiza (4g) + lipo-chitooligosaccharides (0.33ml) kg/seed recorded the significantly higher number of root nodules per plant at 30 and 60 DAS, plant height, number of branches per plants and pods per plant in desi chickpea crop. The effect of seed inoculation treatment on microbial count (Spores/g soil @ x105) at 50% flowering, enzyme activity dehydrogenase and phosphatase were found significant. Seed inoculation with endomycorrhiza (4g) + lipo-chittooligodaccaride (0.33ml) recorded the significantly higher values of these parameters than the rest of the treatments. Similarly, the same treatment recorded significantly higher seed yield of desi chickpea.

References

Chen, C., J. McIver, Y. Yang, Y. Bai, B. Schultz and A. McIver, 2007: Foliar application of lipo chitooligosaccharides (Nod factors) to tomato (Lycopersicon esculentum) enhances flowering and fruit production. Can. J. Plant Sci.,87: 365– 372.

Denarie, J. and J. Cullimore, 1993: Lipo-oligosaccharide nodulation factors: a minireview new class of signaling molecules mediating recognition and morphogenesis. Cell,74: 951–954.

Erman, M., S. Demirb,E. Ocakc, S. Tüfenkci, F. Oguz, A. Akkopru, 2011: Effects of Rhizobium, arbuscular mycorrhiza and whey applications on some properties in chickpea (Cicer arietinum L.) under irrigated and rainfed conditions, yield components, nodulation and AMF colonization. Field Crops Research,122:14–24.

Ferguson, Brett James and Mathesius Ulrike, 2003: Signaling interactions during nodule development. J. Plant Growth Regulation,22: 47-72.

Inui, H., Y. Yamaguchiand S. Hirano, 1997: Elicitor actions of N-acetylchitooligosaccharides and laminario ligosaccharides for chitinase and L-phenylalanine ammonia-lyase induction in rice suspension culture. Biosci. Biotechnol. Biochem., 61 : 975– 978.

Mandal, A., A. K. Patra, D. Singh, A. Swarup and R. E. Masto, 2007: Effect of long-term application of manure and fertilizer on biological and biochemical activities in soil during crop development stages. Bioresour Technol.,98:3585– 3592.

OlahBoglarka, Christian Briere, Guillaume Becard, Jean Denarie and Clare Gough, 2005: Nod factors and a diffusible factor from arbuscular mycorrhizal fungi stimulate lateral root formation in Medicago truncatula via the DMI1/DMI2 signalling pathway. The Plant Journal, 44: 195-207.

Panse, V.G. and P. V. Sukhatme, 1995: Statistical Methods for Agricultural Workers, ICAR, New Delhi.

Patke, N.K., R. S. Wankhede and Sonal Nage, 2020: Evaluation of bio-efficacy of endomycorrhiza and lipo- oligosaccharides as seed treatment on chickpea. Int. J. Curr. Microbiol.App. Sci.9 (10): 659-664.

Prithivaraj, B., X. Zhou, A. Souleimanov, W. M. Kahn and D. L. Smith, 2003: A host specific bacteria –to plant signal molecule (Nod factor) enhances germination and early growth of diverse crop plants. Planta., 216: 437-445.

Prudent, M., C. Salon, A. Souleimanov, R. J. N. Emery and D. L. Smith, 2014: Soybean is less impacted by water stress using Bradyrhizobium japonicum and thuricin-17 from Bacillus thuringiensis. Agron. Sustain. Dev.,35: 749–757.

Rocha, I., Y. Ma, P. SouzaAlonso, M. Vosátka, H. Freitas and R. S. Oliveira, 2019: Seed Coating: A Tool for Delivering Beneficial Microbes to Agricultural Crops. Front. Plant Sci., 10:1357.

Schmidt, J., H. Rohrig, M. John, U. Wieneke, G. Stacey and C. Koncz, 1993: Alteration of plant growth and development by Rhizobium nod A and nod B genes involved in the synthesis of oligosaccharide signal molecules. Plant J.,4: 651–658.

Schultze, M., and A. Kondorosi, 1998: Regulation of symbiotic root nodule development. Annu. Rev. Genet.32: 33–57.

Tanaka, K., S. H. Cho, H. Lee, A. Q. Pham, J. M. Batek and S. Cui, 2015: Effect of lipo-chitooligosaccharide on early growth of C4 grass seedlings. J. Exp. Bot.66: 5727–5738.

Wang, N., W. Khan and D. L. Smith , 2012: Changes in soybean global gene expression after application of Lipo-chitooligosaccharidefrom Bradyrhizobium

japonicum under sub-optimal temperature. PLoS ONE7: e31571.