Unveiling the Biochemical, Enzymatic and Hormonal Stress Biomarkers: Effects of Transportation and Lairage on Sheep Welfare and the Meat Quality
DOI:
https://doi.org/10.48165/jms.2023.180101Keywords:
Welfare, Stress Biomarkers, Transportation, Lairage, SheepAbstract
The objective of this study was to assess the impact of transportation and lairage on biochemical, enzymatic, hormonal changes, and meat quality in Nellore Jodipi sheep. Blood samples were collected from two groups of sheep, L0 (slaughtered immediately after transportation without rest) and L12 (12 h of lairage) during exsanguination to analyze biochemical (blood glucose, creatinine, and total protein), enzymatic (creatine kinase [CK], lactate dehydrogenase [LDH], aspartate transaminase [AST], and alanine aminotransferase [ALT]), and hormonal (cortisol, triiodothyronine [T3], and thyroxine [T4]) parameters. Meat samples from the longissimus dorsi muscle were examined for pH, r value, instrumental color, water holding capacity (WHC), shear force, drip loss, cooking loss, and thiobarbituric acid reactive substances (TBARS). Lower (P<0.05) levels of glucose, creatinine, total protein, LDH, CK, ALT, cortisol, and T4 were noticed in L12. Meat quality parameters indicated higher (P<0.05) pH, r value, and a* values during the post-mortem period in the L0 group compared to L12. However, no difference (P>0.05) was observed for cooking loss, drip loss values between the groups. Current findings suggest that slaughter of sheep immediately after transportation without lairage results in increased stress levels in animals adversely affecting both welfare and meat quality. From the present study, it is concluded that a minimum of 12 h lairage period will allow sheep to regain normal homeostasis.
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References
Aggarwal A, Upadhyay R (2013) Heat Stress and Immune Function. In Heat Stress and Animal Productivity Springer (India) pp. 113–136.
Ali BH, Al-Qarawi AA, Mousa HM (2006) Stress associated with road transportation in desert sheep and goats, and the effect of pretreatment with xylazine or sodium betaine. Research in Veterinary Science 80(3): 343–348.
Apple JK, Dikeman ME, Minton JE, McMurphy RM, Fedde MR, Leith DE, Unruh JA (1995) Effects of restraint and isolation stress and epidural blockade on endocrine and blood metabolite status, muscle glycogen metabolism, and incidence of dark-cutting longissimus muscle of sheep. Journal of Animal Science 73(8): 2295–2307.
Argüello A, Castro N, Capote J, Solomon M (2005) Effects of diet and live weight at slaughter on kid meat quality. Meat Science 70(1): 173–179.
Bond JJ, Can LA, Warner RD (2004) The effect of exercise stress, adrenaline injection and electrical stimulation on changes in quality attributes and proteins in Semimembranosus muscle of lamb. Meat Science 68(3): 469–477.
Bórnez R, Linares MB, Vergara H (2009a) Haematological, hormonal and biochemical blood parameters in lamb: Effect of age and blood sampling time. Livestock Science 121(2): 200–206.
Bórnez R, Linares MB, Vergara H (2009b) Haematological, hormonal and biochemical blood parameters in lamb: Effect of age and blood sampling time. Livestock Science 121(2): 200–206.
Broom DM (2003) Transport stress in cattle and sheep with details of physiological and other indicators. Deutsche Tierärztliche, Wochenschrift 110: 83–89.
Cockram MS (2007) Criteria and potential reasons for maximum journey times for farm animals destined for slaughter. Applied Animal Behaviour Science 106(4): 234–243.
Cockram MS, Kent JE, Goddard PJ, Waran NK, Jackson RE, Mcgilp IM, Southall EL, Amory JR, Mcconnell TI, O’riordan T, Wilkins BS (2000) Behavioural and Physiological Responses of Sheep to 16 h Transport and a Novel Environment Post-transport. The Veterinary Journal 159(2): 139–146.
Cole NA (1995) Influence of a three-day feed and water deprivation period on gut fill, tissue weights, and tissue composition in mature wethers. Journal of Animal Science 73(9): 2548–2557.
Cray C (2012) Chapter 5 - Acute Phase Proteins in Animals. In P. M. Conn (Ed.), Animal Models of Molecular Pathology (Vol. 105, pp. 113–150). Academic Press.
De la Fuente J, Sánchez M, Pérez C, Lauzurica S, Vieira C, González de Chávarri E, Díaz MT (2010) Physiological response and carcass and meat quality of suckling lambs in relation to transport time and stocking density during transport by road. Animal 4(2): 250–258.
Del Campo M, Brito G, Soares de Lima J, Hernández P, Montossi F (2010) Finishing diet, temperament and lairage time effects on carcass and meat quality traits in steers. Meat Science 86(4): 908–914.
Ekiz B, Ergul Ekiz E, Kocak O, Yalcintan H, Yilmaz A (2012) Effect of pre-slaughter management regarding transportation and time in lairage on certain stress parameters, carcass and meat quality characteristics in Kivircik lambs. Meat Science 90(4): 967–976.
Elif EE, Hulya Y (2013) Comparison of Certain Haematological and Biochemical Parameters Regarding Pre-slaughter Stress in Saanen, Maltese, Gokceada and Hair Goat Kids. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 39(2): 189–196.
Ferreira GB, Andrade CL, Costa F, Freitas MQ, Silva TJP, Santos IF (2006) Effects of transport time and rest period on the quality of electrically stimulated male cattle carcasses. Meat Science 74(3): 459–466.
Fisher A, Colditz I, Lee C, Ferguson D (2008) The impact of land transport on animal welfare. Animals in Transit: The Journey Ahead. RSPCA Australia Scientific Seminar.
Fisher AD, Niemeyer DO, Lea JM, Lee C, Paull DR, Reed MT, Ferguson DM (2010) The effects of 12, 30, or 48 hours of road transport on the physiological and behavioral responses of sheep. Journal of Animal Science 88(6): 2144–2152.
Grandin T (1997) Assessment of stress during handling and transport. Journal of Animal Science 75(1), 249–257.
Honikel KO, Fischer C (1977) A rapid method for the detection of PSE and DFD porcine muscle. Journal of Food Science 42(6): 1633–1636.
Horton GMJ, Baldwin JA, Emanuele SM, Wohlt JE, McDowell LR (1996) Performance and blood chemistry in lambs following fasting and transport. Animal Science 62(1): 49–56
Hussnain F, Mahmud A, Mehmood S, Jaspal MH (2020) Influence of Long-Distance Transportation Under Various Crating Densities on Broiler Meat Quality During Hot and Humid Weather. The Journal of Poultry Science 57(3): 246–252.
Kadim IT, Mahgoub O, Al-Kindi A, Al-Marzooqi W, Al-Saqri NM (2006) Effects of transportation at high ambient temperatures on physiological responses, carcass and meat quality characteristics of three breeds of Omani goats. Meat Science 73(4): 626–634.
Kadim IT, Mahgoub O, Al-Marzooqi W, Khalaf S, Al-Sinawi SSH, Al-Amri IS (2009) Effects of transportation during the hot season and low voltage electrical stimulation on histochemical and meat quality characteristics of sheep longissimus muscle. Livestock Science 126(1): 154–161.
Kelly G (2000) Peripheral Metabolism of Thyroid Hormones: A Review Alternative Medicine Review 5(4): 306.
Knowles T, Brown S, Warriss P, Phillips A, Dolan S, Hunt P, Ford J, Edward, J, Watkins P (1995) Effects on sheep of transport by road for up to 24 hours. Veterinary Record 136(17): 431–438.
Knowles TG, Warriss PD, Brown SN, Edwards JE (1998) Effects of stocking density on lambs being transported by road. Veterinary Record 142(19): 503–509.
Leme TM, Da C, Titto EAL, Titto CG, Amadeu CCB, Fantinato Neto P, Vilela RA, Pereira AMF (2012) Influence of transportation methods and pre-slaughter rest periods on cortisol level in lambs. Small Ruminant Research 107(1): 8–11.
Li X, Xia A, Chen L, Du M, Chen L, Kang N, Zhang D (2018) Effects of lairage after transport on post mortem muscle glycolysis, protein phosphorylation and lamb meat quality. Journal of Integrative Agriculture 17(10): 2336–2344.
Liste G, Miranda-de la Lama GC, Campo MM, Villarroel M, Muela E, María GA (2011) Effect of lairage on lamb welfare and meat quality. Animal Production Science 51(10): 952.
Liu HW, Zhong RZ, Zhou DW, Sun HX, Zhao CS (2012) Effects of lairage time after road transport on some blood indicators of welfare and meat quality traits in sheep. Journal of Animal Physiology and Animal Nutrition 96(6): 1127–1135.
Perai AH, Kermanshahi H, Moghaddam HN, Zarban A (2015) Effects of chromium and chromium + vitamin C combination on metabolic, oxidative, and fear responses of broilers transported under summer conditions. International Journal of Biometeorology 59(4): 453–462.
Priolo A, Micol D, Agabriel J, Prache S, Dransfield E (2002) Effect of grass or concentrate feeding systems on lamb carcass and meat quality. Meat Science 62(2): 179–185.
Spinosa H, de S, Górniak SL, Bernardi MM (2006) Farmacologia aplicada à medicina veterinária. Guanabara Koogan.
Tadich N, Gallo C, Brito ML, Broom DM (2009) Effects of weaning and 48 h transport by road and ferry on some blood indicators of welfare in lambs. Livestock Science 121(1): 132–136.
Teke B, Ekiz B, Akdag F, Ugurlu M, Ciftci G, Senturk B (2014) Effects of Stocking Density of Lambs on Biochemical Stress Parameters and Meat Quality Related to Commercial Transportation. Annals of Animal Science 14(3): 611–621.
Vergara H, Linares MB, Berruga MI, Gallego L (2005) Meat quality in suckling lambs: effect of pre-slaughter handling. Meat Science 69(3): 473–478.
Wardlaw FB, McCaskill LH, Action JC (1973) Effect of Postmortem muscle changes on poultry meat Loaf properties. Journal of Food Science 38(3): 421–423.
Zhang L, Yue HY, Zhang HJ, Xu L, Wu SG, Yan HJ, Gong YS, Qi GH (2009) Transport stress in broilers: I. Blood metabolism, glycolytic potential, and meat quality. Poultry Science 88(10): 2033–2041.
Zhong RZ, Liu HW, Zhou DW, Sun HX, Zhao CS (2011) The effects of road transportation on physiological responses and meat quality in sheep differing in age. Journal of Animal Science, 89(11): 3742–3751.
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Copyright (c) 2023 Prasad M Govindaiah, Naveena B Maheswarappa, Rituparna Banerjee, Bidyut Prava Mishra, Balaji B Manohar
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