A Comparative Analysis of Choorna and Bhavitha Choorna of Flower Buds of Japa (Hibiscus rosa-sinensis Linn.) through High-performance Thin-layer Chromatography

Authors

  • S Smrithi PG Scholar, Department of Dravyaguna Vijnana, Government Ayurveda College, Tripunithura, Kerala, India. Author
  • V V Shincymol Associate Professor, Department of Dravyaguna Vijnana, Government Ayurveda College, Tripunithura, Kerala, India. Author
  • P Y Ansary Professor and HOD, Department of Dravyaguna Vijnana, Government Ayurveda College, Tripunithura, Kerala, India. Author
  • Sara Moncy Oommen Professor and HOD, Department of Dravyaguna Vijnana, Government Ayurveda College, Kannur, Kerala, India. Author

Keywords:

Bhavitha choorna, Choorna, High performance thin layer chromatography, Japakusuma mukula

Abstract

Introduction:High-performance thin-layer chromatography (HPTLC) is nowadays adopted by the major  pharmacopoeias of the world for analysis of herbal drugs and preparations. The current use is generally limited to  the visual observation of the fingerprints for identification and detection of adulterations and falsifications. It makes  sense to use HPTLC to expand chromatographic fingerprints to identify the main active ingredients in medicinal  plants. Compared to thin layer chromatography, the separation and resolution are significantly superior, and the  outcomes are far more consistent and repeatable. Materials and Methods: Japakusuma mukula (flower buds of Japa) which is botanically identified as Hibiscus rosa sinensis Linn. contains various phytoconstituents such as flavonoids, terpenoids, steroids, polysaccharides, alkaloids,  amino acids, lipids, sesquiterpene, quinones, and naphthalene groups. HPTLC fingerprinting profile of choorna (powder) and bhavitha choorna (triturated powder) of flower buds of Hibiscus rosa-sinensis Linn. is demonstrated in  methanolic extract in this study and bands are analyzed at 254 nm and 366 nm using CAMAG Linomat V Automatic  Sample Spotter. The peaks and area are compared. Results and Conclusion: Similarity in the peak areas even in different Max Rf values, it could be inferred that  the phytoconstituent obtained in both conditions will be having similarity. Since the bhavitha choorna possess, the  greater number of large value peak areas in both the wavelength of visualization, it is clear that bhavitha choorna is  more potent in comparison with choorna. 

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References

Attimarad M, Ahmed KK, Aldhubaib BE, Harsha S. High performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery. Pharm Methods 2011;2:71-5.

Rastogi RP. Compendium of Indian Medicinal Plants. Lucknow: Central Drug Research Institute; 2008. p. 381-2.

Mukhopadhyay G, Jain D, Sodani A, Pramanick A, Das AK, Sahoo M. Standardization of quercetin in Hibiscus rosa-sinensis flower by high-performance thin-layer chromatography. Int J Green Pharm 2018;12:S575-8.

Al-Snafi AE. Chemical constituents, pharmacological effects and therapeutic importance of Hibiscus rosa-sinensis-a review. IOSR J Pharm 2018;8:101-19.

Missoum A. An update review on Hibiscus rosa sinensis phytochemistry and medicinal uses. J Ayurvedic Herb Med 2018;4:135-46.

Mohanraj K, Karthikeyan BS, Vivek-Ananth RP, Chand RP, Aparna SR, Mangalapandi P, et al. IMPPAT: A curated database of Indian medicinal plants, phytochemistry and therapeutics. Sci Rep 2018;8:4329.

Nambootiri DS. Chikitsamanjari. In: Asrigdhara Chikitsa. 7th ed. Kodungallor: Vidyarambham Publications; 2005. p.103.

Beegum S. A comparative analysis on kevala amalaki choorna and durva swarasa bhavitha amalaki choorna. Int J Ayurveda 2017; 2:5-9.

Das G. Bhaisajya Ratnavali. Paribhasha Prakarana. Sloka117. Ch. 4. Varanasi: Choukhambha Prakasan; 2008. p. 61.

Poornima M. Phytochemical Screening, Elemental Analysis and Antimicrobial Activity on Flower Part of Three Hibiscus Species. (Masters Thesis). Melmaruvathur: Adhiparasakthi College of Pharmacy; 2012.

Sumathy R, Melanathuru V, Munuswamy S, Sundaram S, Selvaraj ST. A comparative study of in vitro antimicrobial activity and TLC studies of petals of selected Indian medicinal plants. Asian J Pharm Clin Res 2016;9:259-63.

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Published

2023-11-30

How to Cite

Smrithi, S., Shincymol, V.V., Ansary, P.Y., & Oommen, S.M. (2023). A Comparative Analysis of Choorna and Bhavitha Choorna of Flower Buds of Japa (Hibiscus rosa-sinensis Linn.) through High-performance Thin-layer Chromatography . International Research Journal of Ayurveda and Yoga, 6(11), 13–19. Retrieved from https://acspublisher.com/journals/index.php/irjay/article/view/12770