Induced Mutation Of Fludioxonil Resistance In Penicillium Expansum Through Mutagens

R N Baviskar

doi:10.48165/

Authors

R N Baviskar Department of Botany, ICLES’ Motilal Jhunjhunwala College, Vashi, Navi Mumbai (Maharashtra), India

DOI:

https://doi.org/10.48165/

Keywords:

Fludioxonil, Penicillium expansum, Mutagens

Abstract

Isolation of Penicillium expansum from infected apple fruits and their sensitivity was tested and found to be resistance and sensitive. These sensitive isolates of Penicillium expansum was used for further studies. Sensitive isolate (Pe-10) conidia of Penicillium expansum were treated with fludioxonil and various Physical and chemical treatment viz. spontaneous mutation (Sp), Ultraviolet rays (UV) and chemical mutagens such as Sodium Azide (SA), Ethyl Methane Sulphonate (EMS) and N-Nitro-N- Methyl Urea (NMU) due to the sensitive isolate of Penicillium expansum (Pe-10) was found that highly resistance, moderately resistance and few were resistance, highly resistance mutant were more pathogenic. It was seen that UV rays treatment gave higher percentage mutants followed by SP, NMU and EMS. Development of fungicide resistance in the resistant and sensitive isolates was studied by spontaneous mutation, UV, SA, EMS and MU gave higher percentage frequency and higher number of mutants than other treatments. Maximum mutants obtained through spontaneous, UV, EMS-treatment were also stated and highly pathogenic.

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Table 4. Sensitivity of Penicillium expansum isolates from saltation plate against fludioxonil

S.N. Treatments ED 50(µg/ml) MIC(µg/ml) RF 1. Pe-sp-1 0982 1965.9 3.022 2. Pe-sp-3 0970 1940.8 2.983 3. Pe-sp-5 0986 1971.6 3.030 4. Pe-UV-1 1941 3884.5 5.971 5. Pe-UV-4 1457 2915.6 4.482 6. Pe-UV-5 1459 2910.8 4.474 7. Pe-SA-1 0974 1946.4 2.992 8. Pe-SA-2 1455 2911.5 4.475 9. Pe-SA-4 0978 1943.5 2.987 10. Pe-SA-6 1459 2919.4 4.487 11. Pe-EMS-3 1460 2910.2 4.473 12. Pe-EMS-4 9730 1964.1 3.019 13. Pe-EMS-6 1452 2913.2 4.478 14. Pe-EMS-7 1455 2919.4 4.487 15. Pe-EMS-10 1940 3880.2 5.964 16. Pe-EMS-11 2426 4920.5 7.466 17. Pe-EMS-12 1942 3886.4 5.974 18. Pe-NMU-3 1459 2919.2 4.487 19. Pe-NMU-6 1457 2921.5 4.491 20. Pe-NMU-7 1944 3889.3 5.978 21. Pe-10 290.6 0650.5 1.000

ED50 - Fungicide concentration causing 50 % reduction in radial growth

MIC - Minimal Inhibitory Concentration RF - Resistance Factor

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