Response of pH on indigenous biocontrol activity of fungaland bacterial biological agents against Rhizoctonia solani
DOI:
https://doi.org/10.48165/jefa.2023.18.02.27Keywords:
pH, Rhizoctonia solani, Trichoderma harzianum, Fluorescent, Pseudomonas, Aspergillus niger, Penicillium oxalicumAbstract
Root rot incited by Rhizoctonia solani is one the major diseases of pea. As there are no long term management strategies for this soil borne pathogens therefore, the study was carried out to determine the effect of pH on the antagonistic activity of fungal and bacterial isolates (Th3 and Th5 (Trichoderma harzianum), An1 (Aspergillus niger), Po3 (Penicillium oxalicum) and PS1, PS2 and PS5 (Flourescent Pseudomonas) against Rhizoctonia solani at four different pH levels (5.0, 6.0, 7.0 and 8.0). All the seven indigenous fungal and bacterial isolates evaluated for their antagonistic potential against root-rot pathogen, significantly retarded the radial growth of Rhizoctonia solani in comparison to control at four different pH levels (5.0, 6.0, 7.0 and 8.0).The observations of the study revealed that all bio agents significantly inhibited the growth of the Rhizoctonia solani . Similarly, Trichoderma species had significant effects on the number and size of sclerotia. The pH range of 6.0-8.0 were found optimum for Trichoderma spp. while Flourescent Pseudomonas shows best antagonistic activity at pH 7.0. As far as the effect of pH levels is concerned, all the promising fungal and bacterial isolates caused maximum growth inhibition of test pathogens at pH 6.0 to 7.0.
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Aflaq Hamid, Nazir Ahmad Bhat, T. A. Sofi, K. A. Bhat and Malik Asif ( 2012) Management of root rot of pea (Pisum sativum L.) through bioagents African Journal of Microbiology Research Vol. 6(44), pp. 7156-7161, 20 November, 2012
Chattopadhyay C, Meena PD, Sudher K (2002). Management of sclerotinia rot of Indian mustard using ecofriendly strategies. J. Mycol. Plant Pathol. 32:194-200.
Collins, C.H. and Lyne, M.P. (1987) Microbiological methods, butterworth and Co. (Publishers Ltd., 261-265.
Cook, R.J. (1993) Making greater use of introduced microorganism for biological control of pathogens, Ann. Rev. Phytopathology 31: 53-80.
Dennis, C. and Webster, L. (1971a). Antagonistic properties of species-groups of Trichoderma. I. Production of non-volatile antibiotics. Trance.Br. Mycol. Soc. 57: 25-39.
Dennis, C. and Webster, L. (1971b). Antagonistic properties of species-groups of Trichoderma. II. Production of non-volatile antibiotics. Trance.Br. Mycol. Soc. 57: 41-48
Elad, Y., Brak, R., and Chet, I. (1983a) Possible role of lectins in mycoparasitium, J. Bacteriology., 154: 1431-1435.
Elad, Y., Chet, I., Boyle, P., and Hennis, Y. (1983b) Parasitism of Trichoderma spp. on Rhizoctonia solani and sclerotium rolfii Scanning electron microscopy and fluorescence microscopy. Phytopathology 73: 85-88.
Harman, G. E., Chet, I. and Baker, R. (1980). Trichoderma hamatum effects on seed and seedling disease induced in radish and pea by Pythium spp. or Rhizoctonia solani. Phytopathology, 70: 1167-1172.
Harman, G.E., Chet, I., and Baker, R. (1981) Factors afecting Trichoderma harmatum applied to seeds as a biocontrol agents, Phytopathology 71: 569-572.
Harman, G.E., Taylor, A.G. and Stusz, J.E. (1989). Combining effective strains of Trichoderma harzianum and solid matrix priming to improve biological seed treatments. Plant Disease, 73: 631- 637.
Khara, H.S. and Hadwan, H.A. (1989) In vitro studies on antagonism of Trichoderma spp. Plant disease reporter 5(2).
Kloepper, J.W., J. Leong, M. Teintze and M.M. Schroth, (1980a) Pseudomonas siderophores: a mechanism explaining disease-suppressive soil. Curr. Microbiol. 4: 317-320.
Kloepper, J.W., J. Leong, M. Teintze and M.N., Schroth (1980b) Enhanced plant growth by siderophores produced by plant growth promoting rhizobacteria nature 286: 885-886.
Kraft, J.M. (1994) Fusarium wilt of peas (a review) Agronomic 14(9): 561-567, USA.
Kredics, L., Antal, Z. and Manczinger, L. (2000) Influence of water potential on growth, enzyme secretion and in vitro enzyme activities of Trichoderma harzianum at different temperatures. Current Microbiology, 40(5): 310-314.
Kumar D and Dubey SC (2001). Management of collar rot of pea by the integration of biological and chemical methods. Indian Phytopathology, 54:62-66
Markell, S., Pasche, J., and Porter, L., 2016. Pea Disease Diagnostic Series. NDSU,
Mondal, G. (1998) In vitro Evaluation of Aspergillus niger AN 27 against soilborne fungal plant pathogens and field Testing against Macrophomma Phaseolina on Potato. Ph.D. Thesis I.A. R.I., New Delhi p. 117 + XI, VIII.
Mondal, G. Dureja, P. and Sen, B. (2000) Fungal metabolities from Aspergillus niger An 27 related to plant growth promotion. Indian Journal of Experimental Biology 38: 84-87.
Oyarzun, P.J. (1993) Bioassay to assess root-rot in pea and effect of root-rot on yield. Netherland Journal of Plant Pathology pp. 61-75.
Sen, Bineeta (2001) Biological control a success story. Indian Phytopath. 53: 243-249.
Sharma, Ajay, Mathur, P.N. and Mathur Kusum (2005). Production of Chitinase and B-1, 3-glucause lytic enzyme by Trichoderma aureoviride. Indian Phytopathology, 58(3): 326-328.
Singh, R.A., Guraha, S.B. (1996) Disease of mungbean and urdbean and their management. Disease scenario in crop plants, 2: 69-93.
Vincent, J. M. (1947). J. Soc. Chem. Ind. Lond. 25: 149-155
Voisard, C., C. Keel, Haas, D and Defago, G. (1989) Cyanide production by Pseudomonas florescens helps suppress black root rot of tobacco under gnotabiotic conditions. EMBO J., 8: 351-358.
Wang K, Zhang Z, Chen JN, Fan Z, Niu BS Wang KC (1995). A study on the occurrence of root rot of pea (Pisum sativum) and the technique for its integrated control. Niger. J. Agric. For. Sci. Technol. 5:1-5.
Weller, D.M. (1988) Biological Control of soil borne plant pathogens in the rhizosphere. Annu. Rev. Phytopathol, 26: 379-407.
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