Organic Compounds and Inorganic Metals Removal from Wastewater Using Klebsiella pneumoniae and Acinetobacter lwoffii
DOI:
https://doi.org/10.48165/Keywords:
Abu Rawash Wastewater Plant, Klebsiella pneumonia, Acinetobacter lwoffii, Heavy Metal, Mineral Silica Oil (MSO), Tryptic Soy Agar (TSA)Abstract
Wastewater contains high levels of organic material, numerous pathogenic microorganisms, as well as nutrients and toxic compounds. Wastewater samples were collected from influent of different wastewater treatment plant. Klebsiella pneumoniae and Acinetobacter lwoffii were isolated from the samples and these isolates were screened for the re duction of wastewater components which estimated by COD (Chemical Oxygen Demand), BOD (Biological Oxygen Demand), and TSS (Total Suspended Solids) values. Wastes reduction by the isolated bacteria using different pH values, incubation temperatures, inoculum volume, static and dynamic condition with different incubation periods and bioremedia ion the variable concentrations of metals in singles and mixtures states were studied. Our investigate show the optimum conditions at pH 7, 35˚C, dynamic condition, 0.5% standard inoculum volume for K.pneumoniae and 2% for A.lwoffii, 48 hours’ incubation period, and metals concentra tions 5ppm.
References
• Amenaghawon, N.A.; Asegame, P.A. and Obahiag bon, K.O. (2013): “Potential Application of Urea and NPK 15:15:15 Fertilizers as Biostimulants in the Bioremediation of Domestic Wastewater”. American Journal of Environmental Protection. 1(4): 91.
• Balaji, V.; Datta, S. and Bhattacharjee, C. (2005): Evaluation on biological treatment for industrial wastewater.IE (I) Journal.CH. 85: 39.
• Bécares E. (2006): Limnology of natural systems for
wastewater treatment, Ten years of experiences at the experimental field for low-cost sanitation in Mansilla de lasMulas, León, Spain. Limnética, 25, 143.
• Brix, H. (1994): Constructed wetlands for municipal wastewater treatment in Europe, In: Mitsch, W.J. ed itor. Global Wetlands: Old World and New, Amster dam, the Netherlands, Elsevier, 325.
• Chuang, S.H.; Ouyang, C.F. and Yuang, H.C. (1997): Effect of SRT and DO on nutrient removal in a combined as-biolfilm process. Wat.Sci.Tech. 36(12): 2019.
• Davies, P. S. (2005): The Biological Basis of Waste water Treatment. West of Scotland Science Park, Strathkelvin Instruments: 1-3.
• Easa, A. and Abou-Rayan, A. (2010): Domestic wastewater effect on the pollution of the groundwater in rural areas in Egypt. Fourteenth International Water Technology Conference, Cairo, Egypt.
• El Shahawy, M.R. (2007): Enhanced aerobic deg radation of some toxic hydrocarbon pollutant. Ph.D., in sciences of Microbiology. Faculty of Science, Ain Shams University, Egypt.
• ESCWA (2010): Waste-Water Treatment Technolo gies–A General Review, Economic and Eocial Com mission for Western Asia, United Nations, New York (ESCWA). http://www. escwa. un. org/information/ publications/edit/upload/sdpd-03-6. pdf accessed.
• Eugene, W. R.; Rodger, B. B.; Andrew D. E. and Lenore S. C. (2012): Standard Methods for the ex amination of Water and Wastewater, 22nd Edition, American Public Health Association, American Wa ter Works Association, Water Environment Federa tion, USA.
• Faryal, R. and Hameed, A. (2005): Isolation and characterization of various fungal strains from tex tile effluent for their use in bioremediation. Pakistani Journal of Botany, 37(4): 1003.
• FEW and APHA, (2012): Standard Methods for the Examination of Water and Wastewater, 22nd Ed., Federation Water Environment (FEW) and American Public Health Association(APHA), Washington, DC, USA.
• Fouda, A., Hassan, S., Azab, M. S and Saied, E. (2016): Decolorization of different azo dyes and de toxification of dyeing wastewater by Pseudomonas stutzeri (SB_13) isolated from textile dyes effluent. British Biotechnology Journal. 15(4): 1.
• Hassan, S., Fouda, A., Azab, M. S and Saied, E. (2015): Biological decolorization of different azo dyes using two bacterial strains of Klebsiella spp. and their consortium. Inter. J. Environ. Biolo. 5(4): 104.
• Imran, H. (2007): Performance evaluation of bio simulator for treating domestic wastewater using ac tivated sludge treatment system J. Appl. Sci. Environ. Manage. 11 (2): 129.
• Kessington, O.O; Andrew, N.A. and Elvis O.A. (2014): Effect of initial pH on the bioremediation of crude oil polluted water using a consortium of mi crobes. The Pacific Journal of Science and Technol ogy.15 (1): 452.
• Lin, S. D. (2007): Water and Wastewater Calcula tions Manual. C. C. Lee. United States, McGraw-Hill Companies: 531.
• Mara, D.D., Mills,S.W., Pearson, H.W. and Ala baster, G.P. (1992): Waste stabilization ponds, A vi able alternative for small community treatment sys tems. JIWEM 6, 8.
• Mohamed, A.G.; El Safty, A.M. and Siha, M.S. (2013): Current situation of water pollution and its effect on aquatic life in Egypt. Egyptian Journal of Occupational Medicine. 37 (1) : 95.
• Nadirah, I.; Zaharah, I.; Zaiton, A.M.; Siti Hary ani, C.K. and Fadhil, M. (2008): Application of Bioparticle in Biofilter for Wastewater Treatment. In-
ternational Conference and Expo on Environmental Management and Technologies, (ICEEMAT), 10-12 2008. Proceeding Booklet.
• Oghenerobor, B.A; Gladys, O.O. and Tomilola, D.O.(2014): Heavy metal pollutants in wastewater effluents: Sources,effects and remediation. Advances in Bioscience and Bioengineering.2(4): 37.
• Puigagut, J.; Villaseñor, J.; Salas, J.J.; Bécares, E. and García, J. (2007): Subsurface-flow constructed wetlands in Spain for the sanitation of small commu nities: a comparative study. Ecol Eng. (30):9.
• Rajesh, S.; Narsi, R.; Bishnoi; Anita, K. and Ra jender, K. (2013): Synergism of Pseudomonas aeru ginosa and Fe0 for treatment of heavy metal contami nated effluents using small scale laboratory reactor.
Bioresource Technology.127: 49.
• Ravi, B.k.; Lakshmi, P.M.; Srinivasarao, D. and Sambasiva, R.A.O. (2013): Bioremediation of sew age using specific consortium of Microorganisms In ternational Journal of Research in Applied. 1 (6): 15.
• Sinha, R.K.; Valani, D.; Sinha, S.; Singh, S. and Herat, S. (2009): Bioremediation of contaminated sites: A low-cost nature’s biotechnology for envi ronmental cleanup by versatile microbes, plants and earthworms. In Faerber, T. and Herzog, J. (Eds), Solid Waste Management and Environmental Reme diation.
• Vymazal, J. (2002): The use of sub-surface con structed wetlands for wastewater treatment in the Czech Republic, 10 years’ experience. Ecological Engineering. 18(5), 633.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
