Impact of Different Therapies on Haematological Parameters and Therapeutic Outcome in Subclinical Endometritic Buffaloes
DOI:
https://doi.org/10.48165/ijvsbt.19.5.12Keywords:
Buffalo, Dextrose, Endometritis, Levamisole, PGF2αAbstract
The objective of this study was to compare the effect of administration of intrauterine dextrose, s/c levamisol and intrauterine PGF2α on haematological indices and therapeutic response in endometritic buffaloes. Twenty eight repeat breeder buffaloes with subclinical endometritis were randomly assigned into four groups. Seven animals were kept as Control (untreated C), and the rests were assigned to three treatment group, i.e., T1 (Dextrose I/Ut, 50 mL 4 times on the day of estrus), T2 (Levamisol s/c, @ 2.5 mg/kg b.wt. on day 0, 7 and 14 of the cycle), and T3 (PGF2α I/Ut, @ 0.25 mg in 0.5 mL saline twice, on the day of first and next estrus). Following treatment the values of Hb, TEC and PCV elevated significantly and TLC values dropped to normal or near normal in all three treatment groups as compared to control group. Furthermore, the post-treatment neutrophils and lymphocytes counts were elevated significantly in all four groups. However, there was no any definite pattern of eosinophils and basophils. The therapeutic response (clinical recovery) was 14.29, 71.43, 85.71 and 42.86 % in T0, T1, T2 and T3 group, respectively. The corresponding conception rates were 0.00, 60.00, 66.67 and 66.67 %. The results conclude that, Dextrose intrauterine, Levamisol s/c, and PGF2α intrauterine can be used effectively to manage subclinical endometrtitis in buffaloes.
Downloads
References
Ahmad, I., Gohar, A., Ahmad, N., & Ahmad, M. (2003). Haematological profile in cyclic, non cyclic and endometritic crossbred cattle. International Journal of Agricultural Biology, 5(3), 332-334.
Ahmadi, M.R., Makki, M., Mirzaei, A., & Gheisari, H.R. (2019). Effects of hypertonic dextrose and paraffin solution as non-antibiotic treatments of clinical endometritis on reproductive performance of high producing dairy cows. Reproduction in Domestic Animals, 54(5), 762-771.
Brick, T.A., Schuenemann, G.M., Bas, S., Daniels, J.B., Pinto, C.R., Rings, D.M., & Rajala-Schultz, P.J. (2012). Effect of intrauterine dextrose or antibiotic therapy on reproductive performance of lactating dairy cows diagnosed with clinical endometritis. Journal of Dairy Science, 95(4), 1894-1905.
Butani, M.G., Dhami, A.J., Kumar, R., Hirani, N.D., Ramani, V.P., & Patel, K.P. (2009). Influence of hormonal and antibiotics therapy on fertility and trace minerals profile in repeat breeding buffaloes. Indian Journal of Field Veterinarians, 4(3), 12-16.
Dash, S., Basu, S., Mishra, P.C., & Ray, K. (2019). Effect of immunomodulators on certain haematological parameters in ameliorating bovine Endometritis. International Journal of Chemical Studies, 7(2), 1736-1739.
Dutt, R., Singh, G., Singh, M., Sharma, M., Dalal, J., & Chandolia, R.K. (2017). Diagnosis of subclinical endometritis in Murrah buffaloes through Cytobrush technique. International Journal of Current Microbiology and Applied Sciences, 6(11), 494-499.
Heidarpour, M., Mohri, M., Fallah-Rad, A.H., Shahreza, D.F., & Mohammadi, M. (2014). Hematological changes before and after treatment in dairy cows with clinical and subclinical endometritis. Comparative Clinical Pathology, 23(1), 97-101.
Kasimanickam, R., Duffield, T.F., Foster, R.A., Gartley, C.J., Leslie, K.E., Walton, J.S., & Johnson, W.H. (2004). Endometrial cytology and ultrasonography for the detection of subclinical endometritis in postpartum dairy cows. Theriogenology, 62, 9-23.
Kekan, P.M., Shirbhate, R.N., & Nimbulkar, M.V. (2005). Haematological studies during estrous cycle in regular and repeat breeding cows. Indian Veterinary Journal, 82(7), 805-806.
Kumar, R., Dhami, A.J., Butani, M.G., Kavani, F.S., Sarvaiya, N.P., & Killledar, A. (2010). Management of repeat breeding under field condition using hormonal and non-hormonal drugs in cows. Indian Journal of Animal Reproduction, 31 (1), 30-32.
Le Blanc, S.J., Duffield, T.F., Leslie, K.E., Bateman, K.G., Keefe, G.P., Walton, J.S. & Johnson W.H. (2002). Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows. Journal of Dairy Science, 85, 2223-2236.
Lee, S.C., Jeong, K.J., Choi, I.S., Kang, H.G., Jung, Y.H., Park, S.B., & Kim, I.H. (2018). Cytological endometritis in dairy cows: diagnostic threshold, risk factors, and impact on reproductive performance. Journal of Veterinary Science, 19(2), 301-308.
Lopez-Gatius, F., Yaniz, J. L., Santolaria, P., Murugavel, K., Guijarro, R., Calvo, E., & Lopez-Bejar, M. (2004). Reproductive performance of lactating dairy cows treated with cloprostenol at the time of insemination. Theriogenology, 62(3-4), 677–689. https://doi.org/10.1016/j.theriogenology.2003.11.014
Makki, M., Ahmadi, M.R., Gheisari, H.R., & Nazifi, S. (2017). Cure rate of postpartum endometritis after different treatments in high produce dairy cows. Comparative Clinical Pathology, 26, 921-928.
Maquivar, M. G., Barragan, A. A., Velez, J. S., Bothe, H., & Schuenemann, G. M. (2015). Effect of intrauterine dextrose on reproductive performance of lactating dairy cows diagnosed with purulent vaginal discharge under certified organic management. Journal of dairy science, 98(6), 3876–3886. https://doi.org/10.3168/jds.2014-9081
Patil, R.D., Patel, J.A., Hadiya, K.K., & Dhami, A.J. (2015). Comprehensive haematological alterations following various therapies in Surti buffaloes with postpartum metritis. Indian. Journal of Animal Reproduction, 36(1), 1-4.
Perumal, P., Das, S., Mohanty, D.N., Barik, A.K., & Mishra, P.C. (2013). Study of certain haematological parameters in repeat breeding cows. International Journal of Bio-Resource and Stress Management, 4(2), 242-245.
Reddy, N.C.S., Bramhaiah, K.V., Naidu, K.S., Babu, A.J., & Kumar, R.V.S. (2012). Effect of uterine lavage on bacterial count and conception rate in repeat breeder crossbred cows. Indian Journal of Animal Reproduction, 33(1), 59-62.
Samatha, V., & Ramesh, B.K. (2013). Bacteriological and Cytological studies of endometritis in buffaloes. International Journal of Food, Agriculture and Veterinary Sciences, 3(2), 6-9.
Sheldon, I.M., Lewis, G.S., LeBlanc, S., & Gilbert, R.O. (2006). Defining post partum uterine disease in cattle. Theriogenology, 65, 1516-1530.
Sheldon, I.M., Noakes, D.E., Rycroft, A.N., & Dobson, H. (2004). Effect of intrauterine administration of oestradiol on postpartum uterine bacterial infection in cattle. Animal Reproduction Science, 81, 13-23.
Singh, N., Singh, B., & Kumar, R. (2023). Effect of uterine lavage, levamisole, PGF2α and its combinations on haematological indices and bacterial load in estrual mucus of endometritic buffaloes. The Indian Journal of Veterinary Science and Biotechnology, 19(2), 28-33.
Singh, P.P., Pande, N., Bhavna, & Agrawal, R. (2017). Clinical and biochemical studies on endometrtitic repeat breeding cows following treatment with levamisol. The Haryana Veterinarian, 56(1), 55-57.
Snedecor, G.W., & Cochran, W.G. (1994). Statistical methods. 8th edition, Iowa State University Press, Ames, Iowa, USA.
Thrall, M.A. (2004). Veterinary Haematology and Clinical Chemistry. Published by Lippincott Williams and Wilkins, Philadelphia, p. 71, 84-85.
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Indian Journal of Veterinary Sciences & Biotechnology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
